69results about How to "Infection effect is good" patented technology

A vegetative additive for the mixed feed used to culture quatic animals is proportionally prepared from adhesive, allicin, and 8 Chinese-medicinal materials including dandelion herb, phellodendron bark, scutellaria root, liquorice root, etc. Its advantage is high effect to kill bacteria and virus and promote the development of immune organs.

The invention provides a method for rapidly identifying resistance to verticillium wilt of eggplant. The method comprises the following steps of: accelerating germination and sowing of eggplant seeds, seedling culturing, preparation of a verticillium wilt spore suspension, treatment of pathogenic bacteria infection and incidence investigation. According to the invention, eggplant seedlings are infected with verticillium wilt in a manner of soaking and infecting seedling roots by a bacteria solution, and the eggplant seedlings are infected after being uniformly cultured in a substrate until achieving a state of one seedling with two leaves, so that the growth vigor and size of the eggplant seedlings are easy to control, and the consistency and reliability of an identification result are ensured. By adopting the appropriate verticillium wilt substrate and adjusting the concentration of the verticillium wilt suspension, the method has the advantages of good infection effect, high survival rate of the infected seedlings, uniform inoculation and rapid incidence. According to the invention, only different plants with consistent growth vigor need to be soaked in the bacteria solution after root cutting, so that the operation is simple and convenient, the seedling culture time is short, and the method is particularly suitable for identification of resistance to verticillium wilt of eggplant breeding materials in batches.

The invention belongs to the field of detection of disease resistance of horticultural plants, and relates to a seedling stage identification method for cowpea wilt resistance. The method includes the steps of sprouting cowpea seeds, sowing, cultivating seedlings, separating and cultivating bacterial strains special for inoculation, preparing oxysporum spore suspension liquid, infecting pathogenic bacteria, surveying plant disease causes and the like. Cowpea seedlings are infected with oxysporum artificially with the method that seedling roots are infected with bacterium liquid, cowpea seeds are cultivated in seedling cultivating matrixes to grow two leaves and one bud, then the seedlings are pulled out from infection, the growth vigour and the size of the cowpea seedlings can be controlled easily, the cowpea seedlings can be pulled out easily, and consistency and reliability of identification results are guaranteed. The special indication bacterial strains, the appropriate infection time and the appropriate oxysporum spore suspension liquid concentration are adopted, the infection effect is good, and even inoculation and quick disease generation are achieved. By means of the method, the oxysporum infection only needs to be conducted on different plants with the consistent growth vigour after roots are damaged directly through natural friction, the method is easy and convenient to operate, quick in identification, reliable in result and particularly suitable for identifying the oxysporum disease resistance of cowpea cultivating materials in batches.

The invention discloses a green feed additive and a preparation method thereof. The green feed additive is prepared by mixing the following components in portion by weight: 40 to 60 portions of cortex eucommiae extract, 10 to 20 portions of streptococcus faecalis powder, 30 to 40 portions of polysaccharide peptide powder and 8 to 20 portions of folium cortex eucommiae finemeal, wherein the cortex eucommiae extract at least contains 0.2 percent of chlorogenic acid; the polysaccharide peptide powder is prepared by concentrating, decoloring, precipitating with alcohol and drying the ferment liquid which is obtained by fermenting the streptococcus faecalis powder. The components of the invention are integrally applied, which can remarkably improve the function of an animal immune system, strengthen disease preventing ability of organism, and resist the intrusion of pathogen; the green feed additive contains virus resisting the immunodeficiency which can bidirectionally adjust the function of cellular immunity and greatly improve the survival rate of feeding animals; and under the conditions without hormone, the green feed additive can promote the growth and breed of animals, greatly improve the quality and taste of eggs and meat, and keep the meat quality more fresh and more close to wild taste.

The invention relates to a preparation method and applications of an H5N1 subtype bird flu pseudovirion. Specifically, the application relates to a bird flu pseudovirion with packaging plasmid and the HA gene and NA gene of the highly virulent form of the bird flu virus and a preparation method and applications thereof; and the application also relates to a system for preparing the pseudovirion.

