Method for separating bark rot pathogenic bacteria from lignum aquilarae by using host substrate

Abstract

The invention relates to a method for separating bark rot pathogenic bacteria from lignum aquilarae by using a host substrate. The method comprises the following steps: (1) collecting an onset samplefrom a lignum aquilarae planting field; (2) pretreatment of a lignum aquilarae host substrate: cutting lignum aquilarae branches into pieces, soaking the cut lignum aquilarae branch pieces, and filtering off moisture; (3) test tube split-charging: loading the cut branch pieces to test tubes, and sterilizing the loaded test tubes in a high-pressure steam sterilizing pot for later use; (4) separation of stem pathogenetic fungi of lignum aquilarae: shearing tissue pieces of a diseased-healthy joint of diseased sample tissue, loading the sheared tissue pieces into the test tubes, and carrying outculture in a constant-temperature incubator; (5) purification on a potato dextrose agar culture medium: picking mycelium pieces of growth tail ends, and culturing the picked mycelium pieces on the culture medium, so as to obtain a pure culture; (6) pathogenicity assaying; and (7) pathogenic bacterium morphology observation. According to the method, by taking the host substrate as the culture medium, propagation of bacteria can be prevented, pathogenic bacterium mycelia with relatively high growth speed can exceed microbes with relatively low growth speed and are effectively separated from hybrid microbes, and thus, pure strains are obtained.

Description

technical field [0001] The invention relates to a method for isolating pathogenic bacteria of agarwood bark rot by using a host matrix, belonging to the field of plant protection. Background technique [0002] Aquilaria sinensis (Lour.) Spreng, the scientific name is Aquilaria sinensis (Lour.) Spreng, which is a perennial tree belonging to the family Daphneaceae, and is a national second-class endangered plant. Its product agarwood is a traditional precious medicinal material and natural spice with high economic value and great market demand. In recent years, with the improvement of the economic value of agarwood trees, the planting area of ​​agarwood trees has gradually expanded, and the subsequent diseases of agarwood trees have also gradually increased. [0003] The pathogenic bacteria of the agarwood bark rot grow on the outer bark of the agarwood tree, and the outer bark is exposed to the air for a long time. In addition to the pathogenic bacteria, there are also many ...

AI Technical Summary

Problems solved by technology

Using conventional tissue separation methods for surface disinfection treatment can easily cause the loss of activity of pathogenic bacteria growing on the outer skin layer, making it difficult to isolate pathogenic bacteria

[0004] At present, there are few related reports on the pathogenic bacteria of agarwood rot, and even fewer of them have been isolated. Therefore, in order to better study the related situation of agarwood rot, it is of great significance to study and isolate the pathogenic bacteria of agarwood rot

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