Method for quickly and efficiently propagating Callicarpa japonica
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A purple bead, fast technology, applied in horticultural methods, botanical equipment and methods, planting substrates, etc., can solve the problem of poor rooting quality of Japanese purple bead, improper coordination of tissue culture methods and culture substrates, and low survival rate of tissue culture seedlings. and other problems, to achieve the effect of promoting rapid reproduction and promotion, improving the quality of tissue culture seedlings, and shortening the breeding cycle.
Active Publication Date: 2021-07-23
CHONGQING ACADEMY OF FORESTRY SCI
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[0005] In view of this, the purpose of the present invention is to provide a method for rapid and efficient propagation of Japanese purple pearls, to solve the problems that the tissue culture method and the culture medium are improperly matched, resulting in poor rooting quality of Japanese purple pearls and low survival rate of tissue cultured seedlings
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Embodiment 1
[0039] Before the rapid and efficient propagation of Japanese purple pearls, the gel nutrition tablets used in the strong seedling and rooting culture stages were prepared. The preparation method is as follows:
[0040] First weigh 30g of Sago starch, 10g of sodium lignosulfonate, 15g of pectin, 5g of sodium trimetaphosphate, and 8g of potassium dihydrogen phosphate.
[0041] Preparation:
[0042] Sago starch modification: adjust Sago starch into starch milk with a mass fraction of 45%, add sodium lignosulfonate, stir at a speed of 900r / min for 15min, and stand at room temperature for 12h overnight;
[0043] Gelatinization modification: Stir the modified Sago starch evenly after standing still, heat in a water bath at 40°C for 20 minutes, add pectin and stir for 15 minutes, stop heating after stirring, and make potassium dihydrogen phosphate into a saturated solution , add to the starch solution and stir evenly, then add sodium trimetaphosphate, stir evenly, heat to 75°C for ...
Embodiment 2
[0046] First weigh 40g of Sago starch, 12g of sodium lignosulfonate, 16g of pectin, 8g of sodium trimetaphosphate, and 10g of potassium dihydrogen phosphate.
[0047] The preparation method is the same as in Example 1.
Embodiment 3
[0049] First, weigh 50 g of Sago starch, 15 g of sodium lignosulfonate, 18 g of pectin, 10 g of sodium trimetaphosphate, and 12 g of potassium dihydrogen phosphate.
[0050] The preparation method is the same as in Example 1.
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Abstract
The invention discloses a method for quickly and efficiently propagating Callicarpa japonica, and belongs to the technical field of Callicarpa japonica propagation and cultivation. The propagation method comprises the following steps: (1) explant preparation: selecting tender shoots, which just germinates in spring,of Callicarpa japonica as explants; (2) aseptic treatment of the explants: stripping all tissues except true leaves on the tender shoots for disinfection treatment; (3) primary culture: transferring the disinfected tender shoots into a primary culture medium; (4) subculture: after new contact surfaces are exposed, transferring the whole shoots into a subculture medium, and transferring for three times to form clumpy buds; and (5) integrated culture of seedling strengthening and rooting outside a bottle: cutting subculture cluster buds with strong growth vigor into single buds, putting a matrix and a gel nutrient sheet into hole trays, inserting the single buds to be cut into the matrix by about 1 cm by using tweezers, then thoroughly watering the single buds by using a rooting solution, and completely covering the single buds by using a transparent film. The problems that the rooting quality of the Callicarpa japonica is poor and the survival rate of tissue culture seedlings is low due to improper matching of a tissue culture method and a culture medium are solved, meanwhile, the culture period is shortened, and the culture cost is reduced.
Description
technical field [0001] The invention relates to the technical field of Japanese purple pearl breeding and cultivation, in particular to a method for rapid and efficient propagation of Japanese purple pearl. Background technique [0002] Japanese purple bead, scientific name: Callicarpa japonica Thunb.var.japonica, Verbenaceae purple bead is a deciduous shrub, English name beautyberry, known as beautiful berry. The inflorescence is cymose, lavender, the branches are soft and fluffy, the plant shape is fluffy, the autumn fruit is purple, with luster, very showy, and each node grows in continuous clusters, which will not fall for a long time. It has high ornamental value. Studies have shown that Zizhu leaves also have good medicinal value. Zizhu fruit extract has a variety of excellent antioxidant components, which can inhibit the formation of stains and resist skin aging. [0003] The wild species of Japanese purple pearl is more differentiated, and horticultural species are ...
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