35 results about "Germ tube" patented technology

The invention discloses a beauveria bassiana microcapsule formulation which is prepared by taking suspension prepared from beauveria bassiana conidia, kaolin and water as a capsule core, taking sodium alginate as a capsule wall, dispersing in a dispersing agent of n-hexadecane, corn oil and lecithin with different proportions, crosslinking through calcium ions, and solidifying microcapsules to enveloped fine particles with partical diameter being smaller than 100 Mu m. In the germination process of the beauveria bassiana conidia, a germ tube can perforate through the envelop of the capsule wall. The microcapsule formulation is also added with a conidium germination nutrient solution which increases the germination percentage and the germination speed of the conidia. The beauveria bassiana microcapsule formulation can effectively prevent the influences bad environments such as external ultraviolet rays and drying from the conidia, achieves high germination percentage under the influences of the same temperature, humidity and ultraviolet rays, and has good control effect to agriculture and forestry pests; and meanwhile the beauveria bassiana microcapsule formulation has simple production process and is easy for mass production.

The invention relates to a single spore isolation and purification method of verticillium dahliae. The method mainly comprises the following steps: an agar medium cylinder is taken from a water agar plate by the use of a glass capillary with diameter being 1mm and is picked onto a sterilized glass slide with a sterilization needle; a verticillium dahliae strain is activated in a PDA solid medium and the activated strain is cultured in a Czapek-Dox liquid medium; spore concentration is counted under a microscope; and the spore concentration is adjusted to 102-106 cfu to prepare a spore suspension; 1 microlitre of the spore suspension is sucked up and dripped onto the surface of the water agar cylinder on the glass slide; the glass slide is moved into a culture dish for constant temperature incubation at 25 DEG C; the glass slide is detected under the microscope, and the cylinder containing a single conidiospore which has germinated germ tubes is transferred onto a PDA plate to be cultured at 25 DEG C so as to finish single spore isolation. The method is simple to operate, can be implemented by general operation staff and is suitable for mass separation. By the method, separation success rate is high. The method is also suitable for production of other fungi of microconidia.

The invention discloses a Pseudomonas maltophilia stain QBA-5 with an effect of inhibiting Botrytis cinerea and application thereof. The strain QBA-5 obtained by the invention through screening is named as Pseudomonas maltophilia and is preserved in China Center for Type Culture Collection, with the preservation number CCTCC M 2015403. Specifically through experiments to Botrytis cinerea spores, germ tube elongation and detached leaves, the strain QBA-5 obtained by the invention is proved to have the effect of inhibiting Botrytis cinerea, can be prepared into bio-control agent for preventing and controlling Botrytis cinerea, and has a good market application prospect.

The invention belongs to the technical field of organic fertilizers and relates to a frass organic fertilizer for restraining broomrape, and a preparation method and a usage thereof. The frass organicfertilizer is prepared from Hermetia illucens frass and methyl alcohol. The frass organic fertilizer is an extract of the Hermetia illucens frass in the methyl alcohol and is prepared by the method:the Hermetia illucens frass is smashed and then mixed with a methyl alcohol solution by vibration, the extract is obtained through freeze drying of a supernate obtained by ultrasonic separation, and an addition amount of the Hermetia illucens frass is 1g Hermetia illucens frass/9 mL methyl alcohol; and Stratiomyidae frass can be used for restraining germination of the weed broomrape, growth of germ tubes of the weed broomrape and germination and parasitism of Orobanche Cumana. According to the fertilizer, through joint effects of the Hermetia illucens frass and the methyl alcohol, germinationof the broomrape and growth of the broomrape germ tubes can be effectively restrained, the germination and parasitism of Orobanche Cumana can also be restrained, nutrient losses in a plant root systemcan be prevented, a plant death rate is reduced, and the yield and quality of plants are improved.

