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Method for rapidly identifying resistance to verticillium wilt of eggplant

An identification method and technology for Verticillium wilt are applied in the methods of using spores, seed and rhizome treatment, botanical equipment and methods, etc., and can solve the problems of speeding up the breeding process of eggplant resistance to Verticillium wilt, and achieve easy control of size, Guaranteed consistency, reliability, and high survival rates

Inactive Publication Date: 2013-10-23
SHENYANG AGRI UNIV
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Problems solved by technology

[0004] If a method for quickly identifying the resistance to Verticillium wilt in eggplant can be found, it will undoubtedly greatly speed up the breeding process for resistance to Verticillium wilt in eggplant

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  • Method for rapidly identifying resistance to verticillium wilt of eggplant
  • Method for rapidly identifying resistance to verticillium wilt of eggplant

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Embodiment Construction

[0044] The rapid identification method of eggplant Verticillium wilt resistance comprises the following steps:

[0045] A. Germination of eggplant seeds:

[0046] Different types of eggplant germplasm resources were selected as identification objects, including Liaoqi 6, Xi'an Lvqie, Liaoqie 3, Liaoqie 5, Tianjin Kuaiyuan and Tuolubamu.

[0047] Select the seeds with full grains of the eggplant germplasm resources, and soak the seeds in warm soup at a temperature of 50°C for 30 minutes to make the seeds fully absorb water, and then place the seeds in a culture dish covered with absorbent paper for constant temperature treatment. 30°C until the seeds germinate to obtain germinated seeds;

[0048] B. Sowing and seedling cultivation:

[0049] Sowing the germinated seeds on the seedling culture medium and culturing them to obtain seedlings with two leaves and one heart;

[0050] The preparation method of the seedling culture medium is as follows: mix peat, vermiculite and chick...

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Abstract

The invention provides a method for rapidly identifying resistance to verticillium wilt of eggplant. The method comprises the following steps of: accelerating germination and sowing of eggplant seeds, seedling culturing, preparation of a verticillium wilt spore suspension, treatment of pathogenic bacteria infection and incidence investigation. According to the invention, eggplant seedlings are infected with verticillium wilt in a manner of soaking and infecting seedling roots by a bacteria solution, and the eggplant seedlings are infected after being uniformly cultured in a substrate until achieving a state of one seedling with two leaves, so that the growth vigor and size of the eggplant seedlings are easy to control, and the consistency and reliability of an identification result are ensured. By adopting the appropriate verticillium wilt substrate and adjusting the concentration of the verticillium wilt suspension, the method has the advantages of good infection effect, high survival rate of the infected seedlings, uniform inoculation and rapid incidence. According to the invention, only different plants with consistent growth vigor need to be soaked in the bacteria solution after root cutting, so that the operation is simple and convenient, the seedling culture time is short, and the method is particularly suitable for identification of resistance to verticillium wilt of eggplant breeding materials in batches.

Description

technical field [0001] The invention belongs to the field of resistant plant detection, and in particular relates to a rapid identification method for eggplant Verticillium wilt resistance. Background technique [0002] Verticillium wilt of eggplant (Verticillium wilt) is a worldwide soil-borne disease, with an incidence rate of 15%-20% in general years and over 70% in severe years, resulting in a substantial reduction in eggplant production, or even failure. It is known as the "cancer of eggplant" ", known as (Zeng et al., 2009). In recent years, with the expansion of eggplant planting area, the phenomenon of continuous cropping has become increasingly serious, which has made eggplant Verticillium wilt aggravated year by year. The disease is a disease of the whole plant, and the disease spreads from bottom to top or from half to the whole plant. Typical symptoms include four types: dead type, macular type, yellow mottled type and dwarf type (Dong Jinjie, 2001; Dong Guoju a...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01C1/02A01G1/00C12N3/00
Inventor 叶雪凌刘志勇周宝利陈志霞
Owner SHENYANG AGRI UNIV
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