The application discloses an immune detection method for accurately quantifying structural analogues and application thereof. The immune detection method can realize accurate, intuitive and
rapid identification and quantification of target analytes by combining wide-spectrum antibodies and specific antibodies to recognize structural analogues and by establishing a three-dimensional curved surface model through a multilayer
perception machine model, and can solve the problem of false positive results in the detection process due to the high
structural similarity between a certain target
analyte and another substance in the immune
analysis method, especially for analytes with indistinctive characteristic structures and cross substances, which are often difficult to prepare high-specificity antibodies. The immune detection method is simple to operate, does not require experimental personnel to take multiple
verification measures, and greatly reduces the
cost burden of detection. The application has great significance and broad application prospect for improving the accuracy of
rapid detection of residual substances in food.