The invention relates to a streptomyces mediolani ZW-1 bacterial strain, and a bacteriostatic application of fermentation broth thereof. Inhibited bacteria comprise wheat fusarium nivale, wheat fusarium graminearum, alternaria leaf spot, sclerotinia sclerotiorum, spinach altemaria solania, helminthosporium carbonum, watermelon fusarium wilt or cotton fusarium wilt. The streptomyces mediolani ZW-1 bacterial strain and the fermentation broth thereof can inhibit the growth of plant pathogenic fungal hyphae, lead to enlargement of cell walls of the pathogenic fungal hyphae and concentration of protoplasts, can inhibit conidial germination and germ tube growth of the spinach altemaria solania, have good bacteriostatic effects, and have good development and application prospects for biological prevention and control of plant diseases.

Xanthochymol and garcinol, isoprenylated benzophenones purified from Garcinia xanthochymus fruits, showed multiple activities against fungal biofilms. Both compounds effectively prevented emergence of fungal germ tubes and were also cytostatic, with MICs of 1 to 3 ΞM. The compounds therefore inhibited development of hyphae and subsequent biofilm maturation. Xanthochymol treatment of developing and mature biofilms induced cell death. In early biofilm development, killing had the characteristics of apoptosis. These activities resulted in failure of biofilm maturation and hyphal death in mature biofilms. In mature biofilms, xanthochymol and garcinol caused the death of biofilm hyphae, with 50% effective concentrations (EC₅₀s) of 30 to 50 μM. Additionally, xanthochymol-mediated killing was complementary with fluconazole against mature biofilms, reducing the fluconazole EC₅₀ from greater than 1,024 μg per ml to 13 μg per ml.

The invention discloses a hermaphrodite laver dihaploid colony building method. The method comprises taking hermaphrodite laver thalluses (gametophytes) with obvious color difference as cross parents, carrying out mixed cultivation on father and mother thalluses with antheridiums, collecting carpospores, selecting the carpospores with germ tubes, carrying out single culture to obtain protonema (sporophore), carrying out induction to obtain conchospore of the protonema, carrying out culture to obtain macroscopic F1 thalluses, wherein if 90% or more of the thalluses contain amphiphilic color chimeras, the thalluses are used for building of a dihaploid colony, separating all color blocks of the four-color block chimera, acquiring monospores or single isolated cells of each one of the color blocks, carrying out culture to obtain macroscopic thalluses, carrying out single thallus culture and autofertilization to obtain pure thallus of each one of color blocks of the four-color block chimera and building a dihaploid colony. The method solves problems of hermaphrodite laver dihaploid colony building and lays the foundation of genetic linkage map construction.

The invention discloses a Sporobolomyces huaxiensis Liu, whose fungus characters are: seperating tea blister blight by spore shooting method, besidiospore swelling to 20-25x6.5-8 mum with 3 separation after absorbed water observed by microscope, bearing 4 germ tubes, breeding rod shaped microspore directly on sterigma which one end is bit slim with length of 1.2 mum. Mature microspore is (6-)7.2-7.8(-9)x1.2-1.5(1.8) mum. The microspore breed by means of germinating easily to disperse. The basidiospore ejected on glass slide after induced tea blister blight besidiospore microcycle to produce spore, water swelling, breeding rod shaped microspore directly on sterigma. The invention also discloses the use thereof for irritating seed germinating and increasing amount of Lovastatin of red aspergillus ferment products.

The invention discloses a strain of euonymus japonicus endophytic bacterium bacillus methylotrophicus HYEB 5-6 which was preserved in China Center for Type Culture Collection on Dec 8th, 2015, and the preservation serial number is CCTCC No: M2015728. The bacillus methylotrophicus HYEB 5-6 is an euonymus japonicus endophytic bacterium and can delay germination of euonymus japonicus anthrax conidium, inhibit extension of a germ tube, inhibit germination of the conidium, induce hypha to generate an expanded body and cause malformation of the hypha; it is proved by inoculation experiment results that after HYEB 5-6 thallus is sprayed, the index of decease infection of euonymus japonicus anthracnose is remarkably inhibited, and scabs of euonymus japonicus anthracnose are reduced; the HYEB 5-6 strain has a certain antagonistic effect on euonymus japonicus colletotrichum, alternaria spp, cytospora chrysosperma, higgins dish bacteria, magnaporthe oryzae, rhizoctonia, horse chestnut fusicoccin and fusarium solani.

