Stichopus japonicus step-by-step hybrid breeding method

Abstract

The present invention discloses a stichopus japonicus grading breeding method. It is characterized by that said method includes the following steps: making sex glands of Chinese stichopus japonicus and Chinese-Russian hybrid stichopus japonicus be simultaneously developed and matured, using shade-drying parturifacient method to respectively obtain sperm of Chinese stichopus japonicus and ovum of Chinese-Russian hybrid stichopus japonicus, making artificial insemination and incubating fertilized ovum until the laval plankton is formed, after the laval plankton is attached, utilizing conventional method to breed F1 generation of stichopus japonicus of Chinese stichopus japonicus and Chinese-Russian stichopus japonicus, then using said F1 generation stichopus japonicus as seed stichopus japonicus, accelerating the sex glands of said F1 generation stichopus japonicus and Chinese stichopus japonicus to make them be synchronously matured, making artificial insemination and breeding so as toobtain a new young of stichopus japonicus.

Description

Technical field: [0001] The invention relates to a sea cucumber breeding method, in particular to a stepwise hybrid breeding method using Chinese sea cucumbers and Chinese-Russian hybrid sea cucumbers as parents. Background technique: [0002] Apostichopus japonicus is a famous and valuable seafood, rich in protein and viscous polysaccharides, with high nutritional health and medicinal value, popular with consumers, high price, and large market capacity. In recent years, with the gradual expansion of the consumption demand of sea cucumbers, the scale of artificial cultivation of sea cucumbers has become larger and larger, and the cultivation methods are various. [0003] In the past, sea cucumber seedlings were mainly obtained by harvesting wild seedlings or artificial seedlings. Among them, artificial seedlings were the main source. After natural maturation or artificial maturation, artificial fertilization and incubation were carried out to cultivate larvae, immature and j...

AI Technical Summary

Problems solved by technology

[0005] However, the seed dominance of Chinese-Russian hybrid sea cucumbers is generally only shown in the F1 generation, and the traits segregate from the self-crossed F2 generation. The individual differences in the F2 generation group are very large, the growth is irregular, and the growth vigor and stress resistance are not uniform. Compared with the F1 generation, the sex and yield were significantly lower than that of the F1 generation, so that the dominance of the F1 generation declined gradually; at the same time, the selfing of the F1 generation would inevitably lead to the homozygosity of alleles and the recessive and harmful traits. The F2 generation and subsequent generations of ginseng selfing are generally no longer used in production.

[0006] Although the theory and practice of genetic breeding have proved that the breeding of progressive hybridization (also known as modified hybridization and absorption hybridization) has the advantages of improving the stress resistance and vitality of the cultured objects, but due to the asynchronous development of the gonads of the improved sea cucumber in China In addition, sea cucumbers are dioecious, and it is difficult to distinguish them from the appearance, so the current progressive hybridization is limited to mammals (meat dogs, sheep, cattle, etc.), and there is no stepwise hybridization of sea cucumbers in existing publications. any reports of