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25results about How to "Quick cost" patented technology

SNP (Single Nucleotide Polymorphism) molecular marker related to sheep fecundity and application thereof

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to sheep fecundity and application of the SNP molecular marker. The SNP molecular marker comprises an SNP1 (Single Nucleotide Polymorphism) and / or an SNP2; the SNP1 is located at a base at the 115747587 site of a chromosome 6 of a sheep reference genome AR-UIRamV2.0 version, and the polymorphism of the SNP1 is T / G; the SNP2 is located at a basic group at the 41419566th site of a chromosome 22 of a sheep reference genome AR-UIRamV2.0 version, and the polymorphism of the SNP2 is A / G. According to the scheme, the SNP molecular marker related to sheep fecundity is provided, sheep with the high fecundity character can be efficiently and accurately identified and screened out, and the marker is high in typing quality, single in copy and high in polymorphism and can be used for sheep fecundity molecular marker assisted breeding.
Owner:HUAZHI RICE BIO TECH CO LTD +1

Zn-MOF fluorescence detection probe as well as preparation method and application thereof

The invention belongs to the technical field of chemical sensing, and particularly relates to a Zn-MOF fluorescence detection probe as well as a preparation method and application thereof. The structural unit of the Zn-MOF fluorescence detection probe is [Zn2 (TPB) (BPDC-(OH) 2) 2]. (DMF). (H2O), wherein the BPDC-(OH) 2 is a 2, 5-dihydroxy terephthalic acid anion; the fluorescence detection probe belongs to a monoclinic system, the space group is C2 / c, and the Zn-MOF fluorescence detection probe has the characteristics of good selectivity, high sensitivity, high response speed and the like. The preparation method comprises the following steps: mixing 2, 5-dihydroxyterephthalic acid, 1, 2, 4, 5-tetra (4-pyridyl) benzene, zinc nitrate, N, N-dimethylformamide and deionized water, carrying out a heating reaction, filtering, and drying to obtain the Zn-MOF fluorescence detection probe. The preparation method has the advantages of easily available raw materials, simple operation and the like. The Zn-MOF fluorescence detection probe can be used for detecting sulfasalazine, realizes visual identification of SSZ according to the change of fluorescence intensity of the probe, can qualitatively determine SSZ in real time through a portable fluorescent lamp, and has important application value.
Owner:QUFU NORMAL UNIV

Remote sensing image road extraction method and equipment based on knowledge distillation, and readable storage medium

The invention relates to the technical field of remote sensing image road extraction, and provides a remote sensing image road extraction method and device based on knowledge distillation, and a readable storage medium, and the method comprises the steps: constructing a teacher model and a student model, employing an encoder-decoder architecture to learn road features for neural networks of the teacher model and the student model, the number of layers of the student model neural network is smaller than that of the teacher model neural network, a middle layer is adopted for knowledge distillation, knowledge distillation of the middle layer is isomorphic distillation, and a result is output based on the model after target training. The problems that a traditional method is large in parameter quantity and high in calculation complexity are solved, real-time reasoning can be completed rapidly and accurately, and the requirement for real-time road extraction is met.
Owner:XIAMEN UNIV OF TECH

Method for constructing anthocyanin plant cell factory by using VdUFGT gene overexpression

The invention belongs to the field of plant gene engineering, and particularly relates to a method for constructing an anthocyanin plant cell factory by using VdUFGT overexpression, a cloned vitis davidii VdUFGT gene is subjected to autogenetic transformation to obtain a VdUFGT high-expression and anthocyanin high-yield cell line, and the cell line remarkably improves the expression of anthocyanin biosynthesis key genes, so that the yield of anthocyanin is improved. And in combination with cell suspension culture, a high-yield anthocyanin plant cell factory is constructed. According to the method, the anthocyanin yield can be increased by 8.5 times and reaches 3.17%, the anthocyanin production period can be shortened to 12 days, the method has the advantages of being low in cost, short in period, high in efficiency, environmentally friendly and the like, and large-scale annual uninterrupted production of natural anthocyanin can be achieved.
Owner:INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI

