The invention discloses bacillus licheniformis 201 for high effectively preventing banana wilt, and the preservation number of the bacillus licheniformis 201 is CCTCCM207092. Bacterial colony on beef extract-peptone medium is greyish-white, round, flat and lackluster, the edge of the bacterial colony is in order, the diameter of the bacterial colony is generally 2 to 3 millimeters, bacteria are of straight rod shape, the size of the bacteria is 2.06* 0.70 micrometers, and spore is of elliptical shape. Simultaneously, the invention also discloses a process for preparation and usage. The bacillus licheniformis 201 is capable of strongly preventing fusarium oxysporum and has wide antimicrobial spectrum, which is capable of performing antagonistic action on various pathogenic fungi, such as fusarium oxysporum, fusarium oxysporum f. sp. niveum, fusarium oxysporum f. sp. lycopersici, gloeosporium musarum, colletotrichum gloeosporioides, maize curvularia leaf spot fungus, verticillium wilt pathogen, wheat scab pathogen and the like, furthermore, the invention is high effective in prevention, good in environmental security and good in the prospect of development and application.