Bacillus subtilis 203 for highly effective preventing and curing banana wilt and application thereof

A technology for Bacillus subtilis and banana Fusarium wilt, applied in the application, bacteria, fungicides and other directions, can solve problems such as blank and weak technology, and achieve the effects of easy preservation, fast reproduction, and good development and application prospects.

Inactive Publication Date: 2008-05-28
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Isolation and screening from banana plants to obtain biocontrol bacterial strains with antagonistic effects on Fusarium wilt

Method used

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  • Bacillus subtilis 203 for highly effective preventing and curing banana wilt and application thereof
  • Bacillus subtilis 203 for highly effective preventing and curing banana wilt and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Plate Antagonism Experiment of Bacillus subtilis 203 on Banana Fusarium wilt No. 4 Race FOC4

[0021] Bacillus subtilis 203 was obtained by face-to-face screening on a plate, and the screening method belongs to a routine test method in the art. In the center of the potato sucrose medium PDA plate, insert a piece of FOC4 mycelium block of Fusarium wilt No. 4 race with a diameter of 5 mm, and then put a sterilized filter paper sheet with a diameter of 5 mm on the triangle of the equidistant FOC4 mycelium block, each piece Insert 10 μL of Bacillus subtilis 203 culture solution on the filter paper, take the plate without Bacillus licheniformis 202 culture solution as the control, repeat three times, culture in the incubator at 25-28°C for 5-7 days, measure the diameter of the inhibition zone, and use the attached Figure 1 shows that Bacillus subtilis 203 significantly inhibits the growth of Fusarium wilt of banana.

Embodiment 2

[0022] Example 2 Bacillus subtilis 203 prevents banana plant main root and vascular bundle from browning or rot experiment

[0023] Select Brazilian banana seedlings with a plant height of about 15 cm, and carry out 3 different treatments: (1) clean water healthy plant control: the roots of banana seedlings are soaked in sterile water and then directly transplanted in a nutrient bowl to grow; (2) the roots of banana seedlings are soaked in sterile water. After 30 minutes in the Bacillus subtilis 203 culture solution, transplant it into the nutrient bowl, and then pour 20mL of FOC4 spore suspension into the nutrient bowl soil; (3) Incidence control: root irrigation of banana seedlings inoculated with 20mL of FOC4 spores Suspension, not inoculated with Bacillus subtilis 203 culture solution. For the above treatments, there were 20 plants in each treatment group. Afterwards, culture at 26°C, investigate the incidence rate and disease index at 10, 20, and 30 days after inoculatio...

Embodiment 3

[0024] Example 3 Antagonism of Bacillus subtilis 203 to various pathogenic bacteria

[0025] The antagonism of Bacillus subtilis 203 to 7 pathogenic bacteria including tomato fusarium wilt, Curvularia cornis leaf spot, Glue sporotrichum anthracnose, cotton wilt, cotton Verticillium wilt, wheat scab, and watermelon wilt was tested by plate confrontation method Effect, insert a target mycelium block with a diameter of 5mm in the center of the PDA plate, and then put a sterilized filter paper sheet with a diameter of 5mm on the triangle of the equidistant FOC4 mycelium block, and insert Bacillus subtilis 203 on each filter paper sheet for cultivation 10 μL of liquid solution, and a plate without Bacillus licheniformis 202 culture solution as a control, cultivated in a 28°C incubator for 5 to 7 days, and measured the diameter of the inhibition zone. Bacillus subtilis 203 has a wide antibacterial spectrum and has antagonistic effects on various pathogenic fungi such as Fusarium wil...

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Abstract

The invention discloses bacillus subtils 203 for high effectively preventing banana wilt, and the preservation number of the bacillus subtils 203 is CCTCCM207094. The invention simultaneously discloses a process for preparation and usage. The bacillus subtils 203 are obtained after being selected by being cultured face to face on a plate and doing pot experiment, which which is capable of effectively preventing the growth of fusarium oxysporum and has wide antimicrobial spectrum. The invention is also capable of performing antagonistic action on various pathogenic fungi, such as cotton-wilt fusarium, verticillium wilt pathogen, fusarium oxysporum f. sp. niveum, fusarium oxysporum f. sp. lycopersici, curvulavia lunata or colletotrichum musae and the like, which is good in environmental security and good in prospect of development and application.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to an endogenous biocontrol bacterial strain for preventing and treating banana wilt, in particular to a bacillus subtilis 203 for efficiently preventing and controlling banana wilt and its application. Background technique [0002] Banana Fusarium wilt is a devastating disease caused by the infection of Fusarium oxysporum f.sp.cubense, and it is a devastating soil-borne disease of banana production. The pathogen invades from the injured rhizome, spreads to the pseudostem and leaves through the vascular bundle, and blocks the xylem vessel until the plant withers and dies. Fusarium wilt of banana is a soil-inhabiting fungus, and its chlamydospores can survive in the soil for 8 to 10 years. to resume production. Banana Fusarium wilt is distributed in many counties and cities in the banana production area of ​​the Pearl River Delta, and it has developed rapidly in recent years. The r...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125
Inventor 王振中孙正祥纪春艳
Owner SOUTH CHINA AGRI UNIV
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