Mycorrhizal inoculum and preparing method and use thereof

A technology for mycorrhizal inoculants and bacterial liquid, which is applied in the field of mycorrhizal inoculants and their preparation, can solve the problems of reduced infection effect and low infection rate of inoculum products, and achieves excellent characters, rapid reproduction and active bacterial liquid. high effect

Inactive Publication Date: 2013-08-07
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, domestic researches on inoculum products are mostly focused on the research and development of inoculum products of pine species, while research on inoculum products of chestnut is relatively less. Although common inoculum products can infect chestnut, the infection rate is not high. High, and the infection effect of most bacterial agent products gradually decreases with the emergence of problems such as long-term application of bacterial strain aging

Method used

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  • Mycorrhizal inoculum and preparing method and use thereof
  • Mycorrhizal inoculum and preparing method and use thereof
  • Mycorrhizal inoculum and preparing method and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] From May 2011 to September 2012, the inventor collected fungal fruiting bodies in chestnut forests with different terrains in the Qianxi area of ​​Tangshan City, Hebei Province. Among them, only the mycorrhizae formed by the roots of chestnuts were collected and connected with their hyphae The collected fruiting bodies were stored in an ice box at low temperature and brought back to the laboratory in time. After repeated manual separation and purification, a new chestnut ectomycorrhizal fungus strain was obtained. The result of the preliminary identification of the fruiting body morphology of this fungus is Grifola frondosa, and the result of molecular identification of the isolated mycelium further confirms that the fungus that has a good symbiotic relationship with chestnut is Grifola frondosa.

[0031] Further, after researching and confirming that the mycelium of Grifola frondosa still does not utilize the mycelia of Grifola frondosa to make bacterial agent and be ap...

Embodiment 2

[0047] In this example, several commercially available fungal agents (Thrimecia lanuginosa, Boletus versicolor and Boletus cerevisiae) are used as controls, and the mycorrhizal agent of the present invention prepared in Example 1 is verified according to the following steps The actual application effect:

[0048] First, inoculate various bacterial agents on the well-developed lateral roots of chestnut seedlings born in the current year, then regularly investigate the growth indicators of the seedlings and measure the net photosynthetic rate of their leaves, investigate the mycorrhizal infection rate, the biomass indicators of the seedlings, and the mineral elements of the leaves. content, and then through data comparison and analysis, in order to verify the actual application effect of the mycorrhizal agent prepared by the present invention.

[0049] specifically:

[0050] 1. Infestation Rate Evaluation

[0051] method:

[0052] One month after inoculation, the infection r...

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Abstract

The invention discloses mycorrhizal inoculum and a preparing method and use thereof, wherein the preparing method of the mycorrhizal inoculum comprises: adding maitake bacteria liquid to a specific solid medium with a proportion of 37.5-75 ml per100g, and cultivating three weeks at a temperature of 25 DEG C so as to obtain the mycorrhizal inoculum, wherein the solid medium is formed by evenly mixing turf, vermiculite and perlite with a weight ratio of (7.5-6.5): (1.25-1.75): (1.25-1.75). The preparing method of the mycorrhizal inoculum is low in cost, high in efficiency, simple and easy to carry out, and is suitable for factory mass production; and the mycorrhizal inoculum prepared and obtained through the method has a good infection effect on Chinese chestnut plants and can be used for effectively improving drought-resistant and disease-and-pest-resistant capacity of the Chinese chestnut plants.

Description

technical field [0001] The present invention relates to a mycorrhizal agent and its preparation method and use. Background technique [0002] At present, domestic researches on inoculum products are mostly focused on the research and development of inoculum products of pine species, while research on inoculum products of chestnut is relatively less. Although common inoculum products can infect chestnut, the infection rate is not high. High, and the infection effect of most bacterial agent products gradually decreases with the emergence of problems such as long-term application of bacterial strain aging. [0003] Therefore, it is of great significance to find an ectomycorrhizal fungus that can have a good symbiotic relationship with chestnut and can be cultivated in large quantities under artificial conditions, and make a fungal agent for production practice. Contents of the invention [0004] The present invention aims to solve at least one of the technical problems exist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04A01G31/00A01G1/00
Inventor 郭素娟柴迪迪李广会邹峰谢鹏吕文君熊欢秦天天赵国强付庆伟王凤春
Owner BEIJING FORESTRY UNIVERSITY
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