The invention discloses application of polypeptide in preparing a medicine for treating enterovirus infection, belongs to the field of infectious disease prevention and treatment, further provides a medicinal composition for treating enterovirus infection, and in addition, provides a polypeptide preparation prepared from pharmaceutically acceptable auxiliary materials or auxiliary components. The medicinal composition comprises an effective dosage of the polypeptide as an active component. The polypeptide disclosed by the invention can be used for treating enterovirus infection, particularly for treating and preventing hand-foot-mouth diseases and herpetic herpangina, and is remarkable in effect and good in security.

The invention relates to a recombinant adeno-associated virus vector. The recombinant adeno-associated virus vector comprises constitutively active human Nrf2 (Nrf2 (CA)), a CMV promoter / enhancer, beta-globin intron for increasing gene expression, human growth hormone poly (A) tail sequence, etc. The invention also relates to a preparation method of the recombinant adeno-associated virus vector and the application of the recombinant adeno-associated virus vector or a composition containing the recombinant adeno-associated virus vector in preparing a medicine for treating glaucoma retinal ganglion cell denaturation. The recombinant adeno-associated virus vector provided by the invention can obviously reduce the intraocular pressure of a mouse, increase the survival rate of RGCs of the mouse, and effectively treat glaucoma retinal ganglion cell pathological changes.

Production of various Aeromonas hydrophila protective antigen monovalent and multivalent vitelline antibody and its usage in prevention of aquatic animal diseases are disclosed. It utilizes minor-protective antibody to make immune irritant composite egged hen and produce vitelline antibody product. It's safe, efficient, fast, has no harm and residue. It has more output, better immune-system development and functions of nutrients-added, disease-prevention and health-care.

The invention discloses Ab Ata protein immunogenicity and provides a protein which comprises Ab Ata protein N-terminal alpha-spiral part 39 amino acids and an adjuvant protein; the amino acid sequences of the Ab Ata protein N-terminal alpha-spiral part 39 amino acids are 127th-165th sites of the sequence 2 respectively. According to the Ab Ata, the N-terminal alpha-spiral part 39 amino acids are cut out, fused with CTB and expressed in BL21. Through ni-sepharose purification, peritoneal immunity is performed at 40 mu g / mice, the immunogenicity and the immune protective of the Ab Ata protein are verified through animal experiments, and the Ab Ata protein has excellent Ab infection resistance.

The invention discloses Daldinia childiae L2Ma and application thereof in preparation of bacterium grain wood, belonging to the technical field of microorganisms. The strain is collected in China General Microbiological Culture Collection Center; the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing City; and the collection number is CGMCC No:5954. The strain has excellent impregnation capability for a wood-based material, the impregnation time is short, and the impregnation depth is large; beautiful colors and grains can be formed on the surface of the material; and the physical mechanical strength is not influenced. A method for preparing a bacterium grain wood by using the strain comprises the steps of strain activation, expanding propagation and inoculation culture; and the method comprises the following specific steps: inoculating the strain on an activation and expanding propagation culture medium, performing activation and expanding propagation at 22-30 DEG C for 7-15 days, sterilizing the wood-based material, enabling the wood-based material to be sufficiently in contact with the strain subjected to activation and expanding propagation, and culturing at 22-30 DEG C for 6-8 weeks. According to the invention, the method is reasonable in process and simple to operate; and the prepared bacterium grain wood has favorable decoration effect and has no influence on the physical mechanical property of the base material, thereby being beneficial to large-scale production and popularization.

The invention provides a fermentation composition assisting in preventing COVID-19. The composition is suitable for vast social masses, has good population acceptability, can improve the immunity andanti-infection ability of the masses, and has a good effect of auxiliary prevention of COVID-19 infection. The traditional Chinese medicine composition disclosed by the invention has a synergistic effect. By increasing RAW267.4 cell proliferation and inhibiting release of IL-2, IL-6 and TNF-alpha, the immunity of an organism is improved; and by improving the immunity, the effect of resisting COVID-19 infection is achieved, and the composition is an ideal fermentation composition for assisting in preventing new coronavirus infection.

