Micro-ecology compound traditional Chinese medicine preparation and preparing method and application thereof
A technology of traditional Chinese medicine microecology and compound preparations, which is applied in the direction of medical formulas, anti-infectives, drug combinations, etc., can solve problems such as the core competitiveness of pig farms and the impact of economic benefits, the reduction of host immune function, and the limitations of antibiotic therapy. Antibiotic residue problem, excellent synergistic effect, good therapeutic effect
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preparation example 1
[0073] The specific preparation method of the freeze-dried powder of Lactobacillus acidophilus BLCC2-0111 is as follows:
[0074] (I) Strains: Lactobacillus acidophilus BLCC2-0111 was selected;
[0075] (II) Slant culture: inoculate one ring of strains on a solid slant medium, and cultivate at 37° C. for 18 hours to obtain slant strains;
[0076] (Ⅲ) First-class seed liquid: pick a ring of slant strains, insert it into 1000mL sterilized liquid seed medium, and culture it at 37°C for 16 hours to obtain the first-class seed liquid;
[0077] (Ⅳ) Seed tank cultivation: With an inoculum size of 2% (v / v), connect the primary seed liquid in a sterilized seed tank containing 300L liquid seed medium, and culture it statically for 10 h at 37°C , to prepare the seed solution;
[0078] (Ⅴ) Fermentation tank culture: with the inoculum amount of 2% (v / v), the seed liquid obtained from the fermentation of the seed tank was connected to the sterilized liquid fermentation medium of the ferme...
Embodiment 1
[0086] Example 1 Screening of lactic acid bacteria
[0087] 1. Material method
[0088] 1.1 Strains
[0089] 1.1.1 Lactic acid bacteria
[0090] Select Enterococcus faecium BLFC-3-7, Lactobacillus plantarum LP, Lactobacillus acidophilus BLCC2-0111 as candidate strains. The above three strains of lactic acid bacteria were provided by the Bacteria Resource Collection Center of the Bioengineering Research Institute of Shandong Baolai Lilai Bioengineering Co., Ltd.
[0091] 1.1.2 Sow reproductive tract pathogenic strains
[0092] Three strains of pathogenic bacteria isolated from the reproductive tract of sows in a pig farm in Tai'an City, Shandong Province: Escherichia coli, Staphylococcus aureus, and Streptococcus pyogenes.
[0093] 1.2 Medium:
[0094] Lactic acid bacteria medium: MRS medium.
[0095] Pathogen culture medium: BHI brain heart infusion medium.
[0096] 1.3 Test method
[0097] 1.3.1 Preparation of lactic acid bacteria fermentation broth
[0098] Under a...
Embodiment 2
[0108] Example 2 Determination of antibacterial active substances in fermentation product of Lactobacillus acidophilus BLCC2-0111 and crude extraction of antibacterial substances
[0109] 2.1 Determination of antibacterial active substances in the fermentation product of Lactobacillus acidophilus BLCC2-0111
[0110] 2.1.1 Acid inhibition and exclusion test: Since lactic acid bacteria produce a large amount of lactic acid during the fermentation process, the pH value of the fermentation supernatant of lactic acid bacteria can reach about 4.0, and the inhibitory effect on the indicator bacteria may also be the lactic acid secreted by lactic acid bacteria. In order to exclude the interference of lactic acid, the pH values of lactic acid-adjusted distilled water and lactic acid-adjusted MRS medium were 3.0, 4.0, 5.0, and 6.0, respectively, as controls. Take 1 g of the lyophilized powder of Lactobacillus acidophilus BLCC2-0111 and its fermentation product (taken from Preparati...
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