35 results about "Xylaria sp." patented technology

The invention discloses application of a xylaria nigripe extract in a skin whitening and moisturizing cosmetic. The inventor finds that the xylaria nigripe extract has the better moisturizing effect, can effectively inhibit the activity of in-vitro tyrosinase and has the effect of skin whitening for the first time. According to the application of the xylaria nigripe extract in the skin whitening and moisturizing cosmetic, the application field of xylaria nigripe is broadened, and the economic using value of xylaria nigripe is improved.

The invention relates to isopimarane diterpenoid compounds separated from fermentation products of Xylariasp.YM311647 and an application thereof, belonging to the technical field of microbes. The production strain YM311647 is collected in China Center for Type Culture Collection (CCTCC) on September 19, 2012, and the collection number is CCTCC No: M2012365. Three isopimarane diterpenoid compounds A, B and C are obtained by conventional fermentation, extraction and separation of the strain YM311647. Antifungal activity tests prove that the compounds have obvious antifungal activity and potential applications in preparation of new agricultural or medical antifungal medicaments.

The invention discloses three fungi, which are respectively a Microsphaerosis sp. fungus YNAS-4 with the preservation No. of CGMCC No.4453, an Xylaria sp. fungus YNAS-6 with the preservation No. of CGMCC No.4455, and a Lasiodiplodia sp. fungus YNAS-8 with the preservation No. of CGMCC No.4456. The fungi can be inoculated onto a trunk or limb of aquilaria sinensis to induce the aquilaria sinensis to produce agilawood, and the fungi have wide application prospects in the aspects of improving the yield of the traditional Chinese medicine of agilawood, protecting the natural resource of aquilariasinensis and the like.

The invention relates to a preservative film with an antibacterial function and a preparation method of the preservative film. The preservative film is prepared by the following components in parts by weight: 100 parts of polyethylene, 2 to 3 parts of polydimethylsiloxane biquaternary ammonium salt, 5 to 6 parts of konjak, 7 to 9 parts of pomegranate bark, 1 to 2 parts of onion juice, 3 to 5 parts of eleocharis tuberosa peel acid alcohol extractive, 5 to 7 parts of shaddock ped extractive, 3 to 4 parts of chitin, 1 to 2 parts of starch, 2 to 3 parts of citric acid and 4 to 5 parts of glycerinum. The invention provides the preservative film with the antibacterial function and the preparation method of the preservative film; the prepared preservative film has antibacterial and bacteriostatic functions, and is applicable to retaining freshness of daily fruits and vegetables; the antibacterial function of the preservative film is effectively improved; breeding of microorganisms is reduced; the breeding of bacillus, pseudomonas, trichoderma, saccharomycetes, basidiomycetes, xylaria, streptomyces and filamentous actinomycetes and the like can be effectively inhibited; and the preservative film has the high efficient sterilizing function.

The invention relates to a simple and rapid separation and purification culture method of antler xylaria, which belongs to the technical field of special fungal culture. The antler xylaria is less reported and belongs to the xylaria. The xylaria fungus grows on a waste nest of white ants, and since the xylaria fungus contains antibacterial active substances, antioxidant active substances, anti-virus active substances, active substances for enhancing the immunity of the human body, active substances for sedation, and other active substances, the medical value is relatively high. Due to the special living environment, the xylaria resources are rare, the xylaria is difficult to deeply research and develop at present, the fresh and strong sporocarp is difficult to encounter or collect in the field, thereby bringing certain difficulty to the separation and pure culture of the fungus. The method is characterized in that a live ant nest is buried in the indoor earth, so that a great amount of xylaria rapidly grows, and the separation and purification culture is carried out by utilizing the cultured xylaria sporocarp. The method has the characteristics of economical efficiency, simplicity, rapidness, high success rate, rapidness in propagation and the like. By adopting the method, a novel concept and a novel method can be provided for large-scale artificial culture and sustainable development of the xylaria in the future.

