24 results about "Populus euphratica" patented technology

The invention discloses paenibacillus FHY1 and application thereof, and belongs to the field of microorganisms. A strain of paenibacillus FHY1 is screened from Xinjiang populus euphratica juice, and experimental verification shows that a liquid culture of the paenibacillus FHY1 strain provided by the invention has a remarkable inhibition effect on magnaporthe oryzae, the bacteriostasis rate can reach 84%, and the inhibition rate on magnaporthe oryzae spore germination is 92%. And the rice growth can be promoted under the conditions of 150mM NaCl and 250mM NaCl. Meanwhile, the strain also has a good inhibition effect on pyricularia grisea, fusarium oxysporum, fusarium graminearum, sclerotinia sclerotiorum and colletotrichum gloeosporioides. Therefore, the invention provides a new strain with a good control effect on pathogenic fungi, and meanwhile, the growth and development of rice are remarkably promoted in a salt stress environment.

The invention discloses a populus euphratica PeXTH7 gene and application of the populus euphratica PeXTH7 gene in improving drought resistance of plants, and belongs to the technical field of gene engineering. The CDS sequence of the populus euphratica PeXTH7 gene is disclosed for the first time and is as shown in SEQ ID NO.1, and the coded amino acid sequence is as shown in SEQ ID NO.2; the effect of the populus euphratica PeXTH7 gene in improving the drought resistance of the plant is disclosed for the first time, the root length and lateral root density of the plant are remarkably improved through overexpression of the populus euphratica PeXTH7 gene, and the drought resistance of the plant is improved by adjusting the root structure of the plant and promoting root development; the populus euphratica PeXTH7 gene provided by the invention can be used as a candidate gene for breeding the drought-resistant new variety of the plant, and is of great significance for cultivating the drought-resistant new variety of the plant by adopting a genetic engineering method, accelerating the breeding process and reducing the breeding workload.

The present application relates to the intelligent mining direction of remote sensing data, and particularly relates to a poplar single tree crown segmentation method, device and equipment based on aerial image. The method comprises the following steps: acquiring a first multi-spectral unmanned aerial vehicle aerial image, and preprocessing the first multi-spectral unmanned aerial vehicle aerial image; inputting the first multi-spectral unmanned aerial vehicle aerial image into a crown top identification model based on a D-LinkNet network to obtain a poplar crown top seed point graph; using a watershed segmentation algorithm based on label control, taking the poplar crown top seed point graph and a first band of the first multi-spectral unmanned aerial vehicle aerial image as inputs to perform calculation, and segmenting a poplar single tree crown based on the first multi-spectral unmanned aerial vehicle aerial image; wherein the first band is an infrared band or a red edge band.

The invention relates to a salt-tolerant protein derived from populus euphratica and application of the salt-tolerant protein. The amino acid sequence of the salt-tolerant protein is as shown in SEQ ID No. 4. The salt-tolerant protein gene is transferred into a poplar, and after the poplar expresses the protein, the poplar can show the salt-tolerant characteristic, so that the protein can be expressed in a plant, and the plant can obtain the salt-tolerant characteristic.

The utility model discloses a populus euphratica seedling temperature control cultivation device, which belongs to the field of populus euphratica seedling cultivation and comprises a cultivation box, air ports are arranged on the front side wall and the rear side wall of the cultivation box, and a box door is mounted on the side wall of the cultivation box through hinges. A heating plate and a temperature sensor are mounted on the right side wall of the inner cavity of the cultivation box; through a heating plate, a fan, a temperature sensor and the like, the temperature sensor is set to be the optimal growth temperature value of populus euphratica seeds or seedlings, when the air temperature in an incubator is lower than the set value of the temperature sensor, the heating plate is started to heat air to the set temperature value, and when the air temperature is higher than the set value of the temperature sensor, the fan is started to blow air into the incubator; heat transfer and dissipation in the cultivation box are accelerated, the temperature is reduced to a set value, the problems that the temperature in an existing populus euphratica seedling cultivation device cannot be stably controlled, and seed germination and seedling growth are not facilitated are solved, and therefore the growth speed of populus euphratica seedlings can be increased.