The invention discloses a method for controlling rice blast of rice, and relates to the technical field of biological control of rice pest and disease damage. The preparation method comprises the steps of gradient diluent preparation, NA solid culture medium preparation, bacillus separation, PDA culture medium preparation, spore solution preparation and rice colonization. Accordingly, bacilli obtained through separation can secrete an antibacterial material to inhibit pathogenic bacteria growth and induce a plant defence system to resist pathogenic bacteria invasion, supplied experimental strains have the significant inhibiting effect on germination of magaporthe grisea conidia, extension of germ tubes and formation of appressoria, the strain active substances have the high inhibiting effect on magaporthe grisea, and the magaporthe grisea mycelial growth inhibition rate reaches 80% or above; by means of silicon addition treatment, the rice seedling rice blast morbidity and disease index can be significantly lowered, the morbidity decreasing range can reach 30% or above, and the disease index is lowered by 40% or above. The resistance of the rice blast and the silicon content of different organs can be significantly increased through silicon treatment.

The invention discloses a Weissella fusion MP4 strain with an antibacterial effect and an application of the Weissella fusion MP4 strain. According to the invention, the MP4 strain with an inhibitingeffect on plant wilt caused by botrytis cinerea and fusarium oxysporum is obtained through screening; the strain is specifically classified and named as Wissella confuse MP4, is preserved in China Center for Type Culture Collection, and has a preservation number of CCTCC NO: M 2019701. According to the invention, experiments on botrytis cinerea and fusarium oxysporum spores, germ tube elongation and in vitro leaves are carried out; the MP4 strain has a high inhibition effect on botrytis cinerea and fusarium oxysporum, can be prepared into a biocontrol agent for preventing and treating botrytiscinerea and fusarium oxysporum, and has a good market application prospect.

The invention relates to a biological control method for rice blast and relates to the technical field of biological control of rice pests and diseases. The preparation method comprises preparing a gradient diluent, preparing a NA solid medium, separating bacilli, preparing an LB liquid culture solution, preparing a crude extract protein liquid and carrying out rice colonization. The isolated bacilli can secrete antibacterial substances to inhibit the growth of pathogenic bacteria and induce the plant defense system to resist invasion of pathogenic bacteria. The tested strain has remarkable effects of inhibiting the germination of the magaporthe grisea conidiophores, the prolongation of germ tubes and the formation of appressorium. The active substances of the strain have a strong inhibitory effect on magaporthe grisea and a magaporthe grisea mycelium growth inhibition rate of 80% or more.

The invention discloses a preparation method of a microbial pesticide for controlling rice blast, and relates to the technical field of biological control of rice diseases and pest insects; the preparation method includes the steps: preparation of a gradient dilution liquid, preparation of an NA solid culture medium, bacillus separation, preparation of a PDA culture medium, and preparation of a spore liquid. Bacillus obtained from isolation can secrete antibacterial substances to inhibit pathogenic bacteria growth, and induces a plant defense system against pathogenic bacteria invasion; a tested strain has a significant inhibitory effect on germination of magnaporthe grisea conidiospores, elongation of germ tubes and appressorium formation; the strain active substances have a relatively strong role in inhibiting magnaporthe grisea, and the magnaporthe grisea mycelium growth inhibition rate reaches 80% or more.

The invention discloses a biological prevention and control technology for rice blast, and relates to the technical field of biological prevention and control of rice diseases and pests. The preparation method includes the steps of preparing a gradient diluent, preparing an NA solid culture medium, separating bacilli, preparing a PDA culture medium, preparing a spore solution, preparing extracts of murraya paniculata leaves and planting the rice. The bacilli separated according to the method can secrete antibiotic substances to prohibit growth of pathogenic bacteria and entice a plant defencesystem to resist invasion of pathogenic bacteria, and supplied testing bacterial strains can achieve an obvious prohibition effect on germination of mitogenetic spores, extension of germ tubes and formation of appressorium of the rice blast bacteria. The active substances in the bacterial strains have a great prohibition effect on the rice blast bacteria, and the prohibition rate to the mycelial growth of the rice blast bacteria reaches 80% or above. The extracts of the murraya paniculata leaves have a capability of resisting the pathogenic bacteria, insect antifeedant activity and a certain prohibition effect on the rice blast pathogenic bacteria, wherein the callus extracts have excellent bacteriostatic activity, and the bacteriostatic activity to the rice blast bacteria reaches 90% or above.