A health-care Chinese-medicinal food in the form of soft capsule for improving the immunity to infection of digestive tract and treating the inflammation in digestive tract is prepared from 5 Chinese-medicinal materials including portulaca, houttuynia, garlic clove, ginger, etc.

The invention discloses mycorrhizal inoculum and a preparing method and use thereof, wherein the preparing method of the mycorrhizal inoculum comprises: adding maitake bacteria liquid to a specific solid medium with a proportion of 37.5-75 ml per100g, and cultivating three weeks at a temperature of 25 DEG C so as to obtain the mycorrhizal inoculum, wherein the solid medium is formed by evenly mixing turf, vermiculite and perlite with a weight ratio of (7.5-6.5): (1.25-1.75): (1.25-1.75). The preparing method of the mycorrhizal inoculum is low in cost, high in efficiency, simple and easy to carry out, and is suitable for factory mass production; and the mycorrhizal inoculum prepared and obtained through the method has a good infection effect on Chinese chestnut plants and can be used for effectively improving drought-resistant and disease-and-pest-resistant capacity of the Chinese chestnut plants.

The invention discloses a traditional Chinese medicine composition and gauze mask for preventing corona virus disease 2019, and belongs to the field of traditional Chinese medicine and pharmacy. The traditional Chinese medicine composition is prepared from the following traditional Chinese medicines of 1-100 parts of radix astragali, 1-100 parts of bighead atractylodes rhizomes, 1-100 parts of divaricate saposhnikovia roots, 1-100 parts of honeysuckle flowers, 1-100 parts of fructus forsythiae, 1-100 parts of reed rhizomes, 0.9-90 parts of herba ephedrae, 0.6-60 parts of radix glycyrrhizae preparata, 2-200 parts of radix bupleuri, 1-100 parts of radix scutellariae, 1-100 parts of processed pinellia tubers and 1.5-150 parts of radix codonopsis. The traditional Chinese medicine composition is made into nanometer powder. A preparation method comprises the steps of mixing and merging the medicinal materials, and performing crushing through a low-temperature superfine crushing machine at the crushing temperature of minus 196 DEG C to obtain granules of which the granule diameters are 1-100nm. The traditional Chinese medicine composition nanometer powder is stuffed to medicine carrying tablets, and the medicine carrying tablets are applied to the gauze mask. The gauze mask has the function of resisting the corona virus disease 2019, and is good in the effect of preventing the coronavirus disease 2019.

The present invention provides a method of tertiary purification for extraction and purification of viral particles from a sample containing host cells that package viral particles. The third-stage purification method comprises: using a first fractionation solution to precipitate and remove most of the impurity protein; using a second fractionation solution to precipitate and collect virus particles and to remove some residual impurities; and further using column chromatography on the collected virus particles to remove remaining impurities, thereby obtaining an ultrapure virus solution. The method also includes the steps of collection of viral host cells, cell suspension, and cell lysis. The purification method is simple and convenient to operate, and it is convenient to obtain a high-yield ultra-purity virus solution, which is suitable for large-scale and small-scale preparation, and the solution and the operation steps do not contain toxic substances to the cells. Therefore, the method demonstrates a highly desirable purification technique for vector viruses including adeno-associated viruses and adenoviruses. The invention also includes kits or packages designed in accordance with the above techniques.

The invention provides a micro-ecology compound traditional Chinese medicine preparation and a preparing method and application thereof. The micro-ecology compound traditional Chinese medicine preparation comprises a micro-ecology compound traditional Chinese medicine composition and is characterized in that the composition comprises probiotics and compound traditional Chinese medicine; the probiotics are Lactobacillus acidophilus and / or fermentation products thereof; the compound traditional Chinese medicine comprises rhizoma coptidis, folium mori, taraxacum mongolicum and fructus forsythia.The micro-ecology compound traditional Chinese medicine composition and the preparation thereof are good in antibacterial activity and capable of well preventing and treating sow reproductive tract infection.

