Cotton verticillium wilt resistant seedling stage identification method

Abstract

The invention belongs to the technical field of cotton disease resistance breeding and specifically relates to a cotton verticillium wilt resistant seedling stage identification method. The method provided by the invention comprises the following main steps of: firstly carrying out shaking culture on cotton verticillium wilt germ 6SY2-37 collected in China Center for Type Culture Collection with the collection number being CCTCC NO: M2011095 in a Czapek's liquid medium at the temperature of 25 DEG C for 5d, filtering by the use of gauze, counting by the use of a blood cell counting panel, preparing 5*106 spore/ml, pulling up seedling with a matrix when the water-planting cotton seedling grows to a stage of two leaves and one core, cutting 1cm of water root, dipping the root for 10s and inoculating, embedding wet soil into a plastic earthen bowl, inoculating pathogen, immediately watering to prevent the cotton seedling from fading, inoculating for 10 days and causing the disease. The method provided by the invention has advantages of high survival rate of the cotton seedling after inoculation, uniform inoculated pathogen, rapid morbidity, convenient operation and good stability of results. In addition, the method takes short time, requires short seedling growing time and is convenient to manage. Therefore, the method is suitable for cotton verticillium wilt resistance identification and pathogen pathogenicity differentiation determination of large-batch materials.

Description

technical field [0001] The invention belongs to the technical field of cotton disease-resistant breeding, and in particular relates to a method for identifying cotton resistance to Verticillium wilt at the seedling stage. Crop breeding for disease resistance provides early rapid identification of resistant material. Background technique [0002] Cotton Verticillium dahliae.Kleb. is a devastating soil-borne vascular disease in the world, which seriously affects the yield and quality of cotton, and can infect a variety of herbaceous crops, tree crops, flowers and woody ornamental plants, such as strawberries , tomato, mint, olive tree, pepper, canola, eggplant, potato, sunflower, chrysanthemum, rose, maple, etc. [0003] Scholars at home and abroad generally believe that breeding and utilizing disease-resistant varieties is an economical and effective way to control cotton Verticillium wilt. Verticillium wilt resistance identification in cotton is an essential link in diseas...

AI Technical Summary

Problems solved by technology

Among them, the acupuncture method has the characteristics of forced inoculation, which cannot accurately reflect the resistance of cotton seedlings; the method of tearing the bottom of the paper pot and dipping the roots is a greenhouse seedling identification method determined after years of experimental research in the 1970s and 1980s. It is fast and accurate. , easy to operate, etc., but making paper pots is labor-intensive and time-consuming, and it is easy to pollute when raising seedlings

The method of watering roots with bacteria liquid in bottomless plastic pots and the method of quantitatively injecting bacteria liquid in hexagonal plastic pots take a long time to grow seedlings, cause uneven root damage, and take a long time to identify

The four identification methods all have certain shortcomings, which is one of the reasons for the significant difference between the identification results at the seedling stage and the identification of artificial disease gardens

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