The invention discloses a Xylaria nigripes fermented liquid powder, the preparation and application thereof. The Xylaria nigripes fermented liquid powder is obtained by carrying out the liquid fermentation of Xylaria nigripes, obtaining mycelium and fermented liquid through solid-liquid separation, filtering monosaccharide, amino acid and other small-molecular organic compounds and inorganic salts of the fermented liquid by an ultrafiltration membrane, then drying and grinding. The powder is characterized in that the powder excludes mycelium, eliminates a plurality of inorganic salts, monosaccharide and amino acid of the fermented liquid and preserves the main active component of the fermented liquid such as Xylaria nigripes polysaccharide. The Xylaria nigripes fermented liquid powder can be used for preparing medicine, health care product and food for regulating immunity, improving sleep, resisting anxiety and depression, curing migraine, relieving cough, resisting inflammation and curing phthisis, HBV hepatitis, prostatitis, tumor, female dysmenorrheal and diabetes.

The invention discloses application of a xylaria nigripe extract in a skin whitening and moisturizing cosmetic. The inventor finds that the xylaria nigripe extract has the better moisturizing effect, can effectively inhibit the activity of in-vitro tyrosinase and has the effect of skin whitening for the first time. According to the application of the xylaria nigripe extract in the skin whitening and moisturizing cosmetic, the application field of xylaria nigripe is broadened, and the economic using value of xylaria nigripe is improved.

The present invention discloses a strain of Xylaria venosula J22QIU and an application of the Xylaria venosula J22QIU in preparation of fungi pattern wood, and belongs to the technical field of microorganisms. According to the present invention, the strain is preserved in the China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, 3# Court No.1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No:5964; the strain provides excellent dip dyeing ability for the wood base material, the dip dyeing time is short, the dip dyeing depth is high, beautiful color and texture can be formed on the material surface, and the physical and mechanical strength is not affected; the method for preparing the fungi pattern wood by using the strain comprises strain activation and propagation, and fungi inoculation culture, wherein the strain is inoculated on an activation and propagation culture medium, activation and propagation is performed for 10-15 days at a temperature of 22-30 DEG C, the wood base material is sterilized and completely contact the obtained strain, and culture is performed for 4-8 weeks at a temperature of 22-30 DEG C; and the method has characteristics of reasonable process and simple operation, the prepared fungi pattern wood has a good decorative effect, the physical and mechanical property of the substrate is not affected, and the method is suitable for large-scale production and promotion application.

The invention relates to fungi and methods of isolated culture, preservation and rejuvenation, and discloses Xylaria gracillima (CGMCC No. 1625) and methods of isolated culture, preservation and rejuvenation; the isolated culture method is as follows: the main nest of odontotermes formosanus which is formed for more than three years are taken out of the natural soil layer to be put into an aseptic container, after being fully enclosed, mature fruiting bodies of the Xylaria gracillima are separated out and then are inoculated on the improved culture medium to isolate and culture the Xylaria gracillima; the preservation and rejuvenation method is as follows: after the Xylaria gracillima preserved in a medical freezer recovers, the Xylaria gracillima are inoculated to a fresh inclined culture medium in an asepsis room, the inclined culture medium is put in a freezer of 4 DEG C to be preserved after the hypha of the Xylaria gracillima overgrows on the inclined planes. The invention leads to species increasing of Xylaria, and solves the problems of difficult isolation and extraction and difficult mass industrial production of the Xylaria gracillima.

The invention discloses a xylariaceae fungus sj18 and application thereof in prevention and treatment of bursaphelenchus xylophilus, belonging to the technical field of biology. The invention providesthe effect of the xylariaceae fungus sj18 and a secondary metabolite of the xylariaceae fungus sj18 on controlling of bursaphelenchus xylophilus. The xylariaceae fungus sj18 and the secondary metabolite of the xylariaceae fungus sj18 are derived from nature, friendly to and compatible with environment and free of pollution and residues, have an efficient inhibition effect on bursaphelenchus xylophilus, can be used as pollution-free novel biopesticides, and are used for harmless prevention and treatment of pine wilt caused by infection with bursaphelenchus xylophilus.