The present invention discloses a strain of Xylaria venosula J22QIU and an application of the Xylaria venosula J22QIU in preparation of fungi pattern wood, and belongs to the technical field of microorganisms. According to the present invention, the strain is preserved in the China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, 3# Court No.1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No:5964; the strain provides excellent dip dyeing ability for the wood base material, the dip dyeing time is short, the dip dyeing depth is high, beautiful color and texture can be formed on the material surface, and the physical and mechanical strength is not affected; the method for preparing the fungi pattern wood by using the strain comprises strain activation and propagation, and fungi inoculation culture, wherein the strain is inoculated on an activation and propagation culture medium, activation and propagation is performed for 10-15 days at a temperature of 22-30 DEG C, the wood base material is sterilized and completely contact the obtained strain, and culture is performed for 4-8 weeks at a temperature of 22-30 DEG C; and the method has characteristics of reasonable process and simple operation, the prepared fungi pattern wood has a good decorative effect, the physical and mechanical property of the substrate is not affected, and the method is suitable for large-scale production and promotion application.

The invention relates to virions in the field of biology and use thereof, and particularly discloses an adeno-associated virus mutant with heart targeting and use thereof. A heterologous peptide is (a) a protein composed of an amino acid sequence as shown in SEQ ID No: 15 or (b) a protein which is derived from (a), has heterologous peptide activity and is obtained by substituting, deleting or adding one or more amino acids to the amino acid sequence in (a). The recombinant adeno-associated virus constructed by using an AAV capsid protein mutant has high virion specificity, good safety, low immunogenicity, a wide use range, good infectivity to the heart, a good targeting property and long time of expressing exogenous genes in a mouse body.

The invention discloses a genetic transformation method of Iris germanica mediated by Agrobacterium tumefaciens, belonging to the technical field of plant genetic transformation. The transgenic plantsare obtained by Agrobacterium tumefaciens infection, screening of resistant calli, redifferentiation and PCR detection of resistant plants, using stem tip calli of Iris germanica as transgenic recipients. The method provided by the invention has the advantages of simple operation and high genetic transformation rate, and has great theoretical significance and application and popularization valuefor genetic transformation of Iris germanica and other Iris plants, gene function research and creation of new germplasm.

The invention discloses a method for infecting a peony seedling against a TRV vector-based virus-induced gene silencing system. TRV1 vectors and vectors of TRV-PDS, TRV-GFP and the like constructed onthe basis of TRV2 vectors are used for conducting vacuum infection on the peony seedling, symptoms of bleached peony leave, fluorescent leaves and root tissues, and the like are developed, gene silencing of a peony plant is achieved, and a simple fast low-cost method is provided for studying functions of various tissue related genes in a peony growth and development process.

The invention provides a traditional Chinese medicinal perineal care bag for perineal care. The traditional Chinese medicinal perineal care bag is prepared by soaking sterile large cotton swabs serving as a base material into a traditional Chinese medicinal liquid, wherein the traditional Chinese medicinal liquid is prepared from the following raw materials: radix achyranthis bidentatae, semen coicis, rhizoma alismatis, white peony root, rhizoma atractylodis, liquorice, rhizoma smilacis glabrae and dandelion. The traditional Chinese medicine composition provided by the invention is mainly prepared from the medicines capable of inducing diuresis for treating stranguria, clearing damp, promoting diuresis, resisting bacteria and diminishing inflammation, wherein the radix achyranthis bidentatae and the dandelion are the medicines for inducing diuresis for treating stranguria; the semen coicis, the rhizoma alismatis and the rhizoma atractylodis are the medicines for clearing damp and promoting diuresis; and the white peony root, the liquorice and the rhizoma smilacis glabrae have the effects of resisting bacteria and diminishing inflammation. The prepared one-off perineal care bag is convenient to use, and has the efficacies of inducing diuresis for treating stranguria, resisting bacteria and diminishing inflammation, and is used for preventing damp and heat morbid leukorrhoea or urinary tract infection of inpatients.

