Bacillus amyloliquefaciens and application thereof

A technology of Bacillus amyloliquefaciens and Bacillus, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of no reports of Bacillus amyloliquefaciens, achieve significant control effects, short production cycle, and broad application prospects

Inactive Publication Date: 2015-11-04
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports of Bacillus amyloliquefaciens that have antagonistic effects o

Method used

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  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Acquisition and identification of Bacillus amyloliquefaciens XK-2, and its antagonism test on watermelon wilt, rice sheath blight and walnut root rot.

[0040] Watermelon Fusarium wilt, rice sheath blight, and walnut root rot described in this embodiment are provided by the Plant Pathology Research Office of the Agricultural College of Changjiang University, and watermelon Fusarium wilt, rice sheath blight, and walnut root rot obtained in other ways are also provided. applicable to the present invention.

[0041]Separation: Collect vigorous watermelon plants from the watermelon planting fields around Jingzhou, Hubei, take their tender roots, wash them with clean water, cut them into small pieces of about 1 cm, put them in a sterilized petri dish, and wash them with 75% alcohol Soak for 50s, then soak with 0.1% mercury liter for 30s, then rinse with sterile water for 4 times. Put it in a sterilized mortar, add a little sterile water, grind, take 1 mL of the ground ba...

Embodiment 2

[0075] Embodiment 2 is basically the same as Embodiment 1, the difference is:

[0076] This embodiment completes the preparation of bacterial strain XK-2 bacterial agent according to the following main steps:

[0077] (1) Inoculate the Bacillus amyloliquefaciens XK-2 bacterial strain obtained in Example 1 on LB solid medium, cultivate at 30° C. for 24 hours, and activate the bacterial strain; LB solid medium preparation: 5 g of yeast extract, 10 g of tryptone, chlorine Sodium chloride 10g, agar powder 20g, NaOH to adjust the pH to 7.2, 121 ℃, 20min autoclaving.

[0078] (2) Insert the strain activated in step (1) into LB liquid medium, shake and culture at 120r / min at 30°C for 24h to make seed liquid; LB liquid medium preparation: 5g of yeast extract, 10g of tryptone , sodium chloride 10g, NaOH to adjust the pH to 7.2, 121 ℃, 20min autoclaving.

[0079] (3) Inoculate the seed fermentation broth obtained in step (2) at 5% by volume in LB liquid fermentation medium, and shake ...

Embodiment 3

[0082] Embodiment 3 is basically the same as Embodiment 1, the difference is:

[0083] This embodiment completes the preparation of bacterial strain XK-2 bacterial agent according to the following main steps:

[0084](1) Inoculate the Bacillus amyloliquefaciens XK-2 bacterial strain obtained in Example 1 on LB solid medium, cultivate at 28° C. for 20 h, and activate the bacterial strain; LB solid medium preparation: 5 g of yeast extract, 10 g of tryptone, chlorine Sodium chloride 10g, agar powder 20g, NaOH to adjust the pH to 7.0, 121 ℃, 20min autoclaving.

[0085] (2) Insert the strain activated in step (1) into LB liquid medium, shake and culture at 120r / min at 28°C for 20h to make seed liquid; LB liquid medium preparation: 5g of yeast extract, 10g of tryptone , sodium chloride 10g, NaOH to adjust the pH to 7.0, 121 ℃, 20min autoclaving.

[0086] (3) Inoculate the seed fermentation broth obtained in step (2) at 5% by volume in LB liquid fermentation medium, and shake and c...

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Abstract

The invention relates to bacillus amyloliquefaciens, the preservation number of which is CCTCC M 2015299, and which is named as bacillus amyloliquefaciens XK-2. The invention further provides a screening method of the bacillus amyloliquefaciens XK-2 and an application of the bacillus amyloliquefaciens XK-2 in inhibiting watermelon fusarium oxysporum, rhizoctonia solani and walnut root rot pathogenic bacteria as well as a bacillus amyloliquefaciens XK-2 bactericide. The bacillus amyloliquefaciens XK-2 is a watermelon endophytic bacterial which is safe to the human beings, animals and crops, environment-friendly, relatively wide in antibacterial spectrum, has a good inhibition effect on common soil-borne pathogen,watermelon fusarium oxysporum, rhizoctonia solani and walnut root rot pathogenic bacteria, has a significant control effect on watermelon fusarium wilt, has an obvious inhibition effect on the growth of watermelon fusarium oxysporum hypha, slows down the growth of hypha, facilitates the distortion syndrome of the hypha and is wide in application prospect in biological control of plant diseases.

Description

technical field [0001] The invention belongs to the technical field of plant disease biological control, in particular to a bacillus amyloliquefaciens and application thereof. Background technique [0002] Watermelon wilt is a soil-borne disease of vascular bundles caused by Fusarium oxysporum f.sp niveum FON. It is one of the most common and most harmful diseases in the watermelon industry. 10%-30%, seriously up to 80%-90%, heavy cropping and even cause extinction. The pathogen invades from the root system of the soil, causing the vascular bundles to become brown and necrotic, leading to the death of melon seedlings. The chlamydospores can survive in the soil for 10 years, seriously affecting the cultivation of watermelons. At present, there is no effective control method for this disease. Although the traditional crop rotation can reduce the occurrence of the disease to a certain extent, with the increase of market demand for watermelon, the planting area of ​​watermelon ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
Inventor 孙正祥周燚邓建新
Owner YANGTZE UNIVERSITY
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