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Trichoderma asperellum TZ312, application thereof, volatile metabolite thereof and application of volatile metabolite

A technology of Trichoderma aculeatus and metabolites, applied in the field of microorganisms, can solve problems such as poor control effect of banana fusarium wilt, and achieve the effects of strong rhizosphere colonization ability, reduced incidence and broad antibacterial spectrum.

Active Publication Date: 2020-11-06
INST OF PLANT PROTECTION GUANGXI ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defect that the existing control effect of banana wilt is poor, the present invention provides Trichoderma aculeatus TZ312 and its application, the volatile metabolite of Trichoderma aculeatus TZ312 and its application , the Trichoderma aculeatus TZ312 can inhibit various soil-borne diseases such as banana wilt, and the volatile metabolites of Trichoderma aculeatus TZ312 can significantly inhibit the occurrence of storage diseases

Method used

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  • Trichoderma asperellum TZ312, application thereof, volatile metabolite thereof and application of volatile metabolite
  • Trichoderma asperellum TZ312, application thereof, volatile metabolite thereof and application of volatile metabolite
  • Trichoderma asperellum TZ312, application thereof, volatile metabolite thereof and application of volatile metabolite

Examples

Experimental program
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Effect test

Embodiment 1

[0048] 1. Strain isolation

[0049] The samples of 5 points in the same banana orchard, the soil samples near the roots (shaking the roots and shaking off) were crushed, passed through a 2mm sterilizing sieve (10 mesh) and then mixed. The soil mixture was transferred to a sterilized Erlenmeyer flask, and a sterile 0.85% NaCl solution was added at a ratio of 1:9 (v / v) (simultaneously put the root segments with soil on the surface into the flask). One banana garden was repeated three times. All soil suspensions were shaken on a rotary shaker at 150 rpm for 30 minutes at 25°C and then allowed to stand for 5 minutes. With 0.85% NaCl solution, the obtained soil suspension is serially diluted 10 times, and the dilution factor is 10 -2 、10 -3 0.2ml solution of soil suspension into Martinmedium medium (10g glucose, 5g peptone, 1g KH 2 PO 4 , 3.3ml 1% (w / v) rose bengal solution, 0.5gMgSO 4 .7H 2 O, 15g agar, 1000ml dH 2 (2, 100 mg streptomycin), spread the rod and mix well, rep...

Embodiment 2

[0067]Example 2 Trichoderma aculeatus TZ312 inhibits the mycelial growth of Fusarium wilt of banana

[0068] Method: Trichoderma aculeatus TZ312 was inoculated on a 9cm PDA plate in the four directions of southeast, northwest, and 0.5cm away from the edge of the plate, the center of the PDA plate was inoculated with Fusarium wilt, and placed in an incubator at 25°C for 6 days. The blank control was not inoculated with TZ312. When the colony growth diameter of Fusarium wilt of the blank control reaches the edge of the dish, observe the confrontation situation of the treatment plate.

[0069] Result: if image 3 As shown, on the standoff tablet ( image 3 On the right panel), Trichoderma aculeatus TZ312 grows rapidly, and its dark green spores cover the hyphae of Fusarium wilt fungus, suspected to be parasitic on it, and strongly inhibit the growth of Fusarium wilt fungus mycelia.

Embodiment 3

[0070] Embodiment 3 Trichoderma aculeatus TZ312 inhibits the occurrence of banana wilt

[0071] Method: inoculate the banana Fusarium wilt bacteria (concentration of 1.0×10 6 individual / g); after 1d, Trichoderma aculeatus TZ312 bacterial strain (concentration 1.0×10 6 Individual / g), the banana tissue culture seedlings with a height of about 30cm were planted after 2 days of shading treatment, as a treatment group. Before planting, banana tissue-cultured seedlings were soaked in Trichoderma aculeatus TZ312 bacterial solution for 30 minutes.

[0072] Blank control 1 (CK1) was inoculated with the bacterial solution of Fusarium wilt of banana, blank control 2 (CK2) was not inoculated with any bacterial solution, and other treatments were the same as those of the treatment group.

[0073] Nine banana tissue culture seedlings were planted for each bacterial strain. Cultivate in a greenhouse at 28-32°C for 45d-60d. Investigate the condition index when CK1 is obviously onset.

[...

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Abstract

The invention provides trichoderma asperellum TZ312, application thereof, a volatile metabolite thereof and application of the volatile metabolite, and relates to the technical field of microorganisms. The trichoderma asperellum TZ312 is preserved in Guangdong Microbiological Culture Collection Center and has a preservation number of GDMCC NO:60253. The trichoderma asperellum TZ312 is capable of completely inhibiting and covering growth of hyphae of banana wilt disease germs on a panel; and the trichoderma asperellum TZ312 is capable of obviously decreasing the occurrence rate of a banana wiltdisease in a greenhouse pot experiment, and the prevention effect is 65% or above. The trichoderma asperellum TZ312 is further capable of inhibiting fusarium oxysporum f.sp.cucumerinum, botrytis cinerea, botrytis cinerea, fusarium solani, fusarium proliferatum, magnaporthe oryzae, phomopsis sp., pestalotiopsis sp., botryosphaeria dothidea or colletotrichum gloeosporioides and is wide in antibacterial spectrum. The volatile metabolite of the trichoderma asperellum TZ312 can be used for inhibiting preservation diseases.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a strain of Trichoderma aculeatus TZ312 and its application, a volatile metabolite of Trichoderma aculeatus TZ312 and its application. Background technique [0002] Banana is one of the most important food crops in the world. In my country, banana is an important tropical and subtropical fruit with remarkable economic benefits and is one of the main industries for farmers to get rid of poverty and become rich. Banana Fusarium wilt is known as "banana cancer". The conidia of the pathogen Fusarium oxysporium f.sp.cubense can spread through running water and agricultural tools, and can spread in the form of chlamydospores Soil survives for decades. Infested bananas have no commercial fruits in the current or next generation, and the infected land cannot grow bananas for several years. Since the first discovery of banana fusarium wilt caused by physiological race No. 4 in Panyu D...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/38A01N35/02A01N31/08A01N27/00A01P3/00A23B7/144A23B7/154C12P5/02C12P7/22C12P7/26C12R1/885
CPCA01N35/02A01N31/08A01N27/00A23B7/144A23B7/154C12P5/02C12P7/22C12P7/26C12R2001/885C12N1/145
Inventor 黄穗萍莫贱友李其利郭堂勋唐利华
Owner INST OF PLANT PROTECTION GUANGXI ACADEMY OF AGRI SCI
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