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Method for improving mating ability of male Wistar rats

A male and capable technology, applied in the field of Chinese herbal medicine extract formulations for improving the mating ability of rats, can solve the problems of difficulty in the production of experimental animals, low incidence of spontaneous tumors, and high prices, and can reduce the latency of capturing and climbing. , the effect of shortening the breeding cycle and low cost

Inactive Publication Date: 2021-06-18
YANGZHOU UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

④ Low incidence of spontaneous tumors
However, Chinese medicinal materials such as ginseng and deer antler are expensive and often counterfeited, and are not easy to be used in experimental animal production. Therefore, it is of great value and significance to screen natural plant Chinese medicinal materials with low prices and good medicinal effects.

Method used

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  • Method for improving mating ability of male Wistar rats
  • Method for improving mating ability of male Wistar rats
  • Method for improving mating ability of male Wistar rats

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Embodiment Construction

[0023] The present invention will be further described below in conjunction with accompanying drawing:

[0024] After drying the four kinds of Chinese herbal medicines Cistanche, Cynomorium, Epimedium and Lycium barbarum, crush them to 150 mesh with a pulverizer; weigh a certain amount of Chinese herbal medicine powder, and soak them in 75% medical ethanol according to the mass ratio of 1:10 , sealed at 23±2°C for 24 hours and then suction filtered, the filter residue was soaked in 75% medical ethanol, repeated suction filtration 3 times, and combined the filtrate; the above filtrate was concentrated under reduced pressure using a rotary evaporator, and the temperature of Cistanche and Cynomorium was set at 65 ℃, the temperature of Epimedium medlar is set at 60°C, and the speed is 60rpm. After being concentrated into an extract, place it in an oven to evaporate the residual water; use distilled water to dilute the four traditional Chinese medicines of Cistanche, Cynomorium, Epi...

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Abstract

The invention discloses a method for improving mating ability of male Wistar rats, and belongs to the technical field of experimental animal production. The method is characterized in that four traditional Chinese medicine extracts of herba cistanche, herba cynomorii, herba epimedii and fructus lycii are combined according to the concentration ratio of 0.5 mg / mL, 1.0 mg / mL, 0.6 mg / mL and 1.4 mg / mL, the male Wistar rats are subjected to intragastric administration every day, the mating ability of the male Wistar rats in a short period is obviously enhanced, the rat catching and climbing times are effectively increased, the catching and climbing incubation period is obviously reduced, the breeding period is greatly shortened, the application values of low cost, short time consumption, simple operation and the like in the aspects of laboratory rat breeding, breeding system establishment and the like are achieved, and the method has practical reference basis and wide application prospect in the aspects of breeding protection of endangered mammals, treatment of erectile dysfunction of male animals, development of new drugs and the like.

Description

technical field [0001] The invention belongs to the technical field of experimental animal production, and relates to a method for improving the mating ability of male rats, in particular to a formula of Chinese medicinal material extracts for improving the mating ability of rats. Background technique [0002] Wistar rats are white closed group rats, which were bred by the Wistar Institute in the United States in 1907, and have now spread to laboratories all over the world. Its main features are: ①The head is wider, the ears are longer, and the length of the tail is shorter than the body length. ②The sex cycle is stable, the fecundity is strong, and there are many litters. The average number of litters per litter is about 10, and the growth and development are fast. ③ Strong resistance to infectious diseases. ④ low incidence of spontaneous tumors. ⑤ It has high requirements on humidity, and it is prone to ring tail disease when the relative air humidity is lower than 40%....

Claims

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Application Information

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IPC IPC(8): A61K36/815A61P15/00A61P15/10A61K36/185A61K36/296A61K36/64
CPCA61K36/185A61K36/296A61K36/64A61K36/815A61K2236/333A61K2236/39A61P15/00A61P15/10A61K2300/00
Inventor 朱福洲魏万红余凡顾晨李全鑫
Owner YANGZHOU UNIV
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