The invention belongs to the technical field of cosmetics. The invention in particular relates to essence capable of improving acne marks and scars and a preparation method thereof. The essence is prepared from the following components, by mass: 10 to 30 percent of composition capable of improving acne marks and scars, 1 to 3 percent of butanediol, 1 to 3 percent of 1, 2-pentanediol, 0.01 to 0.06percent of sodium benzoate, 0.01 to 0.03 percent of sorbic acid and the balance of deionized water. The preparation method of the composition capable of improving acne marks and scars comprises the following steps: taking a yeast culture medium containing a geranium wilfordii extract as a fermentation substrate, inoculating saccharomycetes, and performing fermenting to obtain a primary fermentation active matter; and mixing the obtained primary fermentation active matter with a xylaria nigripes culture medium to serve as a secondary fermentation substrate, and inoculating xylaria nigripes forfermentation to obtain the essence. The essence provided by the invention can effectively inhibit excessive proliferation of fibroblasts and prevent excessive synthesis of collagen, and has an excellent effect of repairing acne marks and scars.

The invention discloses a Xylaria nigripes fermented liquid powder, the preparation and application thereof. The Xylaria nigripes fermented liquid powder is obtained by carrying out the liquid fermentation of Xylaria nigripes, obtaining mycelium and fermented liquid through solid-liquid separation, filtering monosaccharide, amino acid and other small-molecular organic compounds and inorganic salts of the fermented liquid by an ultrafiltration membrane, then drying and grinding. The powder is characterized in that the powder excludes mycelium, eliminates a plurality of inorganic salts, monosaccharide and amino acid of the fermented liquid and preserves the main active component of the fermented liquid such as Xylaria nigripes polysaccharide. The Xylaria nigripes fermented liquid powder canbe used for preparing medicine, health care product and food for regulating immunity, improving sleep, resisting anxiety and depression, curing migraine, relieving cough, resisting inflammation and curing phthisis, HBV hepatitis, prostatitis, tumor, female dysmenorrheal and diabetes.

The invention relates to isopimarane diterpenoid compounds separated from fermentation products of Xylariasp.YM311647 and an application thereof, belonging to the technical field of microbes. The production strain YM311647 is collected in China Center for Type Culture Collection (CCTCC) on September 19, 2012, and the collection number is CCTCC No: M2012365. Three isopimarane diterpenoid compounds A, B and C are obtained by conventional fermentation, extraction and separation of the strain YM311647. Antifungal activity tests prove that the compounds have obvious antifungal activity and potential applications in preparation of new agricultural or medical antifungal medicaments.

The invention relates to xylaria and a method of rejuvenation, slant cultivation and mutation and degradation prevention therefor, discloses Xylaria nigripes bacterial and a method of rejuvenation, slant cultivation and mutation and degradation prevention therefor, Xylaria nigripes bacterial, CGMCC NO.1038. The invention overcomes the problem of difficult in rejuvenation, slant cultivation and mutation and degradation prevention in the prior Xylaria nigripes bacterial. Moreover through the slant cultivation of the bacterial, the produced Xylaria nigripes bacterial mycelium can be made into a specific biological product and be applicable to foods and drugs for human.

The Xylaria radius sj18 strain and the Pichia fermentans 15B1 strain are sequentially fermented by taking residual wastes of agriculture and animal husbandry production and processing as fermentation substrates, the obtained fermentation liquor is inactivated, and the enzyme prepared by processing and production has the effect of repelling mosquitoes. The method is low in production cost, free of pollution and residues, environmentally friendly and free of toxic and side effects on animals and plants, and has important guiding significance in processing and production of biological insect repellents.

The invention relates to a bacterial fertilizer for carya illinoensis, which is characterized in that the bacterial fertilizer for carya illinoensis is composed of a fermentation broth obtained by mixed fermentation of a xylariaceae fungus (Xylariaceae fungus) sj18 strain and a pichia fermentans (Pichia fermentans) 15B1 strain or a dry powder obtained by concentrating and drying the fermentation broth. The method comprises the following steps: spraying 10 times of diluent of fermentation liquor for 1-3 times within three months from the beginning of pollen scattering of male flowers of the carya illinoensis to the fruiting of female flowers of the carya illinoensis, with the spraying amount of 1-2 liters for each tree, so that the method has the capability of remarkably maintaining and prolonging the pollen viability of the carya illinoensis, effectively improving the flowering asynchronism and increasing the pollination rate; meanwhile, black disease spots in the carya illinoensis fruit forming process are inhibited, and the phenomena that in China, the carya illinoensis forest land yield is low, flower and fruit falling is serious, and the fruit quality is poor due to the fruit black spot phenomenon are remarkably improved.

