35 results about "Xylaria sp." patented technology

The invention discloses application of a xylaria nigripe extract in a skin whitening and moisturizing cosmetic. The inventor finds that the xylaria nigripe extract has the better moisturizing effect, can effectively inhibit the activity of in-vitro tyrosinase and has the effect of skin whitening for the first time. According to the application of the xylaria nigripe extract in the skin whitening and moisturizing cosmetic, the application field of xylaria nigripe is broadened, and the economic using value of xylaria nigripe is improved.

The invention discloses a Xylaria nigripes fermented liquid powder, the preparation and application thereof. The Xylaria nigripes fermented liquid powder is obtained by carrying out the liquid fermentation of Xylaria nigripes, obtaining mycelium and fermented liquid through solid-liquid separation, filtering monosaccharide, amino acid and other small-molecular organic compounds and inorganic salts of the fermented liquid by an ultrafiltration membrane, then drying and grinding. The powder is characterized in that the powder excludes mycelium, eliminates a plurality of inorganic salts, monosaccharide and amino acid of the fermented liquid and preserves the main active component of the fermented liquid such as Xylaria nigripes polysaccharide. The Xylaria nigripes fermented liquid powder canbe used for preparing medicine, health care product and food for regulating immunity, improving sleep, resisting anxiety and depression, curing migraine, relieving cough, resisting inflammation and curing phthisis, HBV hepatitis, prostatitis, tumor, female dysmenorrheal and diabetes.

The invention provides a method for cultivating the fruiting body of Xanthomonas vertical stripes in a liquid. The steps are as follows: preparing a liquid medium: peeling potato slices, adding water and boiling them, filtering and taking the filtrate to cool slightly; weighing yeast powder, glucose, peptone, diphosphate Potassium hydrogen, magnesium sulfate, vitamin B1, add potato juice, stir until dissolved to obtain a liquid culture medium; bottling and sterilization; inoculation: in a sterile environment, the liquid culture medium is inserted into the liquid culture medium with the vertical stripes of Xanthomonas The solid medium of mycelium or one or two fruiting bodies; static culture in a cool place at 25-30°C, the lawn will start to grow in about ten days, and the fruiting bodies will start to grow after another week. It may also include the step of inoculating and culturing the fruiting body again with its liquid culture medium after picking the fruiting body of C. vertical stripes. The method of the invention has simple process and is easy to implement, and the hyphae can directly grow into fruiting bodies in liquid culture medium, which greatly improves the bacteria production efficiency, and is denser than the fruiting bodies cultured in solid, thus providing a guarantee for increasing yield.

The invention provides a polysaccharide producing endogenous xylaria UT-X strain and belongs to an endogenous xylariaceae sp. strain. The strain is preserved in China Center for Type Culture Collection, the address is Wuhan University Wuhan China, the postcode is 430072, the preservation date is January 17th 2019, a preservation number is CCTCC M 2019055, and a 16S rDNA sequence of the strain is shown as SEQ ID NO.1. The endogenous xylaria UT-X strain can be applied to fermentation to prepare polysaccharide. The strain disclosed by the invention can perform large-scale fermentation culture ina short time; furthermore, fermentation cost is lower, and limits of conditions of areas, seasons and the like are avoided; thus, the current situation that water shield natural resources are increasingly scarce can be solved through a microbial fermentation way, so that higher economical benefits are achieved.

The invention relates to an artificial culture method of bacteria, which discloses an artificial culture method of xylaria gracillima. A culture bottle is sterilized at the temperature of 120 DEG C and then naturally cooled down, a mixed culture medium is arranged in the culture bottle, the culture bottle is sterilized at the temperature of 120 DEG C again and then naturally cooled down, and the culture bottle is closed after xylaria gracillima strains are inoculated; after the xylaria gracillima is cultured at the temperature of 20 DEG C, a natural culture method is used for obtaining maturexylaria gracillima sporocarp which is washed by sterile water and sterilized by alcohol with the concentration of 70% and then the sporocarp is chopped into block-shaped clinohedrals in the length of3mm, the clinohedrals are inoculated on the improved culture medium, after hoar hyphae grow from the inoculation culture medium, culturing is continued for 1 to 2 days, and thus xylaria gracillima mycelia are obtained, the mycelia are slowly formed by growing development to become xylaria gracillima finally. The technique solves the problem that xylaria gracillima strains are difficult to be cultured into strains for production, and has the advantages of simple process, great mass growth, high efficiency, low cost and good social benefits and application prospect.

The invention discloses a fungi Xylaria sp.FDYS-1, biological agents prepared by the fungi Xylaria sp.FDYS-1 and application of the fungi Xylaria sp.FDYS-1 in prevention and cure of pine wood nematodes. The fungi Xylaria sp.FDYS-1 is preserved in China Center for Type Culture Collection on February 25th, 2008 and the preservation No. is CCTCC NO:M 208025. The invention inoculates the Xylaria sp.FDYS-1 into a potato glucose liquid medium or a potato sugar liquid medium; the pH of the liquid medium is 5 to 8; the culture temperature is 26 DEG C to 29 DEG C; after cultivation of 3 to 5 days under nonluminous conditions, the culture medium is centrifugally filtered, and the biological agents are obtained by collection of filtrate. The biological agents have high killing activity on the pine wood nematodes, good thermal stability, environment-friendly source without toxity, strong selectivity and small ecological environmental influence. The fungi Xylaria sp.FDYS-1 has low culture condition requirements, basically maintains stable inheritance of the capacity for generation of active materials, and has good development and application prospect.