Xylaria venosula fungi and application of the same in preparation of fungi pattern wood
A technology of the genus Anthracera and fungus, applied in the field of microorganisms, can solve the problems of cost increase, decay or discoloration, loss of beautiful lines, etc., and achieve the effect of eliminating dead ends and good infection effect
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[0063] The preparation method may also include a post-processing step, in which the hyphae on the surface of the fungal wood obtained after inoculation culture are scraped off, washed and dried to a moisture content of 8-10%.
[0064] The composition of PDA liquid medium is calculated in g / L: potato 160~240, glucose 10~20, natural pH.
[0065] The preferred components of the PDA liquid medium in g / L are: potato 200, glucose 15, pH 6.5-7.0.
[0066] The wood-based material in step B can be any one or more of logs, solid wood blocks, planks, veneers, wood flakes, sticks, and special-shaped wood products.
[0067] The tree species of the wood-based material in step B can be any tree species.
[0068] The tree species of the wood-based material in step B is preferably selected from the species of Populus in Salicaceae, Alnus in Betaceae, Betula, Prunus in Rosaceae, Balsa in Kapokaceae or Quercus in Fagaceae.
[0069] The tree species of the wood-based material in step B is more ...
Embodiment 1
[0080] —— Xylaria ( Xylaria venosula ) Acquisition, identification and preservation of J22QIU.
[0081] (1) Xanthomonas ( Xylaria venosula ) Acquisition and identification of J22QIU.
[0082] The carbon horn fungus described in the present invention is isolated from decayed stumps, fallen logs or dead branches of Southwest alder. Samples were collected from Jindian Park, Kunming City, Yunnan Province. 100g of decayed stumps and branches of Southwest alder with black lines in the xylem were collected, together with fungal fruiting bodies growing on them, and stored at 4°C for later use.
[0083] Cut the collected samples of decayed stumps and branches, together with the fungal fruiting bodies growing on them, into small pieces of 0.3~0.5cm×0.3~0.5cm×0.3~0.5cm with a scalpel, soak them in 75% alcohol for 10~ 15s, then sterilized by soaking in 0.1% mercury liter for 5 minutes, and then washed 3 times with sterilized distilled water. Then place it in the enrichment medium in ...
Embodiment 2
[0096] ——by the carbon horn bacteria ( Xylaria venosula ) J22QIU to prepare fungal pattern wood (liquid method).
[0097] (1) Preparation of fungal grain wood.
[0098] First, the Xanthomonas strain ( Xylaria venosula ) J22QIU was inoculated on PDA liquid medium, and cultured on a dark shaker at 28°C±2°C for 10 days to form mycelium with a diameter of 1-3cm and a suspension of mycelium spores. The composition of PDA liquid medium is calculated in g / L: potato 200, glucose 18, pH 6.5~7.0.
[0099] Then, the Southwest alder log to be inoculated was sawed into square pieces of 2cm x 2cm x 2cm (18 pieces in total), and placed in a culture bottle with a bottom diameter of 6cm and a height of 11cm. Next, pour the vermiculite into the culture bottle until it covers the wood block, add a small amount of water to moisten the wood block and vermiculite, cover the bottle cap, place it in a high-pressure steam sterilizer, and sterilize at 121° C. for 30 minutes.
[0100] Clamp the wood...
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