Daldinia childiae and application thereof in preparation of bacterium grain wood
A technology of charcoal horn bacteria and fungi, applied in the field of microorganisms, can solve the problems of inability to process and use, excessive inhalation, incomplete wood, etc., and achieves the advantages of large-scale production and popularization and application, reasonable technical route, and operation process. simple effect
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[0062] The preparation method may also include a post-treatment step of scraping the hyphae on the surface of the mycelium wood obtained after inoculation and culture, washing and drying to a moisture content of 8-10%.
[0063] The components of PDA liquid medium are calculated in g / L as: potato 160~240, glucose 10~20, natural pH.
[0064] The components of the PDA liquid medium in g / L are preferably: potato 200, glucose 15, pH 6.5-7.0.
[0065] The wood-based material in step B is any one or more of logs, solid wood blocks, wood boards, veneers, wood chips, wood sticks, and special-shaped wood products.
[0066] The tree species of the wood-based material in step B can be any tree species.
[0067] The tree species of the wood-based material described in step B is preferably selected from the species of Populus, Alder, Betula, Rosaceae, Balsa, Bombax, or Quercus of Fagaceae.
[0068] The tree species of the wood-based material described in step B is more preferably from Populus tomentos...
Embodiment 1
[0079] —— Xylaria spp ( Daldinia childiae ) Acquisition, identification and preservation of L2Ma.
[0080] (1) Xylaria verticillata fungi ( Daldinia childiae ) Acquisition and identification of L2Ma.
[0081] The Xylaria verticillata fungus of the present invention is obtained from decaying tree stumps, fallen wood or dead branches of the genus Cherry. The sample was collected from a broad-leaved woodland near Jindian Park in Kunming City, Yunnan Province, and 100g of a decaying stump sample of the genus Prunus with black lines in the xylem was collected, together with the semicircular fungal fruiting bodies on it.
[0082] Cut the collected decayed tree stump samples with a scalpel into small pieces of 0.3~0.5cm×0.3~0.5cm×0.3~0.5cm, soak and disinfect with 75% alcohol for 10~15s, and then soak with 0.1% mercury for 5min , And then rinse 3 times with sterile distilled water. Then place it in an enrichment medium at 26°C±2°C in the dark, and stand still for 10 days to grow hyphae; t...
Embodiment 2
[0094] ——From Xylaria fungi ( Daldinia childiae ) L2Ma preparation of mycelium wood (liquid method).
[0095] (1) Preparation of mycelium wood.
[0096] The L2Ma strain of the present invention Daldinia childiae , CGMCC No: 5954, inoculated on PDA liquid medium, cultured on a dark shaker at 26℃±2℃ for 9 days to form a mycelium with a diameter of 0.1~3cm and a suspension of mycelial spores.
[0097] The components of PDA liquid medium in g / L are: potato 200, glucose 17, pH6.5~7.0.
[0098] Then, the Southwest birch logs to be inoculated were sawn into square blocks of 2cm×2cm×2cm (12 pieces in total), and placed in a culture flask with a bottom diameter of 6cm and a height of 11cm. Next, pour the vermiculite into the culture flask to cover the wood block, add a small amount of water to moisten the wood block and vermiculite, but no excess water overflows, cover the bottle cap and place it in a high-pressure steam sterilizer at 121°C Sterilize for 40 minutes.
[0099] Clamp the wood blo...
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