Method for enriching precious metal from carbonylation alloy

PendingCN119506590Areduce dosageImprove direct yieldPlatinumSlag
The invention discloses a method for enriching precious metal from carbonylation alloy, which comprises the following steps: S1, adding the carbonylation alloy with the granularity of 1-3mm and 2.0-2.5 mol / L hydrochloric acid solution into a mechanical kettle according to the liquid-solid ratio of (4-5): 1, and sequentially adding the carbonylation alloy with the granularity of 1mm-3mm and 2.0-2.5 mol / L hydrochloric acid solution into the mechanical kettle according to the arriving position of feed liquid; s2, sulfating roasting: controlling the ratio of the slag subjected to electric chlorination washing to concentrated sulfuric acid to be 2: 1, adding the slag to a rotary kiln, dividing the rotary kiln into three temperature sections, taking a kiln head as a preheating and drying section, and controlling the temperature to be 250-40 DEG C; an acidification roasting section is arranged in the kiln, and the temperature is 500-600 DEG C; the kiln tail is a selenium steaming section, the temperature is 600-650 DEG C, and the material roasting time is 8-10 hours; and S3, water leaching is conducted, specifically, the materials obtained after sulfuric acid roasting and water are added into an enamel water leaching kettle according to the liquid-solid ratio of (4-5): 1, after the water leaching temperature is 80-93 DEG C and the water leaching time is 0.5 h, filter pressing separation is conducted, and residues serve as platinum concentrate to enter the next purification procedure.
Owner:金川集团铜贵股份有限公司

A visible light optical system and a visible light optical lens

This application discloses a visible light optical system and a visible light optical lens. The visible light optical system includes a superlens, a first aspherical lens, a second aspherical lens, a third aspherical lens, and a fourth aspherical lens. The first, second, third, and fourth aspherical lenses are arranged sequentially along the optical axis from the object side to the image side. The superlens has a positive optical power. The first aspherical lens has a positive optical power, and both its object-side and image-side surfaces are convex towards the object side. The second aspherical lens has a negative optical power, and both its object-side and image-side surfaces are convex towards the image side. The third aspherical lens has a positive optical power, and both its object-side and image-side surfaces are convex towards the image side. The fourth aspherical lens has a negative optical power. The visible light optical system combines a superlens with a traditional refractive lens, giving it advantages such as low cost, small size, and excellent imaging quality.
Owner:湖州迈塔兰斯科技有限公司

Mechanical-electrical composite transmission hydraulic planetary mechanism speed change system

The invention provides an electromechanical composite transmission hydraulic planetary mechanism speed change system. The electromechanical composite transmission hydraulic planetary mechanism speed change system comprises a hydraulic planetary mechanism and a control mechanism, the control mechanism stores set parameters and sends a main oil way control signal and an oil pump control signal which are generated according to a vehicle starting instruction and real-time hydraulic pressure of a main oil way to the hydraulic planetary mechanism. After the main oil way is opened, upshift oil pre-charging control or upshift speed regulation control is carried out, or downshift oil pre-charging control or downshift speed regulation control is carried out, and a motor is driven to run; the hydraulic planetary mechanism opens the main oil way electromagnetic valve to the set opening degree, the real-time opening degree of the main oil way electromagnetic valve is adjusted in real time, the clutch electromagnetic valve is opened and closed, and the brake electromagnetic valve is opened and closed. The electromechanical composite transmission hydraulic planetary mechanism speed change system has the advantages of being high in control precision, high in real-time performance, fast in response, simple in structure, low in cost, small in occupied space and the like, and can be widely applied to the traffic field.
Owner:BEIJING INST OF TECH

A method for preparing functionalized fluorescent protein multicolor coded microspheres

The present invention belongs to the field of fluorescent microsphere coding technology, and specifically relates to a method for preparing functionalized fluorescent protein multicolor coded microspheres. Fluorescent protein is used instead of organic fluorescent dyes and nanomaterials, and the fluorescent protein is assembled on the surface of the microsphere by chemical coupling. The protein type, concentration and microsphere particle size are used as coding elements to obtain single-color and multicolor fluorescent microspheres with ultra-large coding capacity; the surface of the fluorescent microsphere is modified by biotin to fix streptavidin to the surface of the microsphere, and combined with biotin-labeled capture probes to achieve high-throughput rapid analysis of multiple target molecules. The preparation process of the present invention is simple, the coding capacity is large, the performance is stable, and it has strong versatility and is environmentally friendly. The microspheres of the present invention can be applied to disease diagnosis, food safety, environmental protection, proteomics, genomics and drug screening.
Owner:HUAZHONG AGRI UNIV

