The present invention discloses a strain of Xylaria venosula J22QIU and an application of the Xylaria venosula J22QIU in preparation of fungi pattern wood, and belongs to the technical field of microorganisms. According to the present invention, the strain is preserved in the China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, 3# Court No.1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No:5964; the strain provides excellent dip dyeing ability for the wood base material, the dip dyeing time is short, the dip dyeing depth is high, beautiful color and texture can be formed on the material surface, and the physical and mechanical strength is not affected; the method for preparing the fungi pattern wood by using the strain comprises strain activation and propagation, and fungi inoculation culture, wherein the strain is inoculated on an activation and propagation culture medium, activation and propagation is performed for 10-15 days at a temperature of 22-30 DEG C, the wood base material is sterilized and completely contact the obtained strain, and culture is performed for 4-8 weeks at a temperature of 22-30 DEG C; and the method has characteristics of reasonable process and simple operation, the prepared fungi pattern wood has a good decorative effect, the physical and mechanical property of the substrate is not affected, and the method is suitable for large-scale production and promotion application.