Efficient smut inoculation method for sugarcane seedlings

Abstract

A smut inoculation method for sugarcane seedlings comprises the steps as follows: after seven true leaves of each seedling are grown out, the seedling is pulled out from an incubator, the middle-upper parts of the leaves are cut off, 2-3 old leaves at the base part are removed, a spore suspension with the ustilago scitaminea syd concentration of 1*10<7> spores/ml is adopted for soaking the middle-lower part of the pulled seedling for 10 min, and the seedling is sealed, placed in a room at the temperature of 25-28 DEG C overnight in a moisture preserving manner, transplanted in a nutrient bag next day and planted in the field after survived; and combination morbidity is researched during final selection, combination with the combination morbidity higher than 25% is eliminated, and seedlings with good and healthy characters are screened out to enter a next stage for observation. According to the invention, morbidity of the seedlings inoculated with ustilago scitaminea syd are remarkable, the reproducibility is good, the test cycle is short, the operability is high, the cost is low, and a large quantity of seedlings can be inoculated; and the method can be applied to a sugarcane variety breeding institution for new sugarcane variety breeding resistant to sugarcane smut.

Description

Technical field: [0001] The invention relates to the field of sugarcane smut-resistant variety breeding, and relates to a method for efficiently inoculating sugarcane seedlings with sugarcane smut to achieve early selection of smut-resistant combinations and individual plants. technical background: [0002] Sugarcane smut is an airborne fungal disease caused by Ustilago scitaminea Syd. This disease has become the most important fungal disease in all countries and regions where sugarcane is grown. It has eliminated some high-yield and high-sugar plants. The dominant variety causes serious loss of cane stem yield and sucrose content of susceptible varieties, and the damage is even more serious on dryland sugarcane. It is generally recognized that the most economical and effective way to control the disease is to plant disease-resistant varieties. Major sugarcane-producing countries such as the United States, Brazil, Cuba and China all regard smut resistance as one of the main ...

AI Technical Summary

Problems solved by technology

Due to the large number of hybrid combinations, traditional identification methods require a lot of capital and labor input

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