The invention provides a method for cultivating the fruiting body of Xanthomonas vertical stripes in a liquid. The steps are as follows: preparing a liquid medium: peeling potato slices, adding water and boiling them, filtering and taking the filtrate to cool slightly; weighing yeast powder, glucose, peptone, diphosphate Potassium hydrogen, magnesium sulfate, vitamin B1, add potato juice, stir until dissolved to obtain a liquid culture medium; bottling and sterilization; inoculation: in a sterile environment, the liquid culture medium is inserted into the liquid culture medium with the vertical stripes of Xanthomonas The solid medium of mycelium or one or two fruiting bodies; static culture in a cool place at 25-30°C, the lawn will start to grow in about ten days, and the fruiting bodies will start to grow after another week. It may also include the step of inoculating and culturing the fruiting body again with its liquid culture medium after picking the fruiting body of C. vertical stripes. The method of the invention has simple process and is easy to implement, and the hyphae can directly grow into fruiting bodies in liquid culture medium, which greatly improves the bacteria production efficiency, and is denser than the fruiting bodies cultured in solid, thus providing a guarantee for increasing yield.

The invention provides a polysaccharide producing endogenous xylaria UT-X strain and belongs to an endogenous xylariaceae sp. strain. The strain is preserved in China Center for Type Culture Collection, the address is Wuhan University Wuhan China, the postcode is 430072, the preservation date is January 17th 2019, a preservation number is CCTCC M 2019055, and a 16S rDNA sequence of the strain is shown as SEQ ID NO.1. The endogenous xylaria UT-X strain can be applied to fermentation to prepare polysaccharide. The strain disclosed by the invention can perform large-scale fermentation culture ina short time; furthermore, fermentation cost is lower, and limits of conditions of areas, seasons and the like are avoided; thus, the current situation that water shield natural resources are increasingly scarce can be solved through a microbial fermentation way, so that higher economical benefits are achieved.

The invention discloses a compound with the following structural formula, and provides a method for preparing the compound by fermentation culture of a Xylaria sp. strain CGMCC No. 5410. The compound has good anti-tumor activity for a plurality of tumor cells such as human non-small cell lung cancer cells, human breast cancer cells, human pancreatic cancer cells and the like, and can be used for preparing anti-tumor drugs.

The present invention belongs to the field of edible and medicinal fungi and particularly relates to a xylaria striata strain and a culture method of sclerotium of the xylaria striata strain. The sclerotium of the xylaria striata strain is rich in important components (mannitol and adenosine) for measuring medicinal value of xylaria in pharmacopeia, and the content of the important components is higher than that of many currently reported edible and medicinal fungi.

The invention relates to the field of microorganisms, in particular to a separated Xylaria sp. strain and application thereof in preparation of natural herbicides. The preservation number of the Xylaria sp. is CCTCC NO: M2020734, and a fermentation product of the Xylaria sp. can inhibit seed germination. Wherein the effective component in the fermentation product is 19,20-epoxy-cyctochalasin Q, and the compound and the hydrolysate (21-deacetyl-19,20-epoxycyctochalasin Q) thereof can significantly inhibit the germination and growth of monocotyledonous plant seeds and dicotyledonous plant seeds.The Xylaria sp. NH-1801 is high in growth speed and simple in culturing conditions; the preparation method of the active compound is simple, the yield is high, and the potential development value isachieved.