A method for creating diploid seedless watermelon by gene editing ClCKI1

The invention discloses a method for creating diploid seedless watermelon by gene editing ClCKI1, and belongs to the technical field of plant genetic engineering breeding. The invention discloses a method for creating diploid seedless watermelon by gene editing ClCKI1, which uses CRISPR / Cas9 technology for the first time to perform targeted editing on the ClCKI1 gene of watermelon, so that its gene function is lost, female gametes are sterile, self-pollination fruits swell normally, double fertilization cannot form zygotes, seeds are aborted, and only a small amount of white-shelled seeds are produced, thereby creating diploid seedless watermelon. Compared with wild-type materials, the edited diploid seedless watermelon plants created by the method of the invention have no obvious phenotypic changes in the vegetative growth stage and male gametes, except for the sterility of female gametes. The method has the advantages of being simple and easy to operate, high efficiency, low cost and short cycle.
Owner:NORTHWEST A & F UNIV

Adaptive Industrial Control Energy Management Scheduling System

The present invention relates to the technical field of energy optimization and scheduling, and is specifically an adaptive industrial control energy management and scheduling system, wherein the system comprises an energy demand prediction module, an energy supply strategy module, a dynamic weight adjustment module, a multi-period allocation module, a multi-objective optimization strategy module, and a real-time adjustment feedback module. The present invention improves the foresight and accuracy of energy allocation by accurately predicting future demand using historical energy consumption data, ensures the stability and economy of energy supply by optimizing the supply strategy, adopts a linear regression model to automatically adjust the energy use weight, enhances the adaptability of industrial production to energy market price fluctuations and environmental changes, adopts a Pareto frontier analysis method to balance cost, energy efficiency and environmental impact targets, realizes multi-objective optimization of energy management, can respond quickly according to real-time data, optimizes energy allocation, improves energy utilization efficiency, reduces energy waste, and reduces operating costs.
Owner:JINJIELI TECH (BEIJING) CO LTD

A robot end tool for gripping a rotary workpiece

The present invention discloses a robot end tool for gripping a rotary workpiece, wherein the main structure of the robot includes a welded part connected to the end of the robot; the power device includes a servo motor, a motor support, a synchronous pulley and a synchronous belt, the ball screw of the transmission device is provided with threads which are symmetrical in position but have different rotation directions, and a linear bearing with a seat is installed on the ball screw; the clamping jaw device includes a connecting piece and a clamping jaw for clamping the workpiece, and the clamping jaw and a slider are fixedly connected to the connecting piece; the auxiliary sensing device includes an infrared photoelectric sensor, a radar ranging sensor and a connecting plate, the infrared photoelectric sensor is used to detect the position of the connecting piece and send a signal to control the opening and closing of the clamping jaw device, the clamping jaw device can be used in conjunction with a robot for loading and unloading materials to realize machine replacement of manual operation, the clamping jaw device can grasp products of different diameters and specifications, and when producing products of different diameters and specifications, there is no need to replace another set of robot end tools, and the workpiece processing versatility is good.
Owner:TIANJIN TIANSEN INTELLIGENT EQUIP CO LTD +1

Low-cost supporting device for ground test of unmanned aerial vehicle

The utility model discloses a low-cost supporting device for unmanned aerial vehicle ground tests in the technical field of unmanned aerial vehicle ground tests, which comprises an inner frame welding assembly mounted in an outer frame welding assembly in a lifting motion manner, and a power component used for driving the inner frame welding assembly to lift is further arranged in the outer frame welding assembly. The top of the inner frame welding assembly is provided with an air spring assembly, the top of the air spring assembly is connected with a top head assembly used for being connected with the unmanned aerial vehicle, the structure is simple, the number of parts needing to be purchased is small, and therefore the manufacturing period is greatly shortened; the manufacturing cost is low, and the cost of a traditional supporting system is several times that of a supporting system designed in the invention; according to the utility model, the ground test of a certain type of unmanned aerial vehicle is successfully completed under the advantages of low cost, short period and simple installation, and the test platform has excellent practicability and can be used in unmanned aerial vehicle tests.
Owner:JIANGSU XINYANG NEW MATERIALS CO LTD

Remote sensing image road extraction method, device and readable storage medium based on knowledge distillation