The application discloses a method for assisting Populus euphratica seed germination and seedling growth in saline-alkali land, and the method is characterized in that a seedling making base is applied in a high-alkali environment to block the surface layer of saline-alkali, so that the Populus euphratica seed germinates and grows in the base, and a low-to-high saline-alkali gradient is formed from the center to the edge of the seedling making base, so that the early seedling of the Populus euphratica gradually adapts to the saline-alkali environment; when the root system of the seedling is completely developed and the seedling preliminarily has the stress resistance to the saline-alkali, the seedling penetrates the seedling making base to contact the saline-alkali soil in the natural environment, so that the transition of the life process of the Populus euphratica seed developing into a whole body is realized, the adverse effect of a large amount of saline-alkali left on the surface layer after the evaporation of the perennial large amount of river water irrigation on the germination of the Populus euphratica is avoided, and the survival rate of the seedling growth of the Populus euphratica seed is increased.

The invention discloses a populus euphratica cloned sub-plant water utilization strategy optimization method based on branch seepage rotation irrigation, and relates to the technical field of desert plant ecological restoration, the optimization method comprises the following specific steps: selecting a branch seepage rotation irrigation ecological restoration test area, dividing a root cutting area and digging a root cutting ditch, and adopting three irrigation modes for processing; determining a cloned child plant-mother plant pair as a research object in September after three years; synchronously collecting multiple types of samples, and sealing and storing at low temperature; treating the sample and measuring indexes; quantifying a water contribution proportion through a comparison method and a model, determining an optimal irrigation mode, and optimizing a water utilization strategy; by combining the multi-mode irrigation and isotope analysis technology, the water source of the populus euphratica cloned sub-plants is accurately analyzed, a traditional experience-driven mode is broken through, the water resource utilization efficiency is improved, meanwhile, the Bayesian model is utilized to quantify the water absorption difference of the cloned sub-plants of different tree ages, a theoretical basis is provided for formulating a differential irrigation scheme, and the method is suitable for popularization and application. And sustainable restoration of an ecological system is promoted.

The present application belongs to the technical field of agricultural compositions, and relates to an agricultural composition for preventing and treating populus euphratica wood lice. The present application provides an agricultural composition for preventing and treating populus euphratica wood lice, wherein the effective components are composed of fluopyram and echinacoside, and the mass ratio of fluopyram to echinacoside is 1:1-37.5. It is found through insecticidal effect verification that echinacoside has a synergistic effect on the chemical insecticidal component fluopyram, and the combination of the two can effectively reduce the effective component dosage of chemical pesticides, fully exert the efficacy of fluopyram, as much as possible reduce the probability of populus euphratica wood lice developing drug resistance, reduce environmental pollution, and improve the effect of fluopyram.

The present application relates to the technical field of poplar plant genetic engineering and stress resistance molecular breeding, and particularly relates to a Populus euphratica PeBCH1 gene, a recombinant vector and an application of a coded protein of the recombinant vector in salt tolerance of poplar, and the recombinant vector. The present application verifies that the Populus euphratica PeBCH1 gene participates in a response process of poplar to salt stress through a 84K poplar genetic transformation experiment, and has a role of regulating salt tolerance of poplar. Since the recombinant vector contains the Populus euphratica PeBCH1 gene, and the PeBCH1 protein is coded by the gene, therefore, the recombinant vector containing the Populus euphratica PeBCH1 gene and the coded PeBCH1 protein can be used to improve the salt tolerance of poplar. This lays a foundation for studying a response mechanism of poplar to salt stress, and provides a theoretical basis for cultivating a salt-tolerant poplar variety.

This invention discloses a strain of Bacillus subtilis FHY1 and its applications, belonging to the field of microbiology. The present invention obtained a strain of Bacillus subtilis FHY1 from the juice of Populus euphratica in Xinjiang. Experimental verification shows that the liquid culture of the Bacillus subtilis FHY1 strain provided by this invention has a significant inhibitory effect on rice blast fungus, with an inhibition rate of 84% and an inhibition rate of 92% on rice blast fungus spore germination. It can promote rice growth under conditions of 150mM NaCl and 250mM NaCl. Simultaneously, this strain also has good inhibitory effects on rice blast fungus, Fusarium oxysporum, Fusarium graminearum, Sclerotinia sclerotiorum, and Anthracnose fungus. Therefore, this invention provides a new strain with good control effect against pathogenic fungi, and significantly promotes rice growth and development under salt stress.

The application belongs to the technical field of plant genetic engineering, and particularly relates to a Populus euphratica PeAREB1 gene and application thereof in improving drought resistance of plants. The nucleic acid sequence of the Populus euphratica PeAREB1 gene is shown as SEQ ID NO. 1, and the amino acid sequence coded by the gene is shown as SEQ ID NO. 2. The gene is constructed into a plant expression vector, and is transformed into plants, so that the drought resistance of the plants can be improved, and the application prospect in breeding of plants with stress resistance is good.

