A kind of biophysical method and application of inducing Agarwood to form fragrance

A biophysical and soil agarwood technology, applied in the field of biological agriculture, can solve the problems of low yield of agarwood, uneven quality, long period of incense formation, etc., and achieve the effects of short breeding cycle, low cost and strong practicability.

Active Publication Date: 2021-08-31
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, artificially induced aroma technology mainly includes physical methods, such as "cutting method", "drilling method", "fire hole" and "opening incense door", biological methods, such as "inoculation method", and chemical methods , such as the "chemical reagent instillation method", etc., but these single methods generally have a longer cycle of agarwood, a lower yield of agarwood, and uneven quality
At present, there is still a lack of an effective method that combines physical methods and biological methods to improve the yield and quality of incense.

Method used

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  • A kind of biophysical method and application of inducing Agarwood to form fragrance
  • A kind of biophysical method and application of inducing Agarwood to form fragrance
  • A kind of biophysical method and application of inducing Agarwood to form fragrance

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Experimental program
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Effect test

Embodiment 1

[0054] Longan scorching rot fungus D2-2, Fusarium solani P13-1 and Trichoderma nigerioderma 3-4 used in the present invention have been described in the literature "Song Xiaochen et al. Combined inorganic salts, hormones and fungi to induce agarwood anti-stress Ability research, "Plant Research", 2019, Vol.39(4): 505-513".

[0055] 1. Preparation of Agarwood

[0056] (1) Inoculate PDA solid culture medium with scorch rot fungus D2-2 and Fusarium solani P13-1 respectively, and culture at 28° C. for 4 to 5 days.

[0057] (2) Choose a sunny day in July, choose an 8-year-old Agarwood tree with a diameter at breast height of 10.2cm, and chisel four 2.5cm long, 2.5cm wide, and deep 2cm grooves, and then do the same operation at intervals of 30cm along the trunk length, a total of 24 grooves are chiseled. Insert a completely red-hot iron block into the groove, stay for 30s, repeat 3 to 4 times, completely burn the xylem of Aquilaria sinensis until black, the preparation effect of t...

Embodiment 2

[0069] 1. Preparation of Agarwood

[0070] (1) Inoculate PDA solid culture medium with scorch rot fungus D2-2 and Fusarium solani P13-1 respectively, and culture at 28° C. for 4 to 5 days.

[0071] (2) Choose a sunny day in August, and choose a 10-year-old Agarwood tree with a DBH of 12.7 cm. At 50cm from the ground, at the same height of the trunk, and at a horizontal interval of 5.0cm, use a chisel to chisel out five 3.0cm long, 2.5cm wide, and 3.0cm deep, and then do the same operation at intervals of 30cm along the trunk lengthwise, and chisel out 30 concave holes in total. groove. Insert a completely red-hot iron block into the groove, stay for 40 seconds, repeat 4 to 5 times, completely burn the xylem of Aquilaria sinensis until black. After the groove is lowered to normal temperature, the PDA solid medium cultured longan scorch rot fungus D2-2 and Fusarium solani P13-1 mycelium together with the substrate (the mycelium is covered in the substrate), according to the vo...

Embodiment 3

[0083] 1. Preparation of Agarwood

[0084] (1) Inoculate PDA solid culture medium with scorch rot fungus D2-2 and Fusarium solani P13-1 respectively, and culture at 28° C. for 4 to 5 days. Inoculate Trichoderma nigerovides horse 3-4 into PDB liquid medium, and culture at 28° C. and 120 rpm for 7 days.

[0085] (2) Choose a sunny day in September, and choose a 10-year-old Agarwood tree with a DBH of 11.1 cm. At a distance of 50cm from the ground and at the same height, use a chisel to chisel out five grooves with a length of 2.5cm, a width of 2.0cm, and a depth of 2.5cm at an equal interval of 4.5cm, and then do the same operation every 30cm along the radial direction of the trunk. A total of 30 grooves were chiseled. Insert a completely red-hot iron block into the groove for 40 seconds, repeat 3-4 times, completely burn the woody part of the agarwood to black. After the groove was down to normal temperature, the longan scorch rot fungus D2-2 and the Fusarium solani P13-1 my...

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PUM

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Abstract

The invention discloses a biophysical method for inducing agarwood to form an aroma and an application thereof. The method disclosed by the invention comprises three steps of strain preparation, groove preparation and strain inoculation. The invention utilizes the combination of burn wound surface and fungal induction to promote the activation of hyphae, increase the infection rate, and shorten the induction time of incense. The method for inducing incense formation of the present invention is low in cost, easy to operate, and strong in practicability, and is suitable for a large number of agarwood tree growers to carry out artificial agarwood formation. The content of linalool extract conforms to the national pharmacopoeia standard.

Description

technical field [0001] The invention belongs to the technical field of bio-agriculture, and in particular relates to a biophysical method for inducing agarwood to form an aroma and an application thereof. Background technique [0002] Aquilaria sinensis (Lour.) Gilg, also known as Baimuxiang, is an evergreen tree of the genus Aquilaria (Aquilaria spp.) in the family Thymelaceaceae. It is naturally distributed in Hainan, Guangdong, Guangxi, Fujian, Taiwan and other provinces. Agarwood is a national second-class key protected plant. Agarwood is a secondary metabolite produced when agarwood plants are stimulated or damaged, and is a traditional precious medicinal material. Traditional Chinese medicine believes that agarwood is mild in nature, pungent and bitter in taste, and has the effects of lowering qi, regulating the middle, warming the kidneys, relieving pain, and relaxing muscles. Modern studies believe that agarwood has the functions of lowering blood pressure, sedatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/30A01N63/38A01P21/00
CPCA01N63/30
Inventor 周再知宋晓琛宋杰黄桂华王西洋梁坤南杨光张青青
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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