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55results about How to "Significant incidence" patented technology

Cotton verticillium wilt resistant seedling stage identification method

The invention belongs to the technical field of cotton disease resistance breeding and specifically relates to a cotton verticillium wilt resistant seedling stage identification method. The method provided by the invention comprises the following main steps of: firstly carrying out shaking culture on cotton verticillium wilt germ 6SY2-37 collected in China Center for Type Culture Collection with the collection number being CCTCC NO: M2011095 in a Czapek's liquid medium at the temperature of 25 DEG C for 5d, filtering by the use of gauze, counting by the use of a blood cell counting panel, preparing 5*106 spore/ml, pulling up seedling with a matrix when the water-planting cotton seedling grows to a stage of two leaves and one core, cutting 1cm of water root, dipping the root for 10s and inoculating, embedding wet soil into a plastic earthen bowl, inoculating pathogen, immediately watering to prevent the cotton seedling from fading, inoculating for 10 days and causing the disease. The method provided by the invention has advantages of high survival rate of the cotton seedling after inoculation, uniform inoculated pathogen, rapid morbidity, convenient operation and good stability of results. In addition, the method takes short time, requires short seedling growing time and is convenient to manage. Therefore, the method is suitable for cotton verticillium wilt resistance identification and pathogen pathogenicity differentiation determination of large-batch materials.
Owner:HUAZHONG AGRI UNIV

Sugarcane variety xanthomonas albilineans indoor resistance identification method

InactiveCN108486216AIntuitive performance resistance levelShow resistance levelMicrobiological testing/measurementBiological material analysisXanthomonas albilineansDuganella sacchari
The invention relates to a sugarcane variety xanthomonas albilineans indoor resistance identification method. The method includes: bacterial suspension culture of sugarcane xanthomonas albilineans pathogenic bacteria, seedling cultivation of sugarcane variety or strain, inoculation identification and disease investigation. The method identifies the sugarcane variety xanthomonas albilineans resistance by means of leaf cutting inoculation, the method is close to the condition of artificially inoculated xanthomonas albilineans pathogenesis, and can indicate the resistance level of sugarcane variety to xanthomonas albilineans visually. Indoor inoculation is not disturbed by external environmental factors, has the characteristics of significant sugarcane variety incidence, good reproducibility,strong operability, simple operation, and low requirements for experimental equipment, and is convenient to carry out sugarcane variety xanthomonas albilineans resistance identification. The sugarcane variety xanthomonas albilineans indoor resistance identification method provided by the invention provides technical support for sugarcane xanthomonas albilineans resistance breeding, can quickly and accurately identify and evaluate the sugarcane variety xanthomonas albilineans resistance, saves and resources, labors and material resources, and greatly shortens the breeding period of xanthomonasalbilineans resistant sugarcane varieties.
Owner:FUJIAN AGRI & FORESTRY UNIV

Efficient smut inoculation method for sugarcane seedlings

A smut inoculation method for sugarcane seedlings comprises the steps as follows: after seven true leaves of each seedling are grown out, the seedling is pulled out from an incubator, the middle-upper parts of the leaves are cut off, 2-3 old leaves at the base part are removed, a spore suspension with the ustilago scitaminea syd concentration of 1*10<7> spores/ml is adopted for soaking the middle-lower part of the pulled seedling for 10 min, and the seedling is sealed, placed in a room at the temperature of 25-28 DEG C overnight in a moisture preserving manner, transplanted in a nutrient bag next day and planted in the field after survived; and combination morbidity is researched during final selection, combination with the combination morbidity higher than 25% is eliminated, and seedlings with good and healthy characters are screened out to enter a next stage for observation. According to the invention, morbidity of the seedlings inoculated with ustilago scitaminea syd are remarkable, the reproducibility is good, the test cycle is short, the operability is high, the cost is low, and a large quantity of seedlings can be inoculated; and the method can be applied to a sugarcane variety breeding institution for new sugarcane variety breeding resistant to sugarcane smut.
Owner:SUGARCANE RES INST OF YUNNAN ACADEMY OF AGRI SCI

Inoculation method for appraising sugarcane mosaic disease resistance

The invention provides an inoculation method for identifying the disease resistance to sugarcane mosaic virus. The method mainly comprises the following steps: inoculating virus source living bodies which propagate are stored on a sugarcane plant; before the inoculation, young disease leaves of the plant are cut into fine pieces, ground into a homogenate in the presence of a phosphate buffer solution and filtered with double-layer gauze to obtain a filtrate which is the virus inoculant; in a cool evening during the sugarcane growth period, the aerial part of the sugarcane plant are rapidly cut along the soil surface with a sharp blade or scissors, a drop of virus inoculant is sucked immediately with a dropper and dropped into the underground wound surface of the sugarcane so that the virus inoculant contacts with the wound surface and viruses invades the plant; and the wound surface is uncovered after being shaded for 24 hours. With the method, sugarcane mosaic virus after being inoculated develops remarkably and uniformly, the sensitivity is high, and the result stability is good. The method has the advantages that the process is simple, rapid, standardized and practical; the manual rubbing of sugarcane leaves is obviated, and the head scuffing is prevented so that the operability is higher and the inoculation efficiency is higher; and the identification result is closer to the field naturally-infected result so that the method is suitable for rapidly carrying out the identifying and the screening of mosaic virus resistance to large-scale material.
Owner:SUGARCANE RES INST OF YUNNAN ACADEMY OF AGRI SCI
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