The invention discloses a method for regulating and controlling propagation of populus euphratica and stability of rhizosphere microorganisms through branched seepage rotation irrigation, and relates to the technical field of ecological restoration. According to the method, by differentiating branched seepage rotation irrigation modes, precisely matching habitat requirements of sexual and clone propagation populus euphratica seedlings, and adopting a long-interval and gradually-water-increasing irrigation mode for the clone propagation seedlings, the propagation of populus euphratica and the stability of rhizosphere microorganisms are regulated and controlled; according to the method, soil salt leaching and nitrogen and phosphorus accumulation can be promoted, functional florae such as rhizosphere actinomycetes and thick-wall bacteria are activated, complex organic matter is degraded by secreting extracellular enzyme, the phosphorus dissolving capacity is enhanced, the nutrient obtaining efficiency of seedlings in a medium-high-salt environment is improved, a short-interval and high-water-quantity mode is adopted for sexual propagation seedlings, a low-salt and high-humidity habitat is created, and the survival rate of the seedlings is increased. Growth-promoting florae such as pseudomonas are enriched, and by secreting growth hormones and antagonistic substances, the planting capacity of the seedlings in a humid environment is enhanced, so that directional regulation and control of the two breeding modes are realized.

The invention belongs to the technical field of forest tree genetic breeding, and particularly relates to application of ethylene glycol bis (2-aminoethyl ether) tetraacetic acid in populus euphratica distant hybridization breeding. The invention provides an application of ethylene glycol bis (2-aminoethyl ether) tetraacetic acid in cross breeding of populus euphratica and populus euphratica. The ethylene glycol bis (2-aminoethyl ether) tetraacetic acid is used for overcoming distant hybridization obstacles of populus euphratica. Compared with the application of exogenous plant growth regulators such as 1-naphthylacetic acid and gibberellin A3, the application method provided by the invention has the advantages that the pollination success rate can be obviously improved, distant hybridization obstacles are overcome, the operation is simple and convenient, and the application method is suitable for being popularized in forestry breeding.

The application discloses a method for supplementally planting Populus euphratica and its companion species by drip irrigation combined with underground water, and steps are as follows: S1, determining a supplementally planting plot and a water source, and carrying out land preparation; S2, calculating a required Populus euphratica number, and determining a companion species type, a planting mode and a required number; S3, reasonably arranging seedling raising according to a supplementally planting time; S4, carrying out supplementally planting seedling transplanting and seed direct sowing; and S5, reasonably carrying out seedling period management.The method provided by the application has multi-effectiveness, can significantly improve a Populus euphratica forest seed update survival rate, simultaneously fully utilizes topographic advantages and residual flood storage of a previous year, improves water utilization efficiency, and plays a good water-saving role; the method is good in economic practicability, is simple in technical operation and easy to master, is good in material economy and practicability, greatly reduces an ecological forest protection and repair input cost, and is suitable for wide range of popularization and application.

This invention relates to the field of plant care technology and provides a poplar seedling cultivation system, comprising: a soil analysis module for analyzing soil information per unit area and evaluating the soil score of each unit area; a seed screening module for initial screening of poplar seeds and evaluating the sowing score of each seed; a seedling cultivation module for allocating poplar seeds with each sowing score to a unit area corresponding to the soil score, and providing seedling temperature and humidity conditions based on the sowing score; and a maintenance module for acquiring the height information of the poplar seedlings in real time, determining the growth level based on the height information, and setting control parameters for irrigation and nutrient units for each unit area based on the growth level. This invention achieves precise planting and personalized maintenance of poplar seedlings, helping to improve the growth efficiency and quality of poplar seedlings.

The application discloses a Populus euphratica rust detection and prevention method, which comprises the following steps: preparing a Zanthoxylum bungeanum extract by taking Zanthoxylum bungeanum branches and leaves; preparing a Sophora flavescens extract by taking dried Sophora flavescens roots; mixing and compounding the Zanthoxylum bungeanum extract, the Sophora flavescens extract, 15% triazole ketone wettable powder, calcium lignosulfonate, chitosan and urea to obtain a Populus euphratica rust prevention agent; and spraying the prepared Populus euphratica rust prevention agent on Populus euphratica. The Fv / Fm value of the test leaf is determined by using a chlorophyll fluorometer, statistical analysis is performed on the obtained Fv / Fm value, and the Fv / Fm value range of the healthy leaf and the diseased leaf is obtained, which is used for determining the health condition of the Populus euphratica according to the Fv / Fm value. The application utilizes the chlorophyll fluorescence characteristics of the Populus euphratica leaf, and provides a simple and effective detection method, which provides a certain basis for controlling the spread of the rust. The application also provides a Populus euphratica rust prevention method, and the prepared Populus euphratica rust prevention agent has excellent prevention effect and can effectively control the occurrence or spread of the rust.

