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34 results about "Xylaria sp." patented technology

Simple and rapid separation and purification culture method of antler xylaria

The invention relates to a simple and rapid separation and purification culture method of antler xylaria, which belongs to the technical field of special fungal culture. The antler xylaria is less reported and belongs to the xylaria. The xylaria fungus grows on a waste nest of white ants, and since the xylaria fungus contains antibacterial active substances, antioxidant active substances, anti-virus active substances, active substances for enhancing the immunity of the human body, active substances for sedation, and other active substances, the medical value is relatively high. Due to the special living environment, the xylaria resources are rare, the xylaria is difficult to deeply research and develop at present, the fresh and strong sporocarp is difficult to encounter or collect in the field, thereby bringing certain difficulty to the separation and pure culture of the fungus. The method is characterized in that a live ant nest is buried in the indoor earth, so that a great amount of xylaria rapidly grows, and the separation and purification culture is carried out by utilizing the cultured xylaria sporocarp. The method has the characteristics of economical efficiency, simplicity, rapidness, high success rate, rapidness in propagation and the like. By adopting the method, a novel concept and a novel method can be provided for large-scale artificial culture and sustainable development of the xylaria in the future.
Owner:YUNNAN UNIV

Xylaria venosula fungi and application of the same in preparation of fungi pattern wood

The present invention discloses a strain of Xylaria venosula J22QIU and an application of the Xylaria venosula J22QIU in preparation of fungi pattern wood, and belongs to the technical field of microorganisms. According to the present invention, the strain is preserved in the China General Microbiological Culture Collection Center, the address is Institute of Microbiology, Chinese Academy of Sciences, 3# Court No.1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No:5964; the strain provides excellent dip dyeing ability for the wood base material, the dip dyeing time is short, the dip dyeing depth is high, beautiful color and texture can be formed on the material surface, and the physical and mechanical strength is not affected; the method for preparing the fungi pattern wood by using the strain comprises strain activation and propagation, and fungi inoculation culture, wherein the strain is inoculated on an activation and propagation culture medium, activation and propagation is performed for 10-15 days at a temperature of 22-30 DEG C, the wood base material is sterilized and completely contact the obtained strain, and culture is performed for 4-8 weeks at a temperature of 22-30 DEG C; and the method has characteristics of reasonable process and simple operation, the prepared fungi pattern wood has a good decorative effect, the physical and mechanical property of the substrate is not affected, and the method is suitable for large-scale production and promotion application.
Owner:SOUTHWEST FORESTRY UNIVERSITY

A kind of method that utilizes liquid culture medium to produce the fruiting bodies of Xanthomonas vertical stripes

The invention provides a method for cultivating the fruiting body of Xanthomonas vertical stripes in a liquid. The steps are as follows: preparing a liquid medium: peeling potato slices, adding water and boiling them, filtering and taking the filtrate to cool slightly; weighing yeast powder, glucose, peptone, diphosphate Potassium hydrogen, magnesium sulfate, vitamin B1, add potato juice, stir until dissolved to obtain a liquid culture medium; bottling and sterilization; inoculation: in a sterile environment, the liquid culture medium is inserted into the liquid culture medium with the vertical stripes of Xanthomonas The solid medium of mycelium or one or two fruiting bodies; static culture in a cool place at 25-30°C, the lawn will start to grow in about ten days, and the fruiting bodies will start to grow after another week. It may also include the step of inoculating and culturing the fruiting body again with its liquid culture medium after picking the fruiting body of C. vertical stripes. The method of the invention has simple process and is easy to implement, and the hyphae can directly grow into fruiting bodies in liquid culture medium, which greatly improves the bacteria production efficiency, and is denser than the fruiting bodies cultured in solid, thus providing a guarantee for increasing yield.
Owner:SOUTHWEAT UNIV OF SCI & TECH

Kit for detecting in-vitro antioxidant activity of xylaria intracellular polysaccharide and detection method thereof

The invention discloses a kit for detecting the in-vitro antioxidant activity of xylaria intracellular polysaccharide and a detection method thereof. The kit comprises a detection reagent and a reaction plate, and the reaction plate comprises a bottom layer, a quantitative layer and a grinding layer which are sequentially stacked from bottom to top; a plurality of grooves for fixing cuvettes are formed in the bottom layer; a plurality of quantitative cups are arranged on the quantitative layer in a penetrating manner, and a dripping hole is formed in the bottom of each quantitative cup; a grinding cavity is formed in the grinding layer, a grinding mechanism is arranged in the grinding cavity, and a plurality of filtering holes are formed in the bottom of the grinding cavity. The kit has the functions of grinding wall breaking, extraction, filtration, quantification, standing and the like, extraction of xylaria intracellular polysaccharide can be realized only by adopting the kit, in-vitro antioxidant activity detection can be performed by matching with a spectrophotometer, the whole detection process is greatly simplified, the pollution probability of a sample solution is reduced, the reliability of a detection result is improved, and an important guarantee is provided for the research on the health-care efficacy of xylaria and related medicines.
Owner:HEFEI UNIV OF TECH

Artificial culture method of xylaria gracillima

The invention relates to an artificial culture method of bacteria, which discloses an artificial culture method of xylaria gracillima. A culture bottle is sterilized at the temperature of 120 DEG C and then naturally cooled down, a mixed culture medium is arranged in the culture bottle, the culture bottle is sterilized at the temperature of 120 DEG C again and then naturally cooled down, and the culture bottle is closed after xylaria gracillima strains are inoculated; after the xylaria gracillima is cultured at the temperature of 20 DEG C, a natural culture method is used for obtaining maturexylaria gracillima sporocarp which is washed by sterile water and sterilized by alcohol with the concentration of 70% and then the sporocarp is chopped into block-shaped clinohedrals in the length of3mm, the clinohedrals are inoculated on the improved culture medium, after hoar hyphae grow from the inoculation culture medium, culturing is continued for 1 to 2 days, and thus xylaria gracillima mycelia are obtained, the mycelia are slowly formed by growing development to become xylaria gracillima finally. The technique solves the problem that xylaria gracillima strains are difficult to be cultured into strains for production, and has the advantages of simple process, great mass growth, high efficiency, low cost and good social benefits and application prospect.
Owner:浙江三禾生物工程股份有限公司 +1

Composition capable of improving acne marks and scars and preparation method thereof

The invention belongs to the technical field of cosmetics, and particularly relates to a composition capable of improving acne marks and scars and a preparation method thereof. The preparation methodof the composition comprises the following steps: taking a yeast culture medium containing a geranium robertianum extract as a fermentation substrate, inoculating saccharomycetes, and carrying out fermenting to obtain a primary fermentation active matter; and mixing the obtained primary fermentation active matter with a xylaria nigripes culture medium to serve as a secondary fermentation substrate, and inoculating xylaria nigripes for fermentation to obtain the composition. According to the preparation method, saccharomycetes and xylaria nigripes are sequentially used for fermenting the geranium robertianum extract; the test finds that the obtained fermentation active matter shows new performance and can effectively inhibit excessive proliferation of fibroblasts, prevent excessive synthesis of collagen and achieve the effect of repairing acne marks and scars; and tests prove that after 28 days of continuous use, the facial brightness is increased by over 28% compared with that before use; after continuous use for 56 days, 90% of acne marks on the face of a subject are eliminated; and the method has more remarkable advantages compared with single-strain fermentation.
Owner:广州市万千粉丝化妆品有限公司
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