Method for controlling melanophila picta pallas larvae through fusarium proliferatum carried on sclerodermus pupariae
A technology for Gitella poplar, layered Fusarium, applied in the directions of botanical equipment and methods, insecticides, animal repellants, etc., can solve the problems of difficult control, poor control effect, pesticide residues and the like, To achieve the effect of increasing larval mortality
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Embodiment 1
[0025] The method for the prevention and treatment of the larvae of Populus chinensis by carrying the Fusarium spp. of the present invention is located at the Karamay afforestation and emission reduction base in the southeast of Karamay City, Xinjiang. The geographical coordinates are: East longitude: 84 ° 59' 38.56 ", 45°28'1.34" north latitude. It is a high-density pure poplar forest, 95% of which are Russian poplar, mixed with other tree species such as elm, Populus euphratica, willow, etc. The spacing between plants is 1m, and the row spacing is 2m. The average insect population density per plant is 13-20 heads, and the rate of insect-bearing plants is 75% for verification. The specific steps are as follows:
[0026] a. Cultivation of entomogeneous fungi: inoculate Fusarium sp. on a PDA plate, cultivate at 28°C for 5 days, inoculate the purely cultured bacterial strains on the inclined plane of a 15mm×150mm PDA test tube, cultivate at 28°C for 9 days, and produce a large a...
Embodiment 2
[0030] The method for the prevention and treatment of Populus spp. with Fusarium spp. of the present invention is located in the Karamay afforestation and emission reduction base in the southeast of Karamay City, Xinjiang. The geographical coordinates are: East longitude: 84 ° 59' 38.56 ", At 45°28′1.34″ north latitude, it is a pure poplar forest with high density planting. 95% of the tree species are Russian poplar, mixed with other tree species such as elm, Populus euphratica, willow, etc. The spacing between plants is 1m and the row spacing is 2m. The average insect population density per plant is 13-20 heads, and the rate of insect-bearing plants is 75% for verification. The specific steps are as follows:
[0031] a. Cultivation of entomogeneous fungi: Inoculate Fusarium sp. on a PDA plate, cultivate at a temperature of 25°C for 6 days, inoculate the purely cultured bacterial strains on the inclined plane of a 15mm×150mm PDA test tube, cultivate at a temperature of 25°C for...
Embodiment 3
[0035] The method for the prevention and treatment of Populus spp. with Fusarium spp. of the present invention is located in the Karamay afforestation and emission reduction base in the southeast of Karamay City, Xinjiang. The geographical coordinates are: East longitude: 84 ° 59' 38.56 ", At 45°28′1.34″ north latitude, it is a pure poplar forest planted with high density. 95% of the tree species are Russian poplar, mixed with other tree species such as elm, Populus euphratica, willow, etc. The spacing between plants is 1m, and the row spacing is 2m. , there are 75% of strains for verification, the specific steps are as follows:
[0036] a. Cultivation of entomogenic fungi: inoculate Fusarium sp. on a PDA plate, cultivate at 23°C for 7 days, inoculate the purely cultured strains on the slope of a 15mm×150mm PDA test tube, and cultivate at 23°C for 13 days to produce a large amount of bacteria. sporulation;
[0037] b. Scleroderma infestation: Select healthy and active Sclerod...
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