10 results about "Germ tube" patented technology

The invention belongs to the technical field of microorganisms, and relates to trichoderma longibrachiatum A-1 and application thereof. The Trichoderma longibrachiatum A-1 provided by the invention has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.41510, the preservation number of the Trichoderma longibrachiatum A-1 is CGMCC No.41510, and the Trichoderma longibrachiatum A-1 is a Trichoderma longibrachiatum A-1. The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The trichoderma longibrachiatum A-1 has an antagonistic effect on multiple pathogenic bacteria and inhibits reproduction of the multiple pathogenic bacteria, sterile fermentation liquor of the trichoderma longibrachiatum A-1 has a remarkable inhibiting effect on mycelial growth, sporulation quantity, spore germination and bud tube elongation of fusarium oxysporum, and the strain or a fermentation product of the trichoderma longibrachiatum A-1 has remarkable advantages in the aspect of preventing and treating plant root rot.

The invention provides a botanical fungicide of a chaulmoogra seed extract, which takes sub-chaulmoogra seed oleic acid as a marker, and application of the botanical fungicide in preparation of medicines for preventing and treating plant diseases caused by pathogenic fungi and oomycetes. The hydnocarpus anthelmintica extract is obtained by extracting hydnocarpus anthelmintica seeds with a plurality of different types of organic solvents such as ethanol, petroleum ether and the like and treating the seeds by a physical method, and the hydnocarpus anthelmintica extract contains sub-hydnocarpus anthelmintica oleic acid as markers in different proportions. The extract has the effects of inhibiting conidium germination, bud tube growth or appressorium formation of fungi and oomycetes, effectively preventing pathogenic bacteria from infecting host plants and preventing and treating important plant diseases such as rice blast, mango anthracnose, potato late blight and rice false smut.

The present invention pertains to use of a composition comprising propionic acid and / or salt thereof and at least one fatty acid having 6 to 12 carbon atoms for reducing dormant spore viability, for inactivating dormant conidia, for eliminating or reducing germination of conidia, for increasing cell damage and / or cell death of hyphae, and / or for reducing or eliminating germ tube formation.

The application discloses use of an extract of black tiger in preparation of a fungicide for preventing and treating plant diseases caused by pathogenic fungi and oomycetes. The black tiger extract has the effects of inhibiting spore germination, germ tube growth or zoospore formation, and vegetative hypha growth of the fungi and oomycetes, can effectively prevent growth of the pathogenic fungi and infection on host plants, and has certain prevention and treatment effects on rice rice blast, rice rice smut, potato late blight, cucumber anthracnose, grape anthracnose, corn ear rot, pepper blight and camphor blight, and provides a possibility for development of a plant source fungicide with diversified effective components, high prevention and treatment efficiency and a wide spectrum of prevention and treatment objects.

The present application discloses a strain of antagonistic bacteria MK94‑B2 against red blight of Masson pine and its application, and relates to the field of microbial technology. The present application isolates and obtains antagonistic bacteria MK94‑B2 against red blight of Masson pine from branches of Masson pine, and the deposit number is CCTCC NO: M 20241586. Experiments have confirmed that the strain has a significant inhibitory effect on the conidia germination and germ tube elongation of the red blight pathogen of Masson pine. When treated with MK94‑B2 fermentation filtrate diluted 2.5 times, the spore germination rate of the pathogen of red blight of Masson pine is 0%, and the inhibitory effect is significant, and it can be used to prevent and treat red blight of Masson pine. In addition, the strain has an inhibitory effect on 8 important forest pathogenic fungi, especially on teak brown spot pathogen Alternaria alternata, Masson pine red blight pathogen Lushan pseudodisc multi-hairy spore and poplar leaf multi-septate sclerotinia, and can be used as a broad-spectrum antibacterial agent.