The present invention relates to the technical field of road extraction from remote sensing images. The present invention provides a method, device and readable storage medium for extracting roads from remote sensing images based on knowledge distillation, which includes constructing a teacher model and a student model. The neural networks of the teacher model and the student model adopt an encoder-decoder architecture to learn road features. The number of layers of the student model neural network is less than that of the teacher model neural network. The knowledge distillation is performed in the middle layer, and the knowledge distillation in the middle layer is isomorphic distillation. The model outputs the result based on the target training. The problem of large number of parameters and high computational complexity of the traditional method is solved, and real-time reasoning can be completed quickly and accurately to meet the needs of real-time road extraction.
Owner:XIAMEN UNIV OF TECH

Slit device with dust removal function

The application discloses a slitting device with dust removal function, which comprises a machine body, a water spraying module, a slitting module and a dust removal module. The machine body is divided into upper and lower shells, and four guide wheels are arranged under the shells. The water spraying module is arranged at the front end of the machine body, a water tank is connected with a water guide pipe through a water pump, the slitting module is connected with a spring suspension at the middle part of the machine body, an oil return system is fixed on the outer wall of the suspension through a platform, an oil pump is connected with an oil tank and a reversing valve, the reversing valve is arranged at the bottom of the platform, the reversing valve is connected with an oil return cylinder and fixed on the side of the platform, the oil return cylinder is connected with the spring suspension, the two oil return cylinders of the oil return system are connected with each other, the oil return cylinder is connected with a clutch, the clutch is connected with an input and output shaft, centrifugal blocks are arranged on the output shaft, the input and output shafts are connected through bevel gears, and a belt drive is arranged between the engine and the bevel gears. The dust removal module is arranged at the end of the machine body, the engine is connected with dust removal blades of the dust removal module through a belt drive, the dust removal blades are fixed in a dust removal tank, and a filter screen is arranged on the dust removal port.
Owner:QINGDAO CIVIL ENG GRP CO LTD

A method for preparing a ruthenium / molybdenum dioxide water electrolysis hydrogen production electrode material

The invention discloses a method for preparing a ruthenium / molybdenum dioxide water electrolysis hydrogen production electrode material, comprising adding ruthenium trichloride to deionized water and stirring to obtain a first mixed solution; stirring and mixing molybdenum pentachloride with the first mixed solution to obtain a second mixed solution; stirring and mixing polyvinyl pyrrolidone with the second mixed solution to obtain a third mixed solution; placing the third mixed solution in a reactor, and subjecting the reactor to constant temperature treatment and then natural cooling to obtain a reactant; removing unreacted organic matter and inorganic ions in the reactant, drying the cleaned reactant to obtain a black powder; and subjecting the black powder to annealing and reduction treatment to obtain a ruthenium / molybdenum dioxide composite material. The electrode prepared from the raw materials RuCl3·xH2O and MoCl5 of the invention has the best water electrolysis hydrogen production performance; and the preparation process is simple, the steps are few, the time consumption is short, the cost is low, the composition is uniform, the morphology is controllable, and the like.
Owner:CHANGZHOU INST OF TECH

Electric pulse assisted forging process and tooling for two-phase / near-α titanium alloy

The present invention discloses a two-phase / near-α titanium alloy electric pulse assisted reforging process and tooling, and forging a two-phase / near-α titanium alloy ingot with a coarse Widmanstatten structure (cluster domain structure composed of lamellar structure) after opening by conventional large forging ratio forging composite high-frequency short-time high-temperature electrical treatment and low-frequency long-time low-temperature electrical treatment in the low temperature section of the two-phase region to obtain a fine-grained dual-state structure titanium alloy forging billet with a high degree of spheroidization of the primary α phase and precipitation of fine lamellar α phase in the transformed β phase matrix. The two-phase / near-α titanium alloy electric pulse assisted reforging process and tooling of the present invention significantly improve the single-pass deformation spheroidization rate of the titanium alloy, reduce the reforging heating temperature and the number of fires, reduce the production cost, simplify the process flow, thereby shortening the production cycle, improving the material utilization rate, and further improving the quality of the forging billet.
Owner:HEFEI UNIV OF TECH

