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Disease prevention and growth promotion endophytic bacteria for plants

A plant endophytic bacteria, growth-promoting technology, applied in the direction of bacteria, microorganisms, biocides, etc., can solve the problems of chemical fungicides polluting the environment, difficult to colonize and survive for a long time, and bacteria resistance to drugs, etc., to achieve outstanding control effects, The effect of increasing plant height and promoting plant growth

Inactive Publication Date: 2014-05-07
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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AI Technical Summary

Problems solved by technology

[0002] Plant disease is one of the main factors affecting the high and stable yield and high quality of agriculture. At present, to control the harm of plant diseases, in addition to using the disease resistance of crop varieties, chemical methods are mainly used in the prevention and control. However, chemical fungicides pollute the environment and induce the emergence of pathogens. Drug resistance destroys the ecological balance, and its residues are also worrying. Therefore, the biological control of plant diseases is one of the important ways to make agriculture sustainable, and it has received more and more attention.
Regarding plant disease biocontrol bacteria, most of them were isolated and screened from soil or plant rhizosphere soil microorganisms in the past, but because these soil microorganisms are easily affected by external conditions, they are not easy to settle for a long time in the competition with soil habitant microorganisms. reproductive survival and dominance, thus greatly affecting their actual disease control effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 liquid preparation

[0028] The plant endophytic Paenibacillus polymyxa Jaas ed2 strain is inoculated on the slant of the test tube, the medium is NA, and cultured at a constant temperature of 25° C. for 2-4 days to obtain the test tube species.

[0029] The test tube seed was inoculated into NB medium, cultured overnight at 28°C with constant temperature and shaking, then transferred to NB medium, and the culture solution after 1 day of constant temperature and shaking at 28°C could be the preparation.

[0030] Formula one, common bacteria NA culture medium (weight %)

[0031] Beef extract 0.3%, yeast extract 0.1%, peptone 0.5%, glucose 1.0%, agar 1.5-2.0%, pH6.8-7.0.

[0032] Formula two, common bacteria NB culture medium (weight %)

[0033] Beef extract 0.3%, yeast extract 0.1%, peptone 0.5%, glucose 1.0%, pH6.8-7.0.

Embodiment 2

[0034] The growth promoting effect of embodiment 2 to eggplant seedling

[0035] Sow the germinated eggplant seeds in the greenhouse pots, and when the eggplant seedlings grow to 2 to 3 true leaves, pour the diluted plant endophytic Paenibacillus polymyxa Jaas ed2 bacteria solution on the eggplant seedlings and pot soil After 21 days, the seedling height was measured and the fresh weight was weighed. The test results showed that the bacterial strain had obvious growth-promoting effect on eggplant seedlings, and the plant height increased by 75% compared with the control, and the fresh weight increased by 92% compared with the control.

Embodiment 3

[0036] Embodiment 3 is to the growth-promoting effect of watermelon seedling

[0037] Sow the germinated watermelon seeds in the greenhouse pots, and when the watermelon seedlings grow to 2 to 3 true leaves, pour the diluted plant endophytic Paenibacillus polymyxa Jaas ed2 bacterial solution on the rhizosphere of the watermelon seedlings, and after 20 days The seedling height was measured and the fresh weight was weighed. The test results showed that the strain had obvious growth-promoting effect on watermelon seedlings. The plant height increased by 21.9% and the fresh weight increased by 23.2% compared with the control.

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Abstract

The invention relates to a Paenibacillus polymyxa Jaas ed2 with the functions of disease prevention and growth promotion to crops and application thereof, and belongs to the technical field of intensive agriculture production. The bacterial strain Jaas ed2 adopted by the invention has been preserved at the General Microbiological Culture Collection Center of China Microbiological Culture Collection Administration Committee on Sep. 18th, 2012 with the preservation number of CGMCC No.6580. The bacterial strain has the antagonistic activity to verticillium lecanii of eggplant, oxysporum of watermelon and other various pathogenic fungi, can enter plants of eggplants and watermelons in case of lower concentration of bacterium for colonization and conduction in the crops, can be used for promoting growth and preventing diseases of crops, and particularly has excellent control efficiency to soil-borne vascular bundle diseases, is low in production cost, simple to operate, facilitates ecological environment protection for farmlands, and reduces pesticide residue when used as a preparation, thereby meeting requirements for production of nuisance free agricultural products in China.

Description

Technical field: [0001] The invention relates to a plant endophytic bacterium Paenibacillus polymyxa Jaas ed2 capable of preventing diseases and promoting growth of crops and its application in agriculture, which belongs to the technical field of agricultural intensive production. Background technique: [0002] Plant disease is one of the main factors affecting the high and stable yield and high quality of agriculture. At present, to control the harm of plant diseases, in addition to using the disease resistance of crop varieties, chemical methods are mainly used in the prevention and control. However, chemical fungicides pollute the environment and induce the emergence of pathogens. Drug resistance destroys the ecological balance, and its residual problem is also worrying. Therefore, the biological control of plant diseases is one of the important ways to make agriculture sustainable development, and it has been paid more and more attention. Regarding plant disease biocontr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C12R1/01
Inventor 林玲王卿张昕邓晟
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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