The invention discloses an ecological restoration method for improving quality and efficiency of a declined artificial haloxylon ammodendron forest, and belongs to the technical field of desert vegetation restoration. According to the method, a comprehensive decline index is calculated by measuring multiple indexes such as the ratio of dead and dead trees in the haloxylon ammodendron forest, the canopy coverage loss rate of the haloxylon ammodendron forest, the shoot withering rate of the haloxylon ammodendron forest, the total vegetation coverage of the haloxylon ammodendron forest and the root exposure rate of the haloxylon ammodendron forest, and the haloxylon ammodendron forest is accurately divided into four grades of non-degradation, mild decline, moderate decline and severe decline. Then differentiated restoration is carried out according to different decline grades, wherein redundant branches are mainly pruned in light decline forests; thinning two-stage branches and cleaning secondary dead branches in the moderately declined forest; in the severe decline forest, stumping is carried out on the trunk withered plants. And the branches obtained by pruning or stumping are laid on the spot to form a sand barrier, so that resource recycling is realized. The problems that in the prior art, degradation grade division is fuzzy, repairing measures are homogenized, the cost for supplementing other sand barrier materials is high, and the environmental hidden danger is large are solved, precise diagnosis and targeted repairing of the declined haloxylon ammodendron forest are achieved, and the ecological function and stability of the forest stand are effectively improved.

The application belongs to the technical field of irrigation and relates to a Populus euphratica forest area division and rotation irrigation method, comprising the following steps: division into a first flooding irrigation area, a second flooding irrigation area and a third flooding irrigation area; comparison of the water inflow frequency value with a preset water inflow frequency interval; determination of the number of flooding irrigation times and the number of flooding irrigation days in the current year; and drainage irrigation. The application can dynamically select different flooding irrigation areas for irrigation according to the water inflow frequency value corresponding to the real-time flow of the main river channel, so that the first flooding irrigation area, the second flooding irrigation area and the far river area can obtain irrigation opportunities under different water inflow conditions, the far river area can be prevented from continuously degrading due to insufficient water distribution, the ecological water resources can be evenly distributed among different areas away from the river channel, the number of irrigation times and the number of irrigation days of each flooding irrigation area can be accurately determined in combination with the underground water depth data, and the overall restoration scale and ecological restoration effect of the Populus euphratica forest are improved.

The invention discloses a PeHB7 gene related to salt tolerance of poplar, a protein, a recombinant vector, a host cell and application, and relates to the technical field of plant molecular biology. The nucleotide sequence of the PeHB7 gene is as shown in SEQ ID NO. 1. The HD-Zip I subfamily gene PeHB7 gene related to salt tolerance is cloned from populus euphratica for the first time, the nucleotide sequence, the coded protein structure and the salt tolerance function of the gene PeHB7 gene are defined, poplar HD-Zip gene family research resources are enriched, and a key candidate gene is provided for analyzing a poplar salt tolerance molecular mechanism. The salt tolerance of the obtained transgenic plant can be remarkably improved under the high-salt condition through the ways of maintaining cell membrane integrity, reducing MDA and ROS accumulation, maintaining photosynthetic function stability and the like, and the growth state of the transgenic plant is far superior to that of a wild type. The PeHB7 gene and the related technology provided by the invention are simple and convenient to operate and high in efficiency, can be directly applied to salt-tolerant molecular breeding of the poplar and cultivation of a new salt-tolerant poplar variety, provide excellent germplasm for afforestation of the poplar in saline-alkali soil, and have theoretical significance and practical value.

The invention belongs to the technical field of transgenosis, and particularly relates to an agrobacterium tumefaciens-mediated populus euphratica callus genetic transformation system which comprises the following steps: step 1, activating strains; step 2, preparing an infection solution; step 3, generation of calluses; step 4, infection and co-culture; step 5, removing bacteria; step 6, screening and culturing; step 7, detecting positive seedlings by a PCR (Polymerase Chain Reaction) method; step 8, detecting a positive seedling by a Ruby method; and step 9, regeneration and transplanting are carried out, and the strain activation is to take an agrobacterium liquid containing a binary plant expression vector pCX-GUS-PeuCIPK1 preserved at-80 DEG C, place the agrobacterium liquid on ice and melt the agrobacterium liquid in a super clean bench. According to the method, the populus euphratica callus is used as a gene receptor material for agrobacterium tumefaciens mediation, so that a transgenic plant is obtained, the operation is simple, and a powerful technical support is constructed for applying a modern biotechnology to populus euphratica transgenic research.

