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Daldinia childiae and application thereof in preparation of bacterium grain wood

A technology of charcoal horn bacteria and fungi, applied in the field of microorganisms, can solve the problems of inability to process and use, excessive inhalation, incomplete wood, etc., and achieves the advantages of large-scale production and popularization and application, reasonable technical route, and operation process. simple effect

Inactive Publication Date: 2014-01-22
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the naturally obtained fungal wood still has the following deficiencies: 1. The raw materials are not easy to obtain, and there is a strong chance, because the natural fungal wood can only be formed in nature when there are bacteria that can form the fungal wood. , so only a small amount of fungal wood can be found in dead branches and fallen trees in a small number of forests; 2. Natural fungal wood takes a long time to form and is affected by many climate and environmental factors, such as dryness, cold, lack of oxygen, strong sunlight, Other microbial interference, etc.; 3. Natural fungal wood is formed under natural conditions, and there is often the problem of excessive infection of certain bacteria on wood-based materials, resulting in serious loss of strength of the material, which cannot be processed and used, or the permeability is significantly increased. , Excessive inhalation of chemicals will be inhaled during post-treatment such as anti-corrosion and flame-retardant, which will double the cost; 4. Natural fungal grain wood is formed under natural conditions, and the size and shape of the wood are unpredictable, and the beautiful lines may be lost during processing 5. Natural fungus wood is formed under natural conditions and is susceptible to insect pests or other fungal infections, making the wood incomplete or forming unsightly decay or discoloration

Method used

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  • Daldinia childiae and application thereof in preparation of bacterium grain wood
  • Daldinia childiae and application thereof in preparation of bacterium grain wood
  • Daldinia childiae and application thereof in preparation of bacterium grain wood

Examples

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preparation example Construction

[0062] The preparation method may also include a post-treatment step of scraping the hyphae on the surface of the mycelium wood obtained after inoculation and culture, washing and drying to a moisture content of 8-10%.

[0063] The components of PDA liquid medium are calculated in g / L as: potato 160~240, glucose 10~20, natural pH.

[0064] The components of the PDA liquid medium in g / L are preferably: potato 200, glucose 15, pH 6.5-7.0.

[0065] The wood-based material in step B is any one or more of logs, solid wood blocks, wood boards, veneers, wood chips, wood sticks, and special-shaped wood products.

[0066] The tree species of the wood-based material in step B can be any tree species.

[0067] The tree species of the wood-based material described in step B is preferably selected from the species of Populus, Alder, Betula, Rosaceae, Balsa, Bombax, or Quercus of Fagaceae.

[0068] The tree species of the wood-based material described in step B is more preferably from Populus tomentos...

Embodiment 1

[0079] —— Xylaria spp ( Daldinia childiae ) Acquisition, identification and preservation of L2Ma.

[0080] (1) Xylaria verticillata fungi ( Daldinia childiae ) Acquisition and identification of L2Ma.

[0081] The Xylaria verticillata fungus of the present invention is obtained from decaying tree stumps, fallen wood or dead branches of the genus Cherry. The sample was collected from a broad-leaved woodland near Jindian Park in Kunming City, Yunnan Province, and 100g of a decaying stump sample of the genus Prunus with black lines in the xylem was collected, together with the semicircular fungal fruiting bodies on it.

[0082] Cut the collected decayed tree stump samples with a scalpel into small pieces of 0.3~0.5cm×0.3~0.5cm×0.3~0.5cm, soak and disinfect with 75% alcohol for 10~15s, and then soak with 0.1% mercury for 5min , And then rinse 3 times with sterile distilled water. Then place it in an enrichment medium at 26°C±2°C in the dark, and stand still for 10 days to grow hyphae; t...

Embodiment 2

[0094] ——From Xylaria fungi ( Daldinia childiae ) L2Ma preparation of mycelium wood (liquid method).

[0095] (1) Preparation of mycelium wood.

[0096] The L2Ma strain of the present invention Daldinia childiae , CGMCC No: 5954, inoculated on PDA liquid medium, cultured on a dark shaker at 26℃±2℃ for 9 days to form a mycelium with a diameter of 0.1~3cm and a suspension of mycelial spores.

[0097] The components of PDA liquid medium in g / L are: potato 200, glucose 17, pH6.5~7.0.

[0098] Then, the Southwest birch logs to be inoculated were sawn into square blocks of 2cm×2cm×2cm (12 pieces in total), and placed in a culture flask with a bottom diameter of 6cm and a height of 11cm. Next, pour the vermiculite into the culture flask to cover the wood block, add a small amount of water to moisten the wood block and vermiculite, but no excess water overflows, cover the bottle cap and place it in a high-pressure steam sterilizer at 121°C Sterilize for 40 minutes.

[0099] Clamp the wood blo...

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Abstract

The invention discloses Daldinia childiae L2Ma and application thereof in preparation of bacterium grain wood, belonging to the technical field of microorganisms. The strain is collected in China General Microbiological Culture Collection Center; the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing City; and the collection number is CGMCC No:5954. The strain has excellent impregnation capability for a wood-based material, the impregnation time is short, and the impregnation depth is large; beautiful colors and grains can be formed on the surface of the material; and the physical mechanical strength is not influenced. A method for preparing a bacterium grain wood by using the strain comprises the steps of strain activation, expanding propagation and inoculation culture; and the method comprises the following specific steps: inoculating the strain on an activation and expanding propagation culture medium, performing activation and expanding propagation at 22-30 DEG C for 7-15 days, sterilizing the wood-based material, enabling the wood-based material to be sufficiently in contact with the strain subjected to activation and expanding propagation, and culturing at 22-30 DEG C for 6-8 weeks. According to the invention, the method is reasonable in process and simple to operate; and the prepared bacterium grain wood has favorable decoration effect and has no influence on the physical mechanical property of the base material, thereby being beneficial to large-scale production and popularization.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a fungus strain of the genus Anthroporum which has a short infection time and large depth, beautiful color and texture, and has no influence on the physical and mechanical strength of wood-based materials and its use in the preparation of fungus-grained wood. Applications. Background technique [0002] The fungal discoloration of wood has long been regarded as a defect of wood, which reduces the value of wood. In fact, fungal discoloration, such as lines and stains, has natural and unique characteristics. When the decay is not serious A wonderful touch. If this kind of wood with natural lines or colors is used to make handicrafts, such as vases, earrings, pens, wood veneers, etc., the added value of wood can be increased. The fungus wood with natural patterns and colors will be popular in the market, especially in the decoration, decoration and handicraft markets. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H15/00B27K5/00
Inventor 邱坚何海珊罗蓓伍建玲伍建榕甘昌涛
Owner SOUTHWEST FORESTRY UNIVERSITY
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