The application discloses a kind of egg worm grass special environment climate incubator and process method thereof, belong to the technical field of fungi cultivation, including the following steps: the bird egg inoculated with cordyceps strain is placed in incubator, the temperature in the box is controlled to be the temperature of colonization, humidity is the humidity of colonization, close illumination and send air with the wind speed of colonization, so that cordyceps spore is oriented to germinate inside bird egg;Real-time monitoring of carbon dioxide concentration in the box, dynamically adjust temperature, humidity and ventilation frequency according to the comparison result of monitoring value and multiple preset concentration threshold, so that mycelium proliferates in bird egg;By constructing directional airflow coupling carbon dioxide dynamic feedback control mechanism, the eggshell micro-domain gas environment is fundamentally optimized, low-speed directional air supply forms stable water vapor pressure difference and oxygen concentration gradient on both sides of eggshell stoma, guiding spore germ tube to invade unidirectionally along stoma channel.

This invention discloses a method and system for constructing crop disease models based on the coupling of multiple environmental factors, belonging to the field of smart agriculture and plant protection technology. The method includes: acquiring environmental data, soil data, crop data, and pathogen data; calculating the spore attachment success rate based on the pathogen data; dividing the spore infection process into four stages: dormancy, germination, germ tube, and infection spike stage, constructing an infection state machine model to maintain the spore stage state and calculate germination progress and cumulative infection values, thereby calculating the infection activity output; calculating water stress development correction coefficients, nutrient stress development correction coefficients, pesticide application pathogen mortality correction coefficients, and carbon dioxide content stress correction coefficients based on soil and crop data; and multiplying the spore attachment success rate, infection activity output, and multiple correction coefficients to obtain the disease risk value. This invention enables the reuse of a single model framework for multiple diseases, significantly reducing the development cycle and cost of new disease models.

The present invention discloses CaKnot1-U5 protein and its application. The present invention discovered for the first time that the pepper phytophthora effector NLP779 induces pepper to produce antimicrobial peptides. The domain enrichment analysis of the up-regulated proteins in the TMT omics results was performed, and it was found that a class of Knot1 antimicrobial peptides were all up-regulated. When the exogenously purified Knot1 (i.e., CaKnot1-U5 protein) was added to the zoospores of pepper phytophthora, it was possible to inhibit zoospore activity, germ tube elongation, and appressorium formation. Spraying the pepper phytophthora effector NLP779 on pepper plants can induce an immune response in peppers and inhibit the infection of pepper phytophthora. The combination of NLP779 and CaKnot1-U5 can better prevent and control pepper phytophthora.

This disclosure relates to a method for preparing a compound represented by formula (I), comprising reacting a compound represented by formula (II) with compounds represented by formula (III) and formula (IV) under the catalytic presence of a catalyst and the presence of a fluoride salt to obtain the compound represented by formula (I). The compound represented by formula (I) prepared by this method exhibits antifungal activity and can be used as a fungicide. Its pesticide composition can be used to control plant diseases. The compound can effectively prevent pathogens from infecting plants by inhibiting conidial germination, germ tube growth, or appressorium formation. It can be used to control devastating plant diseases, including rice blast, anthracnose, downy mildew, phytophthora, and powdery mildew, providing a new option for plant protection. #imgabs0#

The application discloses a fusion polypeptide CT01 with the functions of inducing plant hypersensitive response and inhibiting bacteria. The amino acid sequence of the fusion polypeptide CT01 is shown as SEQ ID NO. 1, and the nucleotide sequence of the coding gene is shown as SEQ ID NO. 2. After injecting the fusion polypeptide CT01 with a concentration of 15 μg / mL into leaves of Nicotiana benthamiana, obvious cell death can be observed; treating Magnaporthe grisea conidia on a hydrophobic glass slide with the fusion polypeptide CT01 with a concentration of 30 μg / mL can significantly inhibit the germ tube emergence of the Magnaporthe grisea conidia; adding the fusion polypeptide CT01 into a Magnaporthe grisea conidia suspension to a final concentration of 30 μg / mL and spraying rice seedlings can significantly inhibit the occurrence of rice blast. Therefore, the fusion polypeptide CT01 can play a role in preventing and controlling rice blast from the aspects of inducing rice disease resistance and inhibiting Magnaporthe grisea infection.