The invention discloses an efficient tissue culture and regeneration system for populus euphratica. The efficient tissue culture and regeneration system comprises the following steps: step 1, selecting and disinfecting an explant; step 2, generation of calluses; 3, carrying out differentiation culture and bud generation; step 4, culturing strong buds; step 5, rooting culture; and step 6, transplanting of populus euphratica seedlings: selecting and disinfecting explants, namely selecting annual branches growing in the field in spring, cleaning dust on the surfaces of the branches, inserting the branches into water in bundles, standing indoors for two days, selecting tender complete wicker-shaped leaves at the front ends of the annual branches, disinfecting the tender complete wicker-shaped leaves with a 7.5% sodium hypochlorite solution for 5 minutes, and cleaning the tender complete wicker-shaped leaves with sterile water twice for 5 minutes each time. According to the method, regenerated plants obtained by tissue culture and rapid propagation of leaves are extremely high in regeneration efficiency, high in propagation coefficient, high in propagation speed and good in rooting effect, and the yellowing condition of populus euphratica is effectively reduced.

This invention discloses a callus induction culture medium and a method for constructing a regeneration system for Populus euphratica, belonging to the field of plant tissue culture technology. The Populus euphratica callus induction culture medium is prepared from the following raw materials: 1L of 1 / 2 MS medium, 0.1-1mg 6-BA, and 0.5-2mg NAA. The method for constructing a regeneration system using the callus induction culture medium includes the following steps: Populus euphratica seeds are disinfected and subjected to seed germination culture to obtain sterile seedlings. Cotyledons are then extracted and cultured in the callus induction culture medium for 30 days to obtain callus. The resulting callus is then differentiated and cultured in the callus differentiation culture medium to obtain adventitious buds. These adventitious buds are then rooted and induced in the rooting culture medium to obtain regenerated sterile seedlings. Stem segments with axillary buds are then cut and rooted for propagation to obtain Populus euphratica seedlings. This invention uses cotyledons from Populus euphratica seed germination as explants to provide a preliminary foundation for the preservation of superior Populus euphratica germplasm resources, the discovery of stress-resistant genes, and the establishment of a Populus euphratica genetic transformation system.

The application discloses a Populus euphratica clonal daughter plant water utilization strategy optimization method based on split infiltration wheel irrigation, and relates to the technical field of desert plant ecological restoration, and the specific steps of the optimization method are as follows: a split infiltration wheel irrigation ecological restoration test area is selected, a root breaking area is divided and a root breaking ditch is excavated, and three irrigation modes are adopted for treatment; after three years, a clonal daughter plant-mother plant pair is determined as a research object in September; multiple types of samples are synchronously collected and stored in a low temperature state; samples are treated and indexes are determined; the water contribution proportion is quantified by comparison and a model, the optimal irrigation mode is determined, and the water utilization strategy is optimized; by combining the multi-mode irrigation and the isotope analysis technology, the application accurately analyzes the water source of the Populus euphratica clonal daughter plant, breaks through the traditional experience-driven mode, improves the water resource utilization efficiency, simultaneously, the Bayesian model is used to quantize the water absorption difference of the clonal daughter plants of different ages, a theoretical basis is provided for formulating a differentiated irrigation scheme, and the sustainable restoration of the ecological system is promoted.

The invention discloses a method and system for estimating the aboveground biomass of populus euphratica based on a backpack type laser radar, and belongs to the technical field of machine algorithms. The invention aims to solve the problem that the existing remote sensing technology cannot perform high-precision single-tree segmentation and accurate biomass measurement and calculation on a small-range populus euphratica forest. According to the technical scheme, the method is characterized in that forest three-dimensional point cloud data are collected through a backpack type laser radar; preprocessing the point clouds to separate vegetation point clouds; carrying out populus euphratica individual tree segmentation on the point cloud based on a comparison shortest path algorithm; extracting tree height, diameter at breast height and crown breadth structure parameters of the single tree; estimating a trunk volume by using a different-speed growth model constructed based on the tree height and the diameter at breast height, and estimating a branch volume based on the crown breadth; and finally calculating the above-ground biomass of the single tree by combining the wood density, and calculating the total biomass of the forest stand. The method is mainly used for realizing non-destructive and high-precision evaluation of the growth condition of the populus euphratica in a small range.