Application of sulfated codonopsis pilosul polysaccharide to preparation of herpes simplex virus type I resistance medicament and preparation method of herpes simplex virus type I resistance medicament
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A technology of herpes simplex virus and Codonopsis polysaccharide, applied in the field of pharmacy
Inactive Publication Date: 2014-11-19
ZUNYI MEDICAL UNIVERSITY
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However, in the literature of Codonopsis anti-virus research, only Gou Peng reported that Codonopsis verticillum extract has a certain inhibitory effect on canine parvovirus, and Codonopsis polysaccharide sulfate has an inhibitory effect on chicken Newcastle disease virus (NDV)
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Embodiment 1
[0085] Embodiment 1 of the present invention: the preparation method of Codonopsis polysaccharide sulfate ester, specifically as follows:
[0086] 1) Extraction of Codonopsis polysaccharide
[0087] Decoction with water - alcohol precipitation method. Take 1000 g of Dangshen decoction pieces, crush them, put them in a Soxhlet extractor, heat and reflux with 80% ethanol for 3 times to degrease, add 10 times the amount of water to decoct the dregs for 3 times, the first time for 3 hours, the second time for 2 hours, and the second time for 2 hours. 3 times for 1 h; the residue was filtered with suction, and the filtrates were combined for 3 times and then concentrated. Add 95% ethanol to the concentrated solution until the ethanol content is 80%, put it in a refrigerator at 4 °C for 12 h, centrifuge at 3000 r / min, collect the precipitated part, dry it in vacuum, redissolve, add 95% ethanol until the ethanol content is 80% %, centrifuge, collect the precipitate, freeze-dry, and...
Embodiment 2
[0097] Embodiment 2 of the present invention: Take Codonopsis Polysaccharide Sulfate 3g , medical starch 75 g, microcrystalline cellulose 20 g, the medicinal starch is dried first, passed through a 120 mesh sieve, and then mixed with Codonopsis polysaccharide sulfate , microcrystalline cellulose are mixed, cross 120 mesh sieves twice, fill in the hard capsule, make 1000 capsules of the present invention. Each hard capsule contains the main ingredient 0.3 mg.
Embodiment 3
[0098] Embodiment 3 of the present invention: Take Codonopsis Polysaccharide Sulfate 3g , 6 g of hypromellose, 10 g of sodium starch glycolate, 8 g of microcrystalline cellulose, 115 g of lactose, 50 g of starch, and 1 g of magnesium stearate; fully mix the main ingredient and auxiliary materials and put them into a high-speed mixer, spray with appropriate amount of water, Whole grains, the water content is controlled at 3~4%, and then pressed into tablets to make 1000 tablets, coated with film. Each capsule contains main ingredients 0.3 mg.
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Abstract
The invention discloses application of sulfated codonopsis pilosul polysaccharide to preparation of a herpes simplex virus type I resistance medicament and a preparation method of the herpes simplex virus type I resistance medicament. According to the invention, a sulphamic acid method is adopted to carry out sulphating modification on codonopsis pilosul polysaccharide, a modification method is optimized and an excellent prevention and treatment effect of codonopsis pilosul polysaccharide on the herpes simplex virus type I is found. Meanwhile, the sulfated codonopsis pilosul polysaccharide (the substituted ratio is 0.763) with the best activity is also screened out; no matter prevention administration or treatment administration, the cell survival rate of the sulfated codonopsis pilosul polysaccharide is higher than that of a virus control group (P is smaller than 0.01), the prevention effect of the sulfated codonopsis pilosul polysaccharide is equivalent to that of acyclovir (P is greater than 0.05) and the treatment effect of the sulfated codonopsis pilosul polysaccharide is superior to that of acyclovir (P is smaller than 0.05). The effect that the antiviral activity of the sulfated codonopsis pilosul polysaccharide is the highest is shown; the sulfated codonopsis pilosul polysaccharide can be used as a material for developing the novel HSV-I ( herpes simplex virus type I) resistance medicament and the material and theoretical foundation are provided for development of the novel HSV-I resistance medicament.
Description
technical field [0001] The invention relates to the field of pharmacy, in particular to the application of codonopsis polysaccharide sulfate in the preparation of anti-herpes simplex virus type I drugs and its preparation method. Background technique [0002] Herpes simplex virus type Ⅰ (herpes simplex virus type Ⅰ, HSV-Ⅰ) belongs to the herpes virus subfamily, mainly causing skin, mucous membrane (oral mucosa) and organ (brain) infections other than the genitals, and can cause human mucosal and nervous system infections above the waist. Infection, the resulting diseases mainly include keratitis, herpetic encephalitis, herpes labialis and gingivostomatitis, etc., and can form latent infection. Humans are the only natural host of HSV. The virus enters the body through the respiratory tract, oral cavity, genital mucous membrane and damaged skin, and resides in the normal mucous membrane, blood, saliva and sensory ganglion cells of the human body. Most of the primary infectio...
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