Yarrow yeast fa2-3 from the ocean and its low-temperature amylase and enzyme production method

A technology of low-temperature amylase and yarrow yeast, applied in the field of microorganisms, can solve the problems of reducing the efficiency of microorganisms decomposing organic pollutants, etc.

Active Publication Date: 2018-07-06
HUAIHAI INST OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, in some areas with large seasonal changes, the temperature changes greatly, which reduces the efficiency of microorganisms in decomposing organic pollutants such as macromolecular carbohydrates and lipids.

Method used

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  • Yarrow yeast fa2-3 from the ocean and its low-temperature amylase and enzyme production method
  • Yarrow yeast fa2-3 from the ocean and its low-temperature amylase and enzyme production method
  • Yarrow yeast fa2-3 from the ocean and its low-temperature amylase and enzyme production method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Embodiment 1, a kind of Yarrow yeast ( Yarrowia sp . ) FA2-3, CGMCC No. 10377. This bacterial strain has the following characteristics: bacterial strain FA2-3 is oblong, singly disperses, budding reproduction (see figure 1 ). The colonies are white with neat edges. The growth characteristics of strain FA2-3 are as follows: the growth temperature range of the strain is 15-35°C, and the optimum growth temperature is 30°C; the growth pH range is 5.0−10.0, and the optimum growth pH is 7.0; It can grow in the range of 8%, and the optimum growth NaCl concentration is 0.5%.

Embodiment 2

[0081] Embodiment 2, a method for producing low-temperature amylase from Yarrowia FA2-3 from the ocean as described in Example 1, the steps are as follows: inoculate the slant seeds of Yarrowia FA2-3 into YPD medium, 30 ℃, 150 r / min, liquid volume 20%, cultivated for 12 hours to obtain seed liquid; inoculated the seed liquid with 4% inoculum in the enzyme-producing medium, 150 r / min, 25°C for 20 h, 10000 r / min Centrifuge for 5 minutes, and take the supernatant, which is the low-temperature amylase crude enzyme solution.

Embodiment 3

[0082] Embodiment 3, a kind of low-temperature amylase produced by the method for producing low-temperature amylase from the oceanic Yarrow yeast FA2-3 as described in embodiment 2, the character of described low-temperature amylase is: the minimum of low-temperature amylase The suitable temperature is 30°C, and it still has enzyme activity at 0°C; the optimum pH is 8.0. The enzyme has good thermal stability at 30°C, and still has 85.8% enzyme activity in 5 hours, and the enzyme is stable at pH 4.0-9.0; Ba 2+ , Ca 2+ , Mg 2+ and Sr 2+ Activation of enzymes, NH 4+ 、Cu 2+ 、Co 2+ There are varying degrees of inhibition of enzymes.

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Abstract

The present invention is a Yarrowia sp. FA2‑3 from the ocean, CGMCC No. 10377. Strain FA2‑3 is oblong, singly dispersed, and reproduces by budding. The colonies are white with neat edges. The growth temperature range of the strain is 15-35°C, and the optimum growth temperature is 30°C; the growth pH range is 5.0-10.0, and the optimum growth pH is 7.0; the strain can grow in the range of NaCl concentration of 0%-8%, and the optimum The NaCl concentration suitable for growth is 0.5%. The invention also discloses a method for producing low-temperature amylase by using bacterial strain FA2‑3 and a low-temperature amylase product obtained by the method. The low-temperature amylase has wide application prospects in the fields of feed, environmental protection and the like.

Description

technical field [0001] The present invention relates to a kind of microorganism, especially a kind of Yarrow yeast ( Yarrowia sp . ) FA2-3; the present invention also relates to the method and product for producing low-temperature amylase by the strain. Background technique [0002] Amylase is a general term for a group of enzymes that can catalyze the hydrolysis of starch into glucose, maltose and other oligosaccharides. It exists widely in animals, plants and microorganisms, and is widely used in papermaking, food, pharmaceutical and detergent industries. Ordinary amylase and high-temperature amylase have high temperature in the starch hydrolysis process, and the raw materials are easy to deteriorate, which restricts the further application of amylase. Cold temperature-enzyme refers to a class of enzymes that can effectively catalyze biochemical reactions at low temperatures. The optimum reaction temperature is 20-30°C lower than ordinary enzymes, and it has certain act...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12N9/30C12R1/645
CPCC12N9/242C12Y302/01001C12N1/145C12R2001/645
Inventor 吕明生王淑军李秋潼任伟房耀维刘姝焦豫良
Owner HUAIHAI INST OF TECH
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