The invention discloses a method for enriching and purifying cyclopeptide Xylastriamide A from xylariastriata. The method is characterized by comprising the following steps: preparing a xylariastriata loading solution; pretreating D101 resin, packing the pretreated D101 resin into a column, and adding a loading solution for dynamic adsorption; and carrying out dynamic desorption on the cyclic peptide Xylasticamide A in the xylariastriata by using the resin. By adopting the method disclosed by the invention, the purity of the component of the cyclopeptide XylastriamideA purified by the D101 resin is greatly improved, the enrichment and purification effects are obvious, the operation is simple and convenient, the separation steps are few, the dosage of an organic solvent is small, the time is short, and the enriched and purified cyclopeptide XylastriamideA has obvious anti-tumor and nematicidal activity and is applicable to related medicines.

The invention discloses a kit for detecting the in-vitro antioxidant activity of xylaria intracellular polysaccharide and a detection method thereof. The kit comprises a detection reagent and a reaction plate, and the reaction plate comprises a bottom layer, a quantitative layer and a grinding layer which are sequentially stacked from bottom to top; a plurality of grooves for fixing cuvettes are formed in the bottom layer; a plurality of quantitative cups are arranged on the quantitative layer in a penetrating manner, and a dripping hole is formed in the bottom of each quantitative cup; a grinding cavity is formed in the grinding layer, a grinding mechanism is arranged in the grinding cavity, and a plurality of filtering holes are formed in the bottom of the grinding cavity. The kit has the functions of grinding wall breaking, extraction, filtration, quantification, standing and the like, extraction of xylaria intracellular polysaccharide can be realized only by adopting the kit, in-vitro antioxidant activity detection can be performed by matching with a spectrophotometer, the whole detection process is greatly simplified, the pollution probability of a sample solution is reduced, the reliability of a detection result is improved, and an important guarantee is provided for the research on the health-care efficacy of xylaria and related medicines.

The invention belongs to the technical field of cosmetics, and particularly relates to eye repair essence and a preparation method thereof. The eye repair essence comprises the following components inpercentage by mass of 1-4% of a hydrating agent, 0.1-0.2% of a thickening agent, 1-6% of soothing oil, 0.5-2% of an emulsifying agent, 1-12% of a xylaria nigripes fermentation product extract, 0.1-0.2% of a preservative, 0.01-0.03% of a chelating agent, 0.01-0.02% of essence and the balance deionized water. The xylaria nigripes fermentation product extract prepared by a special technology is added to the essence, so that eye melanin can be effectively eliminated, and the essence has the effects of promoting eye microcirculation, protecting and reinforcing eye capillary, smoothing fine lines and moistening, is nonirritant to skin, and can be used as a product for dispelling pigment dark eye circles and vascular dark eye circles.

The invention relates to the field of biological pest control, and discloses a pine wood nematode inhibiting composition containing oligopeptide and validamycin. The oligopeptide in the composition is extracted from xylariaceae fungus sj18, and is composed of seven amino acids. The oligopeptide has the effect of inhibiting and killing pine wood nematodes, but the oligopeptide needs higher concentration when being independently used, so that the practicability is low. The validamycin alone has no nematode killing effect. When the two components are compounded for use, a remarkable synergistic effect is achieved, the use concentration is greatly reduced, a safe and efficient nematicide is obtained, the use cost and the pollution to the environment are greatly reduced, and the main action mechanism is to regulate associated flora of nematodes to kill pine wood nematodes.

The invention belongs to the technical field of cosmetics, and particularly relates to essence capable of improving acne marks and scars and a preparation method of the essence. The composition capable of improving acne marks and scars is prepared from carbomer, an acrylic acid (acrylate) C10-30 alkanol acrylate cross-linked polymer, cetostearyl alcohol, cyclohexasiloxane, caprylic / capric triglyceride, cetostearyl glucoside, glyceryl stearate, glycerol, 1.2-butanediol, phenoxyethanol, EDTA-2Na and the balance of deionized water. The preparation method of the composition capable of improving acne marks and scars comprises the following steps: taking a yeast culture medium containing a geranium wilfordii extract as a fermentation substrate, inoculating saccharomycetes, and performing fermenting to obtain a primary fermentation active matter; and mixing the obtained primary fermentation active matter with a Xylaria nigrescens culture medium to serve as a secondary fermentation substrate,and inoculating Xylaria nigrescens for fermentation to obtain the product.

