Levo lactone hydrolase producing fungus, and its method for preparing chiral hydroxy acid

Abstract

The invention relates to laevorotation lactone specificity hydrolytic enzyme producing strain and the method used it to make chirality oxyacid. The enzyme producing is Fusarium proliferatum Nirenberg ECU2002 with storage number CGMCC 1494. The chirality oxyacid preparing method includes the following steps: using the fungous mycelium, rough enzyme extract or their immobilization derivative as biocatalyst; processing antipode selectivity hydrolysis resolution for a series of racemic chirality lactone to gain many optically active (+)-oxyacid and (+)-lactone which can be hydrolyzed into (-)-oxyacid; (+)-alpha-hydroxyl-beta, beta-dimethyl-gamma-butyric acid which are D-(+)-pantoic acid; simple acidizing to gain chirality intermediate D-(-)-pantoic acid lactone widely used in preparing feed and daily chemical engineering industry.

Description

technical field

[0001] The invention relates to a high-selectivity L-lactone hydrolase-producing bacterium and a technical method for preparing chiral hydroxyacids or corresponding lactones using the strain. technical background

[0002] Hydroxy acids, such as lactic acid, malic acid, tartaric acid, citric acid and gluconic acid, etc., are a very important class of organic acids, which have unique physiological functions in living organisms and are very useful in the food / feed industry functional additives. This situation is very similar to amino acids, and the two can also be transformed into each other under certain conditions. Some unnatural hydroxy acids, especially optically active chiral hydroxy acids, can also be used as "structural building blocks" for the chemical synthesis of various natural products and drug molecules.

[0003] In addition to pure α-hydroxy acids, β-hydroxy acids, γ-hydroxy acids, and ω-hydroxy acids with hydroxyl at the end can all be dehydrate...

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