Strain of sickle mycete and process for preparing ginseng saponin Rh2 thereof

A fusarium mold and ginsenoside technology, applied in the application field of preparing ginsenoside Rh2, can solve the problems of low substrate concentration and insufficient product yield, and achieve the effects of convenient preparation, low cost and low price

Inactive Publication Date: 2006-06-28
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the substrate concentration of the reaction is low, and the product yield is not high enough.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Slant culture: Caspian's agar medium was used. The strains were inoculated on the slant medium, and cultured at a constant temperature of 30°C for about 3 days.

[0023] Enzyme production culture: adopt Cha's liquid culture medium, 100ml shake bottle liquid volume 20ml, after sterilization, insert the culture solution that has been cultivated in the shake tube for 18 hours in advance as seeds, inoculum size 10% (v / v), culture The temperature is 30°C, the rotation speed of the shaker is 160r / min, and the shake flask is cultivated under the above conditions for 3 days, and the enzyme production can reach 48.8U / L fermentation broth.

[0024] The enzyme activity unit (U) is defined as: under the conditions of 40°C and pH 5.0, the amount of enzyme required to generate 1 μmol Rh2 per hour is defined as an enzyme activity unit (U).

Embodiment 2

[0026] The slant culture was the same as in Example 1. The culture solution that has been pre-cultivated for 18 hours at 30°C and 160r / min was used as a seed, and was transferred to a 100-ml shaker flask with 20ml of Cha's medium at an inoculum size of 10% (v / v). The culture temperature was 30°C and the shaker speed was 160r / min, and the culture was cultured for 24 hours, and 0.5 mg / ml total ginsenosides were added to induce enzyme production, and the culture was continued for 48 hours, and the enzyme production was doubled.

Embodiment 3

[0028] Seed culture is identical with embodiment 2. Put 200ml of Chaspearl's liquid culture medium into several 1L large shaker flasks, after high-temperature sterilization, add 8ml of seed solution pre-cultivated for 18 hours, at a culture temperature of 30°C and a shaker speed of 160r / min After 3 days of under-cultivation, the enzyme production reached 30.9U / L fermentation broth.

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PUM

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Abstract

The invention discloses fusarium proliferatum and the method used to manufacture ginsenoside Rh2. It belongs to fusarium proliferatum ECU2042 CGMCC No.1495. The invention catalyzes and hydrolyzes relative common ginsenoside to manufacture rare saponin, and use the fusarium proliferatum to manufacture ginsenoside Rh2. It cost is relative low. And transformation ratio can reach above 60%. The formed saponin purity is not less than 95%.

Description

technical field [0001] The present invention relates to a fusarium fungus and its application, in particular to a fusarium fungus producing specific β-glucoside hydrolase and its application method in preparing ginsenoside Rh2. technical background [0002] Ginseng is a precious traditional Chinese medicine in my country. It has many pharmacological and biological activities such as nourishing, strengthening, anti-fatigue, and anti-tumor. Studies have shown that ginsenoside is its main active ingredient, and more than 40 kinds of ginsenosides have been isolated and identified. All kinds of ginsenosides have their unique curative effects, among which ginsenoside Rh2 has inhibitory effect on the proliferation of various cancer cells, and is an anticancer drug with great development potential. However, the content of ginsenoside Rh2 in ginseng is extremely small, so it is very difficult to extract directly. Relatively speaking, the extraction and preparation of ginsenoside Rg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P1/02C12P33/00C12R1/77
Inventor 许建和苏金环
Owner EAST CHINA UNIV OF SCI & TECH
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