Radiation cooling porous ceramic material and preparation method thereof

The invention discloses a radiation cooling porous ceramic material and a preparation method thereof. The ceramic material is prepared from the following components in percentage by mass: 0 to 30 weight percent of boron nitride nanosheets, 65 to 100 weight percent of silicon nitride and 0 to 5 weight percent of residual silicon. The practical application of building cooling can be met under the combined action of multiple scattering of sunlight by Si3N4, BN and porous ceramic pores and vibration absorption of a B-N bond of BN and an Si-N bond of Si3N4 in a wave band of 8-13 microns, and the building cooling material has a wide application prospect. A combustion synthesis method which is simple in process, efficient, low in cost and nearly zero in energy consumption is adopted, low-cost Si powder and B2O3 powder are used as raw materials, alpha-Si3N4 is used as a diluent, rare earth oxide is used as a sintering aid, and chemical furnace powder (mixed powder of Si and beta-Si3N4) is used for in-situ synthesis of the Si3N4-BN radiation cooling porous ceramic material in a nitrogen atmosphere.
Owner:苏州仿生材料科学与工程中心

Method for preparing quinine anion exchange membrane through pre-irradiation grafting

ActiveCN119455697Ahigh mechanical flexibilitygood chemical stabilityMembranesCellsElectrical batteryCarbon chain
The invention belongs to the technical field of anion exchange membranes, and discloses a method for preparing a quinine anion exchange membrane by pre-irradiation grafting, which comprises the following steps: by taking a linear carbon chain polymer as a base material and a vinyl monomer as a grafting monomer, firstly, pre-irradiating the linear carbon chain polymer by an electron beam radiation source; then carrying out a grafting reaction on the pre-irradiated base material in a vinyl monomer solution, and then carrying out a quaternary ammonification reaction on the obtained grafted polymer and a quinuclidine solution, so as to obtain the quinine anion exchange membrane. The method is relatively simple in overall process, mild in reaction condition, relatively short in reaction time and relatively low in cost; compared with a biphenyl quinine anion exchange membrane, the prepared quinine anion exchange membrane has higher anion conductivity and better mechanical strength, and compared with a piperidine anion exchange membrane and a trimethylamine anion exchange membrane, the prepared quinine anion exchange membrane has higher chemical stability; and the material can be applied to the fields of fuel cells, electrolyzed water, flow cells and the like.
Owner:TIANJIN UNIV

Construction method of pebble geological residence foundation sump caisson

The invention relates to a caisson construction method for a pebble geological residence foundation sump, which comprises the following steps of: arranging wall protection steel pipes at the periphery in a foundation pit, hammering the wall protection steel pipes to be downwards inserted into a pebble bed, and stacking sandbags on the outer sides of the wall protection steel pipes to form a sandbag protection slope to prevent the foundation pit slope from collapsing and prevent fine sand from rushing into the foundation pit. Concrete is poured on the inner side of the retaining wall steel pipe through an underwater concrete self-sinking method, the caisson foundation is formed, the caisson foundation can prevent water in the lower pebble bed from flowing upwards during water pumping, and the flatness of the caisson bottom foundation is guaranteed. According to the construction method, only the water level needs to be lowered to 500 mm below the foundation raft, it can be effectively prevented that when surface water flows into the sump through the pebble bed, a foundation pit slope collapses, a foundation base needs to be cleaned many times, the foundation trench size is increased again, compared with a traditional construction method, the cost is low, the construction period is short, and the construction quality is easy to guarantee. The method is high in practicability, the labor cost and the material cost of a using unit can be reduced, and control over the construction cost, the construction period and the quality of the sump is facilitated.
Owner:CHINA MCC20 GRP CORP LTD +1

Preparation method of MgAlON ceramic with high infrared transmittance

The invention relates to a preparation method of MgAlON ceramic with high infrared transmittance, and belongs to the field of ceramic preparation. The invention relates to a method for preparing MgAlON ceramic with high infrared transmittance, which comprises the following steps of: ball-milling and mixing 86.2-86.6 wt.% of gamma-Al2O3, 8.6 wt.% of MgO and 4.8-5.2 wt.% of carbon black, and synthesizing pure-phase MgAlON powder with an O / N value of 9.5-12.0 by adopting a carbon thermal reduction nitridation method; caCO3 is added into the pure-phase MgAlON powder to serve as a sintering aid, MgAlON / CaCO3 mixed powder is obtained after ball milling, and a green body is prepared through dry pressing; and preparing the MgAlON ceramic with high infrared transmittance by adopting a pressureless sintering method. According to the method, the MgAlON transparent ceramic is prepared by designing the composition of the raw material powder, regulating and controlling the nitridation degree of the MgAlON powder and further adopting the powder as the raw material through a pressureless sintering method under the condition of short heat preservation time, the raw materials are easy to obtain and low in price, the prepared MgAlON powder is good in sintering performance, and the MgAlON transparent ceramic is high in infrared transmittance, good in energy-saving effect, high in efficiency, low in cost and suitable for industrial production. And the process is simple and feasible, and is suitable for industrial production.
Owner:DALIAN MARITIME UNIVERSITY