The invention relates to the technical field of pest control, particularly a method for controlling early blight of greenhouse tomatoes by using an ultraviolet lamp. The invention aims to provide a method for producing pollution-free vegetables according to the actions of control of wavelength radiation dose on tomato early blight spore germination, germ tube extension and hypha growth inhibition. The ultraviolet lamp has great destructive power on deoxyriboneucleic acid (DNA) and ribonucleic acid (RNA) of pathogenic bacteria, and enables the pathogenic bacteria to lose survivability and fertility, thereby destroying the pathogenic bacteria. The method can implement nucleic acid mutation, replication inhibition, transcription blockade and protein synthesis, or can generate free radicals capable of causing photoionization, thereby resulting in cell death and further implementing sterilization. The sterilization efficiency is up to 99%; and the method has the advantages of favorable sterilization effect and high acting speed.

The present invention provides a Bacillus velezensis strain HMQAU19044, which was deposited in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on January 19, 2020, with a deposit registration number of CGMCC No.19420. Bacillus velezensis (Bacillus velezensis) strain HMQAU19044 provided by the present invention has significant control effect to cucumber downy mildew, and the research of embodiment shows, bacterial strain HMQAU19044 fermentation filtrate has obvious inhibition to the release of cucumber downy mildew zoospore Effect; the fermentation liquid of strain HMQAU19044 has obvious inhibitory effect on the release of zoospores of cucumber downy mildew; To a certain extent, the lesion expansion of the infected cucumber leaves is inhibited; the fermentation filtrate and fermentation liquid of the strain HMQAU19044 of the present invention have a good control effect on cucumber downy mildew.

Phakopsora pachyrhizi, the fungal pathogen that causes Asian soybean rust, has the potential to cause significant losses in soybean yield in many production regions of the U.S. To assist the development of new modes of soybean resistance to fungal infection, peptides were identified from combinatorial phage-display peptide libraries which inhibit germ tube growth from urediniospores of P. pachyrhizi. Two peptides, Sp2 and Sp39, were identified that inhibit germ tube development when displayed as fusions with the coat protein of M13 phage or as fusions with maize cytokinin oxidase/dehydrogenase (ZmCKX1). These peptides may be used in vitro to help control fungal infection. These peptides may also be expressed as transgenes in a plant to help the resulting transgenic plant defend against the fungi

The invention discloses a rice blast prevention and cure method, and relates to the technical field of biological prevention and cure of rice diseases and pests. A preparation method of the rice blastprevention and cure method comprises the steps of gradient dilution solution preparation, NA solid medium preparation, bacillus separation, PDA medium preparation, spore solution preparation and riceplanting. According to the rice blast prevention and cure method, bacilli obtained through separation can secret antibacterial material to inhibit growth of pathogenic bacteria and induce plant defense systems to defend against invasion of the pathogenic bacteria; tested bacterial strains have significant inhibition action on germination of conidia of magnaporthe grisea, germ tube extending and appressorium forming; active substances of the bacterial strains have strong inhibition action on the magnaporthe grisea, and the inhibition rate of growth of magnaporthe grisea hyphae reaches 80% or above.

The invention provides the application of verapamil hydrochloride in the preparation of fungicides for preventing and treating plant diseases caused by plant pathogenic bacteria. The present invention proves that verapamil hydrochloride has the effect on tomato Botrytis cinerea and Fusarium oxysporum through experiments on mycelium growth, spore germination and germ tube elongation of tomato Botrytis cinerea and Fusarium oxysporum. The tomato specialized type has strong inhibitory effect, and it can be prepared as a fungicide for preventing and treating botrytis cinerea and plant diseases caused by Fusarium oxysporum. Traditional pesticides pollute the environment with high residues and threaten food safety. The use of a large number of pesticides also leads to the enhancement of the resistance of pathogenic bacteria. As a fungicide, verapamil hydrochloride has the advantages of high efficiency and low toxicity. It is suitable for the requirements of chemical control of plant diseases and can ensure the high quality of agricultural products and fruits and vegetables. Development requirements, with broad research and market prospects.