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The present application relates to a kind of anticoagulation, anti-oxidative stress hemodialysis apparatus and preparation method, belong to biomedical polymer and hemodialysis field.Oxygen-containing functional group carbon dots, anticoagulation monomer, initiator and crosslinking agent are added into water, to obtain modified liquid of anticoagulation and anti-oxidation;Protective gas is introduced into hemodialysis apparatus to remove oxygen, then modified liquid is introduced into dialysis apparatus, then sealing heating is carried out to initiate anticoagulation monomer in situ polymerization on the surface of dialysis membrane, and the oxygen-containing functional group carbon dots form gel microspheres under the wrapping of polymer, i.e., anticoagulation, anti-oxidative stress hemodialysis apparatus is obtained.The APTT of modified dialysis apparatus can reach 600s, and the removal rate of DPPH and ABTS free radicals is 71.25% and 97.47% respectively.The present application only needs to carry out one-step surface modification to dialysis apparatus finished product, which can give hemodialysis apparatus the dual functions of anticoagulation and anti-oxidative stress, is simple and convenient, and has good application prospect.