Inoculation method for appraising sugarcane mosaic disease resistance

Abstract

The invention provides an inoculation method for identifying the disease resistance to sugarcane mosaic virus. The method mainly comprises the following steps: inoculating virus source living bodies which propagate are stored on a sugarcane plant; before the inoculation, young disease leaves of the plant are cut into fine pieces, ground into a homogenate in the presence of a phosphate buffer solution and filtered with double-layer gauze to obtain a filtrate which is the virus inoculant; in a cool evening during the sugarcane growth period, the aerial part of the sugarcane plant are rapidly cut along the soil surface with a sharp blade or scissors, a drop of virus inoculant is sucked immediately with a dropper and dropped into the underground wound surface of the sugarcane so that the virus inoculant contacts with the wound surface and viruses invades the plant; and the wound surface is uncovered after being shaded for 24 hours. With the method, sugarcane mosaic virus after being inoculated develops remarkably and uniformly, the sensitivity is high, and the result stability is good. The method has the advantages that the process is simple, rapid, standardized and practical; the manual rubbing of sugarcane leaves is obviated, and the head scuffing is prevented so that the operability is higher and the inoculation efficiency is higher; and the identification result is closer to the field naturally-infected result so that the method is suitable for rapidly carrying out the identifying and the screening of mosaic virus resistance to large-scale material.

Description

Technical field: [0001] The invention relates to the technical field of agriculture, in particular to an inoculation method for identification of sugarcane mosaic disease resistance. Background technique: [0002] Sugarcane mosaic disease is a worldwide sugarcane disease. In recent years, it has commonly occurred in sugarcane areas such as Fujian, Guangdong, Guangxi, Sichuan and Zhejiang in my country. It has become the most common and most serious major disease in Yunnan sugarcane areas. The province's annual incidence area reaches more than 1 million mu, of which the serious incidence area reaches more than 500,000 mu, and there is a tendency to expand and spread and aggravate the damage year by year. For susceptible varieties, the incidence rate of severe fields is as high as 100%. Sugarcane infection generally results in 10-40% yield loss and reduced sugar content. Its pathogen is complex, and can be caused by 6 kinds of viruses of Potyviridae (Potyviridae) alone or in ...

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for identifying the resistance of sugarcane germplasm resources to mosaic diseases mainly include field disease nursery identification, field natural disease identification and artificial inoculation identification. The land area is large and the number of identification materials is limited; and due to the uneven distribution of natural pathogens and species in disease nurseries and fields, changes in climate and media and other environmental conditions often affect the authenticity, stability and accuracy of identification results; artificial inoculation The identification is quick and easy, the number of identified materials is not limited, and the environmental conditions can be controlled artificially, which can meet the needs of large-scale screening of antigen-derived materials. However, the existing two inoculation methods: spray inoculation and finger friction inoculation It is necessary to rub sugarcane leaves by hand to make wounds before inoculation. Because it is difficult to grasp the rubbing strength, it often leads to uneven rubbing of wounds, resulting in uneven disease after inoculation, unstable pathogenicity, and affecting identification results.

In addition, because the surface of sugarcane leaves is covered with hair groups, and the leaf margins are sharp, manual rubbing is easy to scratch hands, which also brings difficulties to specific operations, resulting in time-consuming and labor-intensive inoculation and low work efficiency.

Not suitable for rapid identification of mosaic disease resistance in large quantities of sugarcane germplasm resources

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