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Method for improving output of anticancer anthraquinone compound 1403C generated by Fusarium proliferatum No.1403

A technology of endophytic fungi and anthraquinones, applied in the field of metabolic regulation, can solve the problems of difficulties in pharmacological and drug efficacy research, low yield of 1403C, etc., and achieve the effect of increasing yield

Inactive Publication Date: 2010-10-06
EAST CHINA UNIV OF SCI & TECH
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  • Abstract
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Problems solved by technology

[0005] However, in the shake flask fermentation of mangrove endophytic fungus No.1403, the yield of 1403C is extremely low (about 1.3mg / L, see Jiang Guangce, Lin Yongcheng, Zhou Shining, etc. Research on secondary metabolites of mangrove endophyte No.1403 in China[ J]. Journal of Sun Yat-Sen University (Natural Science), 2000, 39(6): 68-72), causing difficulties for further research on pharmacological effects in vivo and in vitro, and it is urgent to develop a method to improve the production of endophytic fungi No.1403 in mangroves. Method for the yield of cancer anthraquinone compound 1403C

Method used

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  • Method for improving output of anticancer anthraquinone compound 1403C generated by Fusarium proliferatum No.1403
  • Method for improving output of anticancer anthraquinone compound 1403C generated by Fusarium proliferatum No.1403
  • Method for improving output of anticancer anthraquinone compound 1403C generated by Fusarium proliferatum No.1403

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Experimental program
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Effect test

Embodiment 1

[0038] The influence of embodiment 1 malonate on thallus growth and 1403C synthesis

[0039] Add different concentrations of malonate to the fermentation broth, the initial addition time is 24 hours, and the final concentrations are 1.5mM, 3mM, 6mM respectively

[0040] The result is as figure 1 As shown, when the final concentration of malonic acid in the fermentation broth reached 1.5, 3, and 6 mM, the growth of the bacteria had no obvious effect on the growth of the control group, but the yield of the product 1403C on the bacteria increased by 3.8%, 68.8% compared with the control, respectively. %, 59.2%, indicating that the addition of malonate during 24 hours of fermentation has a great stimulating effect on the production of product 1403C.

Embodiment 2

[0041] Example 2 The effect of adding sodium acetate and malonic acid on the growth of bacteria and the synthesis of 1403C after mixing according to the molar ratio of 1:1

[0042] Add a mixture of sodium acetate and malonic acid with a molar ratio of 1:1 to the fermentation broth. The initial addition time is 24 hours, and the final concentrations of the mixture in the fermentation broth are 1.5mM, 3mM, and 6mM, respectively.

[0043] The result is as figure 2As shown, when the final concentration of the mixture of sodium acetate and malonic acid in the fermentation broth reached 1.5, 3, and 6mM, the dry weight of the bacteria was 3.8, 3.2, and 3.4g / L, which was slightly lower than the 4.0g / L of the control group. , but the yields of 1403C to bacteria were 0.35, 0.43, and 0.33g / g DCW, respectively, which were 32.7%, 63.5%, and 26.9% higher than the 0.26g / g DCW of the control group, indicating that the molar ratio of 24 hours of fermentation was The 1:1 sodium acetate and ma...

Embodiment 3

[0044] Example 3 Sodium acetate and malonic acid are mixed according to the molar ratio of 1:4 and then added to the effect on the growth of bacteria and the synthesis of 1403C

[0045] Add a mixture of sodium acetate and malonic acid with a molar ratio of 1:4 to the fermentation broth. The initial addition time is 24 hours, and the final concentrations of addition are 1.5mM, 3mM, and 6mM respectively.

[0046] The result is as image 3 As shown, when the final concentration of the mixture of sodium acetate and malonic acid in the fermentation broth reaches 1.5, 3, and 6mM, the dry weight of the bacteria is 3.6, 4.3, and 3.1g / L, which fluctuates around 4.0g / L in the control group. , but the yields of 1403C to bacteria were 0.41, 0.40, and 0.53g / g DCW, respectively, which were 57.3%, 52.3%, and 102.7% higher than the 0.26g / gDCW of the control group, indicating that the molar ratio of 1 : Sodium acetate and malonic acid of 4 have a great stimulating effect on the generation of ...

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Abstract

The invention provides a method for improving output of an anticancer anthraquinone compound 1403C generated by Fusarium proliferatum No.1403. The 1403C fermentation output of the Fusarium proliferatum No.1403 is improved with different degrees by adding malonic acid or mixture of sodium acetate and malonic acid, wherein the maximum improvement is obtained by adding the sodium acetate and the malonic acid in a ratio of 1: 4 and with a final concentration of 6mM, and the maximum yield of 1403C on thalli is 0.53g / g and is improved by 102.7 percent compared with 0.26g / g of the output of a control group. The method has significance for further performing anticancer pharmacological effect experiments of the 1403C.

Description

technical field [0001] The invention belongs to the field of metabolic regulation, and in particular relates to a method for increasing the output of anticancer anthraquinone compound 1403C produced by mangrove endophytic fungus No.1403. Background technique [0002] Mangrove endophytic fungus No.1403 (Fusarium proliferatum) comes from the interior of mangrove leaves in the South China Sea, and was isolated by Professor Vrijmoed of City University of Hong Kong. The research team led by Professor Lin Yongcheng of Sun Yat-Sen University has isolated more than 20 compounds from its fermentation broth, most of which have anti-tumor and antibacterial activities (see Xia Xuekui, Huang Xiaocong, Zhang Jie, Liang Zhibin, Xie Lina, She Zhigang, Lin Yongcheng, Vijmoed LLP. Preliminary optimization of fermentation conditions for the production of griseofulvin by South China Sea mangrove fungus No.1403, Journal of Sun Yat-sen University (Natural Science Edition). 2007, 05: 32-35). [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P15/00C12R1/645
Inventor 周祥山张元兴余超康丽张健邹武
Owner EAST CHINA UNIV OF SCI & TECH
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