Method for improving output of anticancer anthraquinone compound generated by Fusarium proliferatum via utilizing different vaccination ways

A technology of endophytic fungi and mangroves, applied in the field of quinone anticancer compound 1403C production, can solve the problem of low 1403C production and achieve the effect of increasing production

Inactive Publication Date: 2010-05-05
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The main purpose of the present invention is to solve the problem of low yield of 1403...

Method used

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  • Method for improving output of anticancer anthraquinone compound generated by Fusarium proliferatum via utilizing different vaccination ways
  • Method for improving output of anticancer anthraquinone compound generated by Fusarium proliferatum via utilizing different vaccination ways

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 The yield of 1403C obtained by fermentation using inoculation method M1

[0046] Adopt a 500mL Erlenmeyer flask with three baffles (the center of the baffle is 18.5cm away from the mouth of the bottle, 0.5cm away from the bottom of the bottle, and the width of the axial leaf end of the baffle is 0.9cm. The shape of the baffle is trapezoidal, and the baffle is integrated with the main body of the bottle Blown), filled with 100mL seed culture medium, sterilized at 121°C for 20 minutes, picked well-grown seeds from the slant culture medium and put them into the above-mentioned bottle. When the seeds are well grown, grind them aseptically. Inoculated into the fermentation medium with an inoculum of 5%. Such as figure 1 As shown in (M1), the yield of 1403C increased rapidly after 25h, and reached the maximum after 130h of fermentation, which was 1.13g / L, which was 289.7% higher than that of the control group (0.29g / L).

Embodiment 2

[0047] Example 2 The yield of 1403C obtained by fermentation using the inoculation method M2

[0048] Adopt a 500mL Erlenmeyer flask with three baffles (the center of the baffle is 18.5cm away from the mouth of the bottle, 0.5cm away from the bottom of the bottle, and the width of the axial leaf end of the baffle is 0.9cm. The shape of the baffle is trapezoidal, and the conical The baffle in the bottle is integrally blown with the main body of the bottle), filled with 100mL seed medium, sterilized at 121°C for 20min, and picked well-grown seeds from the slant medium and put them into the above-mentioned bottle. After the seeds grow well, they are inserted into the fermentation medium with an inoculation amount of 5%. Such as figure 1 As shown in (M2), the yield of 1403C increased rapidly after 36h, and reached the maximum after 130h of fermentation, which was 0.97g / L, which was 234.5% higher than that of the control group (0.29g / L).

Embodiment 3

[0049] Example 3 The yield of 1403C obtained by fermentation using the inoculation method M3

[0050] Use a 500mL Erlenmeyer flask without baffles, add ten glass beads (diameter 3mm), fill in 100mL seed medium, sterilize at 121°C for 20min, pick well-grown seeds from the slant medium and put them into the above bottle middle. After the seeds grow well, they are inserted into the fermentation medium with an inoculation amount of 5%. Such as figure 1 As shown in (M3), the yield of 1403C increased rapidly after 36h, and reached the maximum after 130h of fermentation, which was 0.8g / L, which was 175.9% higher than that of the control group (0.29g / L).

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Abstract

The invention provides a method for improving output of an anticancer anthraquinone compound 1403C generated by Fusarium proliferatum (No.1403) via utilizing different vaccination ways. The method comprises the following steps: a conical flask with a baffle is adopted to cultivate seeds for grinding inoculation, the seeds cultivated by the conical flask with the baffle are directly inoculated, and seeds cultivated by glass beads are added into the conical flask, therefore, the 1403C yield obtained by fermentation is improved to different degrees compared with the yield obtained by fermentation for cultivating seeds (control group) in a traditional inoculation way, wherein the maximum 1403C yield is 1.13g/L, which is improved by 289.7% compared with 0.29g/L yield of the control group. The invention has vital significance for further performing 1403C anti-cancer pharmacological effect experiments.

Description

technical field [0001] The invention relates to a method for increasing the yield of anthraquinone anticancer compound 1403C produced by endophytic fungus Fusarium proliferatum (No. 1403) by cultivating seeds through six inoculation methods. Background technique [0002] Mangrove endophytic fungi (Endophytic fungi) 1403# is Fusarium proliferatum. It comes from the interior of mangrove leaves in the South China Sea and was isolated by Professor Vrijmoed of City University of Hong Kong. The research team led by Professor Lin Yongcheng of Sun Yat-sen University has isolated more than 20 compounds from its fermentation broth, including griseofulvin and various anthraquinones, naphthoquinones and xanthones Most of these compounds have antitumor and antibacterial activities. 1403C is a novel anthraquinone compound among the isolated compounds (Jiang Guangce, Lin Yongcheng, Zhou Shining, etc. Research on secondary metabolites of endophytic fungus No.1403 in South China Sea mangrov...

Claims

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Application Information

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IPC IPC(8): C12P15/00C12P7/66C12R1/77
Inventor 周祥山张元兴余超康丽孙学谦邹武
Owner EAST CHINA UNIV OF SCI & TECH
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