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Xylaria venosula fungi and application of the same in preparation of fungi pattern wood

A technology of the genus Anthracera and fungus, applied in the field of microorganisms, can solve the problems of cost increase, decay or discoloration, loss of beautiful lines, etc., and achieve the effect of eliminating dead ends and good infection effect

Inactive Publication Date: 2014-04-30
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the naturally obtained fungal wood still has the following deficiencies: 1. The raw materials are not easy to obtain, and there is a strong chance, because the natural fungal wood can only be formed in nature when there are bacteria that can form the fungal wood. , so only a small amount of fungal wood can be found in dead branches and fallen trees in a small number of forests; 2. Natural fungal wood takes a long time to form and is affected by many climate and environmental factors, such as dryness, cold, lack of oxygen, strong sunlight, Other microbial interference, etc.; 3. Natural fungal wood is formed under natural conditions, and there is often the problem of excessive infection of certain bacteria on wood-based materials, resulting in serious loss of strength of the material, which cannot be processed and used, or the permeability is significantly increased. , Excessive inhalation of chemicals will be inhaled during post-treatment such as anti-corrosion and flame-retardant, which will double the cost; 4. Natural fungal grain wood is formed under natural conditions, and the size and shape of the wood are unpredictable, and the beautiful lines may be lost during processing 5. Natural fungus wood is formed under natural conditions and is susceptible to insect pests or other fungal infections, making the wood incomplete or forming unsightly decay or discoloration

Method used

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  • Xylaria venosula fungi and application of the same in preparation of fungi pattern wood
  • Xylaria venosula fungi and application of the same in preparation of fungi pattern wood
  • Xylaria venosula fungi and application of the same in preparation of fungi pattern wood

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preparation example Construction

[0063] The preparation method may also include a post-processing step, in which the hyphae on the surface of the fungal wood obtained after inoculation culture are scraped off, washed and dried to a moisture content of 8-10%.

[0064] The composition of PDA liquid medium is calculated in g / L: potato 160~240, glucose 10~20, natural pH.

[0065] The preferred components of the PDA liquid medium in g / L are: potato 200, glucose 15, pH 6.5-7.0.

[0066] The wood-based material in step B can be any one or more of logs, solid wood blocks, planks, veneers, wood flakes, sticks, and special-shaped wood products.

[0067] The tree species of the wood-based material in step B can be any tree species.

[0068] The tree species of the wood-based material in step B is preferably selected from the species of Populus in Salicaceae, Alnus in Betaceae, Betula, Prunus in Rosaceae, Balsa in Kapokaceae or Quercus in Fagaceae.

[0069] The tree species of the wood-based material in step B is more ...

Embodiment 1

[0080] —— Xylaria ( Xylaria venosula ) Acquisition, identification and preservation of J22QIU.

[0081] (1) Xanthomonas ( Xylaria venosula ) Acquisition and identification of J22QIU.

[0082] The carbon horn fungus described in the present invention is isolated from decayed stumps, fallen logs or dead branches of Southwest alder. Samples were collected from Jindian Park, Kunming City, Yunnan Province. 100g of decayed stumps and branches of Southwest alder with black lines in the xylem were collected, together with fungal fruiting bodies growing on them, and stored at 4°C for later use.

[0083] Cut the collected samples of decayed stumps and branches, together with the fungal fruiting bodies growing on them, into small pieces of 0.3~0.5cm×0.3~0.5cm×0.3~0.5cm with a scalpel, soak them in 75% alcohol for 10~ 15s, then sterilized by soaking in 0.1% mercury liter for 5 minutes, and then washed 3 times with sterilized distilled water. Then place it in the enrichment medium in ...

Embodiment 2

[0096] ——by the carbon horn bacteria ( Xylaria venosula ) J22QIU to prepare fungal pattern wood (liquid method).

[0097] (1) Preparation of fungal grain wood.

[0098] First, the Xanthomonas strain ( Xylaria venosula ) J22QIU was inoculated on PDA liquid medium, and cultured on a dark shaker at 28°C±2°C for 10 days to form mycelium with a diameter of 1-3cm and a suspension of mycelium spores. The composition of PDA liquid medium is calculated in g / L: potato 200, glucose 18, pH 6.5~7.0.

[0099] Then, the Southwest alder log to be inoculated was sawed into square pieces of 2cm x 2cm x 2cm (18 pieces in total), and placed in a culture bottle with a bottom diameter of 6cm and a height of 11cm. Next, pour the vermiculite into the culture bottle until it covers the wood block, add a small amount of water to moisten the wood block and vermiculite, cover the bottle cap, place it in a high-pressure steam sterilizer, and sterilize at 121° C. for 30 minutes.

[0100] Clamp the wood...

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Abstract

The present invention discloses a strain of Xylaria venosula J22QIU and an application of the Xylaria venosula J22QIU in preparation of fungi pattern wood, and belongs to the technical field of microorganisms. According to the present invention, the strain is preserved in the China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, 3# Court No.1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No:5964; the strain provides excellent dip dyeing ability for the wood base material, the dip dyeing time is short, the dip dyeing depth is high, beautiful color and texture can be formed on the material surface, and the physical and mechanical strength is not affected; the method for preparing the fungi pattern wood by using the strain comprises strain activation and propagation, and fungi inoculation culture, wherein the strain is inoculated on an activation and propagation culture medium, activation and propagation is performed for 10-15 days at a temperature of 22-30 DEG C, the wood base material is sterilized and completely contact the obtained strain, and culture is performed for 4-8 weeks at a temperature of 22-30 DEG C; and the method has characteristics of reasonable process and simple operation, the prepared fungi pattern wood has a good decorative effect, the physical and mechanical property of the substrate is not affected, and the method is suitable for large-scale production and promotion application.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a carbon horn fungus strain with short infection time and large depth, beautiful color and texture, and no influence on the physical and mechanical strength of wood-based materials and its application in the preparation of fungi-patterned wood. Background technique [0002] The fungal discoloration of wood has long been regarded as a defect of wood, which reduces the value of wood. In fact, fungal discoloration, such as lines and stains, has natural and unique characteristics. When the decay is not serious A wonderful touch. If this kind of wood with natural lines or colors is used to make handicrafts, such as vases, earrings, pens, wood veneers, etc., the added value of wood can be increased. The fungus wood with natural patterns and colors will be popular in the market, especially in the decoration, decoration and handicraft markets. [0003] However, the n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14B27K5/00C12R1/645
Inventor 邱坚何海珊伍建榕罗蓓伍建玲甘昌涛
Owner SOUTHWEST FORESTRY UNIVERSITY
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