The invention discloses three fungi, which are respectively a Microsphaerosis sp. fungus YNAS-4 with the preservation No. of CGMCC No.4453, an Xylaria sp. fungus YNAS-6 with the preservation No. of CGMCC No.4455, and a Lasiodiplodia sp. fungus YNAS-8 with the preservation No. of CGMCC No.4456. The fungi can be inoculated onto a trunk or limb of aquilaria sinensis to induce the aquilaria sinensis to produce agilawood, and the fungi have wide application prospects in the aspects of improving the yield of the traditional Chinese medicine of agilawood, protecting the natural resource of aquilariasinensis and the like.

The invention relates to an artificial culture method of bacteria, which discloses an artificial culture method of xylaria gracillima. A culture bottle is sterilized at the temperature of 120 DEG C and then naturally cooled down, a mixed culture medium is arranged in the culture bottle, the culture bottle is sterilized at the temperature of 120 DEG C again and then naturally cooled down, and the culture bottle is closed after xylaria gracillima strains are inoculated; after the xylaria gracillima is cultured at the temperature of 20 DEG C, a natural culture method is used for obtaining maturexylaria gracillima sporocarp which is washed by sterile water and sterilized by alcohol with the concentration of 70% and then the sporocarp is chopped into block-shaped clinohedrals in the length of3mm, the clinohedrals are inoculated on the improved culture medium, after hoar hyphae grow from the inoculation culture medium, culturing is continued for 1 to 2 days, and thus xylaria gracillima mycelia are obtained, the mycelia are slowly formed by growing development to become xylaria gracillima finally. The technique solves the problem that xylaria gracillima strains are difficult to be cultured into strains for production, and has the advantages of simple process, great mass growth, high efficiency, low cost and good social benefits and application prospect.

The invention discloses a fungi Xylaria sp.FDYS-1, biological agents prepared by the fungi Xylaria sp.FDYS-1 and application of the fungi Xylaria sp.FDYS-1 in prevention and cure of pine wood nematodes. The fungi Xylaria sp.FDYS-1 is preserved in China Center for Type Culture Collection on February 25th, 2008 and the preservation No. is CCTCC NO:M 208025. The invention inoculates the Xylaria sp.FDYS-1 into a potato glucose liquid medium or a potato sugar liquid medium; the pH of the liquid medium is 5 to 8; the culture temperature is 26 DEG C to 29 DEG C; after cultivation of 3 to 5 days under nonluminous conditions, the culture medium is centrifugally filtered, and the biological agents are obtained by collection of filtrate. The biological agents have high killing activity on the pine wood nematodes, good thermal stability, environment-friendly source without toxity, strong selectivity and small ecological environmental influence. The fungi Xylaria sp.FDYS-1 has low culture condition requirements, basically maintains stable inheritance of the capacity for generation of active materials, and has good development and application prospect.

The invention belongs to the technical field of cosmetics, and particularly relates to a composition capable of improving acne marks and scars and a preparation method thereof. The preparation methodof the composition comprises the following steps: taking a yeast culture medium containing a geranium robertianum extract as a fermentation substrate, inoculating saccharomycetes, and carrying out fermenting to obtain a primary fermentation active matter; and mixing the obtained primary fermentation active matter with a xylaria nigripes culture medium to serve as a secondary fermentation substrate, and inoculating xylaria nigripes for fermentation to obtain the composition. According to the preparation method, saccharomycetes and xylaria nigripes are sequentially used for fermenting the geranium robertianum extract; the test finds that the obtained fermentation active matter shows new performance and can effectively inhibit excessive proliferation of fibroblasts, prevent excessive synthesis of collagen and achieve the effect of repairing acne marks and scars; and tests prove that after 28 days of continuous use, the facial brightness is increased by over 28% compared with that before use; after continuous use for 56 days, 90% of acne marks on the face of a subject are eliminated; and the method has more remarkable advantages compared with single-strain fermentation.