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Method for producing agilawood

A kind of agarwood, agarwood tree technology, applied in the field of microorganisms, can solve the problems of bacterial death, low yield of agarwood, affecting the synergy of strains, etc., to achieve the effect of convenient promotion

Active Publication Date: 2015-07-15
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the artificial inoculation method has achieved certain effects and is easy to operate, the selected bacteria are not the best combination. When they are inserted into the trunk, there will be competition for survival, which may cause the death of some types of bacterial strains and affect the growth rate of the bacterial strains. The synergistic effect between them makes the yield of agarwood not high enough

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: prepare the microcapsule of wrapping mixed strain of the present invention

[0033]Rhizoctonia sp. (CPCC480164), Fusarium oxysporum (CPCC810100), Acremonium strictum (ACCC30554), Acremonium endophytium (CCTCCM206118), Penicillium sp. (CPCC441453) Each bacterial strain was inoculated in potato liquid culture medium, and cultured in a shake flask at 25°C until the bacterial cells grew vigorously to obtain a culture solution. Acremonium furcatum (CGMCC3.5370) was inoculated in potato liquid medium, and cultured in a shake flask at 27°C until the bacteria grew vigorously to obtain a culture solution.

[0034] The culture solution of the above bacterial strains was mixed with 3% sodium alginate (mass fraction) solution at a volume ratio of 1:30, and then slowly dropped into an equal volume of 4.5% calcium chloride solution, slowly stirred, and vacuum-dried to obtain each For the microcapsules of strains, equal volumes of the microcapsules of each strain are mi...

Embodiment 2

[0035] Embodiment 2: prepare the microcapsule of wrapping mixed strain of the present invention

[0036] Rhizoctonia sp. (CPCC480164), Fusarium oxysporum (CPCC810101), Acremonium strictum (ACCC30554), Acremonium endophytium (CCTCCM206118), Penicillium sp. (CPCC441453) Each bacterial strain was inoculated in potato liquid culture medium, and cultured in a shake flask at 25°C until the bacterial cells grew vigorously to obtain a culture solution. Acremonium furcatum (CGMCC3.5370) was inoculated in potato liquid medium, and cultured in a shake flask at 27°C until the bacteria grew vigorously to obtain a culture solution.

[0037] The culture solution of the above bacterial strains was mixed with 3% sodium alginate (mass percentage) solution at a volume ratio of 1:30, and then slowly dropped into an equal volume of 4.5% calcium chloride solution, slowly stirred, and vacuum-dried to obtain each For the microcapsules of strains, equal volumes of the microcapsules of each strain are...

Embodiment 3

[0038] Embodiment 3: Agarwood produced by artificial inoculation method and test comparison

[0039] 1. The inventive method

[0040] Choose the 3-year-old white wood fragrant tree. At 5 cm from the top of the tree, the vertical trunk uses a common electric drill. The diameter of the drill bit is 0.6 cm. Drill 5 deep 4 cm holes, and insert the microcapsules prepared by 5 g of Example 1 in each hole. , then seal the hole with a rubber stopper. After 6 months, the tan oily matter formed around the wound and the yellowish brown discolored xylem were harvested and dried to obtain agarwood.

[0041] 2. Comparison method

[0042] Select a 3-year-old white wood fragrant tree, and use an ordinary electric drill for the vertical trunk at a distance of 5cm from the top of the tree. The diameter of the drill bit is 0.6cm, and 5 holes with a depth of 4cm are drilled. According to the method recorded in the specific implementation of patent 201210197582.8, the mixed strain Inject into t...

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PUM

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Abstract

The invention relates to the field of microorganisms and discloses a method for producing agilawood. The method comprises the following steps: drilling holes in a trunk or branches of an agilawood tree or aquilaria sinensis tree; plugging microcapsules wrapped by mixed strain such as Rhizoctonia, Fusarium oxysporum, Acremonium strictum, dense Acremonium strictum, endogenous Acremonium strictum and Penicillium into holes; then, sealing the holes; adding the microcapsules once at an interval of one to two months; after processing for 3-24 months, cutting sepia oily substances and yellowish-brown color-changing xylem parts in the trunk; and air-drying to obtain the agilawood. According to the method disclosed by the invention, the existing mixed strain for stimulating formation of the agilawood is wrapped and placed in the agilawood tree or aquilaria sinensis tree through a sodium alginate-calcium chloride system, the strain is released as well as grows and propagates in the trunk body to stimulate the trunk or the branches to generate the agilawood together, and both the content of alcohol-soluble extract and agilawood oil in the agilawood and the content of sesquiterpenes in the agilawood oil are higher than those of an existing method.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for producing agarwood. Background technique [0002] Agarwood is the xylem of Aquilaria agallocha Roxb. or Aquilaria sinensis (Lour.) Gilg.] containing black resin. The former is mainly produced in India, Malaysia and other places, and our country calls it imported agarwood, while the latter is mainly produced in Hainan, Guangdong, Guangxi and other provinces and regions in my country, and is called domestic agarwood or soil agarwood. Agarwood is not only a traditional precious medicinal material in my country, Japan, India and other Southeast Asian countries, but also a precious natural spice. Agarwood has a history of at least 3,000 years in the Middle East, China, and Japan. It is also recorded in India and France, and even in the Old Testament of the Bible. At present, agarwood is still one of the most expensive spices in the world, and the value of agarwood exported...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06
CPCA01G7/06A01G18/10
Inventor 王军戴好富梅文莉王辉李薇
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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