Foaming forming mold and foaming part production equipment

ActiveCN119408044Aquick costlow costGlass fiberThermal water
The invention provides a foaming forming mold and foaming part production equipment, and relates to the technical field of foaming part production equipment, and the foaming forming mold comprises a concrete structure, a resin structure layer and a glass fiber reinforced plastic structure layer. The glass fiber reinforced plastic structure layer is arranged on the concrete structure; the resin structure layer is arranged on the glass fiber reinforced plastic structure layer, and aluminum profiles are embedded in the resin structure layer and the glass fiber reinforced plastic structure layer. The flow guide holes are formed in the aluminum profile and can guide hot water to pass through so as to heat the mold. A plurality of aluminum profiles are sequentially arranged at intervals in the extending direction of the contour line formed on the section along the forming surface, heat dissipation structures and flow guide holes are arranged on the aluminum profiles, the heat dissipation structures are in a long strip shape, and the extending direction of the heat dissipation structures is consistent with the extending direction of the contour line formed on the section along the forming surface. By means of the arrangement of the aluminum profiles, heat can be more evenly transmitted to the forming surface, and the foaming forming quality can be improved.
Owner:HU NAN YA TAI SHI YE YOU XIAN GONG SI

A colorimetric system based on precious metals and their alloys and its application

The present invention discloses a colorimetric system based on precious metals and their alloys, and application of the system in blood glucose detection. Compared with biochemical, electrochemical and currently available colorimetric methods, the colorimetric system based on precious metals and their alloys described in the present invention has the advantages of low cost, fast reaction speed, simple components, low sample consumption, high sensitivity, good selectivity, convenience and speed, and can realize accurate detection of glucose in complex actual body fluid samples, and has excellent clinical application potential.
Owner:SHANGHAI JIAOTONG UNIV

Combustion synthesis method of multi-component transition metal boride ceramic powder

The invention discloses a combustion synthesis method of multi-component transition metal boride ceramic powder, relates to the field of ultrahigh-temperature ceramic materials, and aims to solve the problems of low purity and low production efficiency of the index multi-component transition metal boride ceramic powder in the conventional method. The method comprises the following steps: 1, preparing transition metal oxide solid solution powder from transition metal powder and transition metal oxide powder through combustion synthesis; 2, adding boron oxide and magnesium powder into the transition metal oxide solid solution powder, and uniformly mixing; 3, putting the mixed material into a closed reaction container, igniting, and carrying out a self-propagating magnesiothermic reduction reaction; and 4, cooling, taking out a reaction product, and carrying out acid pickling, suction filtration, washing and drying to obtain the multi-component transition metal boride ceramic powder. According to the method, the production time is greatly shortened, and the production efficiency can be improved. The prepared multi-component transition metal boride ceramic powder has high purity and high solid solution degree. The method is used for preparing the multi-component transition metal boride ceramic powder.
Owner:HARBIN INST OF TECH

A method for creating diploid seedless watermelon by gene editing ClSPO11-2

The present invention discloses a method for creating diploid seedless watermelon by gene editing ClSPO11‑2, and belongs to the technical field of plant genetic engineering breeding. The present invention discloses a method for creating diploid seedless watermelon by gene editing ClSPO11‑2, and for the first time uses CRISPR / Cas9 technology to perform targeted editing on the watermelon ClSPO11‑2 gene, so that meiosis cannot be paired and synaptic, and male and female gametes are sterile, so that the gene function is lost, and the seeds are aborted after double fertilization, thereby creating seedless watermelon at the diploid level. Compared with wild-type materials, the edited diploid seedless watermelon plants created by this method have no obvious changes in phenotype during the vegetative growth stage, except for male and female gamete sterility, and the seedless phenotype can be infinitely propagated by plants edited by Clspo11‑2 / ClSPO11‑2 heterozygous. The method has the advantages of being simple and easy to operate, high efficiency, low cost and short cycle.
Owner:NORTHWEST A & F UNIV