Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of method of producing agarwood

A kind of agarwood and strain technology, applied in the field of microorganisms, can solve the problems of strain death, affecting the synergy of strains, and insufficient yield of agarwood

Active Publication Date: 2018-01-26
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the artificial inoculation method has achieved certain effects and is easy to operate, the selected bacteria are not the best combination. When they are inserted into the trunk, there will be competition for survival, which may cause the death of some types of bacterial strains and affect the growth rate of the bacterial strains. The synergistic effect between them makes the yield of agarwood not high enough

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Preparation of microcapsules coated with mixed strains of the present invention

[0033] Rhizoctonia sp. (CPCC480164), Fusarium oxysporum (CPCC810100), Acremonium strictum (ACCC30554), Acremonium endophytium (CCTCCM206118), Penicillium sp. (CPCC441453) Each strain was respectively inoculated into potato liquid medium, and cultured in a shake flask at 25°C until the bacteria grew vigorously, and the culture solution was obtained. Acremonium furcatum (CGMCC3.5370) was inoculated into a potato liquid medium, and cultured in a shake flask at 27°C until the bacteria grew vigorously to obtain a culture solution.

[0034] The culture solution of the above-mentioned strains was mixed with 3% sodium alginate (mass fraction) solution in a volume ratio of 1:30, and then slowly dropped into an equal volume of 4.5% calcium chloride solution, stirred slowly, and dried under vacuum to obtain each For microcapsules of bacterial species, the microcapsules of various bacterial spe...

Embodiment 2

[0035] Example 2: Preparation of microcapsules coated with mixed strains of the present invention

[0036] Rhizoctonia sp. (CPCC480164), Fusarium oxysporum (CPCC810101), Acremonium strictum (ACCC30554), Acremonium endophytium (CCTCCM206118), Penicillium sp. (CPCC441453) Each strain was inoculated into potato liquid medium, and cultured in a shake flask at 25°C until the cells grew vigorously, and the culture solution was obtained. Acremonium furcatum (CGMCC3.5370) was inoculated into a potato liquid medium, and cultured in a shake flask at 27°C until the bacteria grew vigorously to obtain a culture solution.

[0037] The culture broth of the above-mentioned strains were mixed with 3% sodium alginate (mass percentage) solution in a volume ratio of 1:30, and then slowly dropped into an equal volume of 4.5% calcium chloride solution, stirred slowly, and dried in vacuum to obtain each For microcapsules of bacterial species, the microcapsules of various bacterial species are mixed in e...

Embodiment 3

[0038] Example 3: Artificial inoculation method to produce agarwood and test comparison

[0039] 1. The method of the invention

[0040] Choose a three-year-old Baimuxiang tree, at a distance of 5cm from the top of the tree, use an ordinary electric drill for the vertical trunk. The drill has a diameter of 0.6cm and drills 5 holes with a depth of 4cm. Each hole is filled with 5g of the microcapsules prepared in Example 1. , Then seal the hole with a rubber plug. Six months later, the brown oily substance and yellow-brown discolored xylem formed around the wound were harvested and dried to obtain agarwood.

[0041] 2. Comparison method

[0042] Choose a three-year-old tree of Baimuxiang tree, at a distance of 5cm from the top of the tree, use an ordinary electric drill for the vertical trunk. The drill has a diameter of 0.6cm and drills 5 holes with a depth of 4cm. Inject it into the hole by bottle insertion, and then seal the hole with a rubber stopper. After 6 months, the tan oily...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of microorganisms and discloses a method for producing agilawood. The method comprises the following steps: drilling holes in a trunk or branches of an agilawood tree or aquilaria sinensis tree; plugging microcapsules wrapped by mixed strain such as Rhizoctonia, Fusarium oxysporum, Acremonium strictum, dense Acremonium strictum, endogenous Acremonium strictum and Penicillium into holes; then, sealing the holes; adding the microcapsules once at an interval of one to two months; after processing for 3-24 months, cutting sepia oily substances and yellowish-brown color-changing xylem parts in the trunk; and air-drying to obtain the agilawood. According to the method disclosed by the invention, the existing mixed strain for stimulating formation of the agilawood is wrapped and placed in the agilawood tree or aquilaria sinensis tree through a sodium alginate-calcium chloride system, the strain is released as well as grows and propagates in the trunk body to stimulate the trunk or the branches to generate the agilawood together, and both the content of alcohol-soluble extract and agilawood oil in the agilawood and the content of sesquiterpenes in the agilawood oil are higher than those of an existing method.

Description

Technical field [0001] The invention relates to the field of microorganisms, in particular to a method for producing agarwood. Background technique [0002] Agarwood is a xylem containing black resin in Aquilaria agallocha Roxb. or Aquilaria sinensis (Lour.) Gilg. of the Thymelaeaceae family. The former is mainly produced in India and Malaysia, which is called imported agarwood in my country, while the latter is mainly produced in my country's provinces such as Hainan, Guangdong and Guangxi, and is called domestic agarwood or agarwood. Agarwood is not only a traditional precious medicinal material in my country, Japan, India and other Southeast Asian countries, but also a precious natural spice. Agarwood has a history of at least 3,000 years in the Middle East, China, and Japan. It is also recorded in India and France, and even in the Old Testament books of the Bible. At present, agarwood is still one of the most expensive spices in the world. Singapore alone exports more than ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/06
CPCA01G7/06A01G18/10
Inventor 王军戴好富梅文莉王辉李薇
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com