The invention relates to a medicine compound for curing and preventing the inflammatory bowel diseases suffered by the poultry, and the method for preparing such medicine compound. The ingredients and the weight percentages of the ingredients are respectively 1 to 10 percent enrofloxacin, 1 to 10 percent lincomycin hydrochloride, 1 to 10 percent latent solvent, and 100 percent other additive accessories. The preparation method is mixing and fully agitating the 1 to 10 percent enrofloxacin, the 1 to 10 percent lincomycin hydrochloride, the 1 to 10 percent latent solvent, and the other additive accessories, which are crushed and screened, according to the weight percentages, so a medicine compound for curing and preventing the inflammatory bowel diseases suffered by the poultry comes into being. The formulation of the invention is reasonable and scientific, the use is convenient, and the function of double sterilization is achieved. The enrofloxacin can effectively restrict the compounding and duplication of DNA so as to kill the bacteria, through the action onto the A subunit of the DNA gyrase. The lincomycin hydrochloride can kill the bacteria through restricting the compounding to the protein of the cells of the bacteria. Both functions together make the sterilization quick and highly efficient, and the medicine compound applicable for large-scale production and popularization.

The invention discloses a crop pesticide. The crop pesticide comprises the following components in parts by weight: 3-10 parts of antibacterial amine, 3-5 parts of potassium permanganate, 10-15 parts of glutaraldehyde and 70-80 parts of salicylic acid. The crop pesticide is low in production cost, good in effect for treating bacterial infection of crops, and low in environmental pollution after use, and the recurring rate of bacterial infection is low.

The invention discloses a mycorrhization technology of azalea sowing seedlings. The mycorrhization technology of the azalea sowing seedlings comprises the following steps: selecting rhododendron molle underyearling seeds to cultivate annual sowing seedlings; cultivating mycorrhizal fungi by using an MEA liquid culture medium to obtain ERM liquid fungi; digging small holes near the roots of rhododendron molle sowing seedlings, and injecting the ERM liquid fungi into the small holes by a pipette and covering with a seedling substrate; inoculating for once every other several days, continuously inoculating for several times, and observing the infection rate after three months. The mycorrhization technology of the azalea sowing seedlings has the main advantages that the infection effect is good, the mycorrhization of the azalea is realized, the growth of the azalea is promoted, and introduction and domestication of the azalea are promoted.

The invention discloses snake gall bulbus fritilariae loquat paste. The paste is prepared from, by weight, 5-8 parts of snake gall, 50-80 parts of bulbus fritilariae, 60-90 parts of loquat leaves and 5-10 parts of heartleaf houttuynia herbs. The selected raw materials of the snake gall bulbus fritilariae loquat paste are classic formula raw materials of traditional snake gall bulbus fritilariae loquat paste, the inherent curative effect of the snake gall bulbus fritilariae loquat paste in the prior art is kept, meanwhile, the improvement ingredient heartleaf houttuynia herbs of the proper quantity are added, the unexpected technical effect is achieved for improving the traditional snake gall bulbus fritilariae loquat paste, the outstanding promotion effect on the application effect of the paste is achieved, and the paste can be assimilated by the human body better, and has a remarkable promotion effect of improving the curative effect of medicine.

The invention discloses construction and application of an anti-bacteriophage escherichia coli chassis cell, and belongs to the technical field of gene engineering and microbial engineering. Accordingto the invention, the coding gene yafC of the LysR family transcription regulation factor YafC is overexpressed in escherichia coli BL21 so that the anti-bacteriophage capability of escherichia coliis remarkably improved; further, the anti-bacteriophage escherichia coli BL21 obtained through breeding is used for constructing a gamma-aminobutyric acid (GABA) production strain BL21-pET28a (+)-YafC-Gad, it is found that no bacteriophage infection risk exists in the whole fermentation process, the yield of the recombinant strain GABA reaches up to 278.3 +-16.8 gL, and the molar conversion rate reaches 98.4 +-0.3% when the recombinant strain GABA is used for biotransformation production of gamma-aminobutyric acid.

The invention discloses a titanium alloy for stomatology, belonging to the technical field of alloys. The oral medical titanium alloy of the present invention is composed of the following substances by weight: Cu3-9%, Al5.5-6.5%, V3.5-4.5%, Pd0.1-0.2%, Zr5-10%, In1-5%, Mn0.3-1%, the balance is Ti. The invention has good antibacterial infection effect, good biocompatibility and mechanical properties, wear resistance and corrosion resistance, and can be used in the manufacture of oral medical instruments.