Vaccine composition for preventing and treating respiratory diseases secondary to atrophic rhinitis, and preparation method and application thereof

A technology for respiratory diseases and vaccine compositions, which is applied in the field of vaccine compositions for respiratory diseases, and can solve problems such as difficulty in achieving immune effects, high cost, and short protection period

Active Publication Date: 2014-09-24
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of these vaccines has played a certain preventive role, but the immune effect of the vaccine is not ideal and the protection period is short. At the same time, the attenuated vaccine is not easy to store and there is a risk of loose virus.
[0007] The immunization program for porcine infectious pleuropneumonia vaccine is the first immunization for piglets at the age of 35 to 40 days, and a booster immunization once 4 weeks after the first immunization. 4ml within a single immunization; and the immunization procedure of the inactivated porcine atrophic rhinitis vaccine is the first immunization at the age of 7 to 10 days, and a booster immunization 2 weeks after the first immunization. Commercially available commercial vaccines need multiple immunizations, and the cost Higher, greater stress on pigs; with the prevalence of pathogenic strains and mixed infections, it is difficult for existing vaccines to achieve the expected immune effect. Vaccines for porcine atrophic rhinitis and porcine atrophic rhinitis are still blank at home and abroad
Moreover, when the inventor reproduced the non-progressive porcine atrophic rhinitis test of a pig secondary infection of porcine infectious pleuropneumonia, it was found that there was no Bordetella bronchiseptica pathogen detected, but there was still occasional mild Symptoms of non-progressive porcine atrophic rhinitis; when exogenous toxin-producing Pasteurella multocida capsular type A or D pathogens invade, it often causes severe secondary infection mixed with progressive porcine infectious pleuropneumonia Atrophic rhinitis is a comprehensive respiratory disease, but the inventor still did not detect the Bordetella bronchiseptica pathogen when testing

Method used

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  • Vaccine composition for preventing and treating respiratory diseases secondary to atrophic rhinitis, and preparation method and application thereof
  • Vaccine composition for preventing and treating respiratory diseases secondary to atrophic rhinitis, and preparation method and application thereof
  • Vaccine composition for preventing and treating respiratory diseases secondary to atrophic rhinitis, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Implementation 1 to prevent porcine infectious pleuropneumonia, porcine pasteurellosis and porcine atrophic rhinitis inactivated vaccine preparation method:

[0039] 1. Bacteria

[0040] Actinobacillus pleuropneumoniae type 1 LC strain, accession number: CCTCC M 2011458.

[0041] Actinobacillus pleuropneumoniae type 5 YC strain, accession number: CCTCC M 2011459.

[0042] Actinobacillus pleuropneumoniae type YS strain, accession number: CCTCC M 2011460.

[0043] Pasteurella multocida type A HN5 strain, deposit number: CCTCC M 2011222.

[0044] Pasteurella multocida D-type HB4 strain, deposit number: CCTCC M 2011221.

[0045] Pasteurella multocida type B is the capsule type B (group) Pasteurella multocida C44-1 strain, which is identified, kept and supplied by the China Veterinary Drug Administration.

[0046] Bordetella bronchiseptica strain HN8, deposit number: CCTCC M 2011223.

[0047] 2. Preparation of vaccines

[0048] 2.1 Preparation of seeds for production ...

Embodiment 2

[0100] Example 2 Comparison of the immunization effects of a single vaccine of Actinobacillus pleuropneumoniae, a single vaccine of Pasteurella suis, a single vaccine of Bordetella and the combined vaccine of the present invention

[0101] A total of 210 healthy and susceptible 3-week-old weaned piglets were selected and divided into two groups, 105 in the immunization group and 105 in the control group.

[0102] Among them, 35 piglets in the immunization group were immunized with the inactivated vaccine B of the present invention for porcine infectious pleuropneumonia, pasteurellosis and porcine atrophic rhinitis. The 3-week-old piglets were immunized at an interval of 3 weeks. Each virulent strain was used for challenge, and the method and dose of challenge were shown in 3.3 Efficacy Test in Example 1.

[0103] The other 30 animals in the immunization group were immunized with a single vaccine of Actinobacillus pleuropneumoniae (the antigen content was the number of live bac...

Embodiment 3

[0156] Example 3 Infection model of respiratory disease secondary to atrophic rhinitis

[0157] 6-week-old piglets were selected and divided into 8 groups. All piglets were inoculated with each bacterial venom via the oronasal route according to the protocol in Table 17

[0158] Table 17 Grouping table of infection models for respiratory diseases secondary to atrophic rhinitis

[0159]

[0160]

[0161] Test attack results:

[0162] Group A: The pigs were normal, without any symptoms, and the organs were normal in anatomy.

[0163] Group B: The pigs were normal and did not have any symptoms. The autopsy found that the turbinate bones were slightly atrophied, and there were no lesions in the lungs. No Bordetella was detected in the nasal swab.

[0164] Group C: The piglets had chronic symptoms, with occasional cough symptoms. The autopsy found that there were multiple necrotic foci in the lungs, and cellulose flocs were attached to the pleura and pericardium. The patho...

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Abstract

The invention provides a vaccine composition for preventing and treating respiratory diseases secondary to atrophic rhinitis, which comprises: an immunization amount of antigens of pig actinobacillus pleuropneumoniae; an immunization amount of antigens of pasteurella multocida; an immunization amount of antigens of brodetella bronchiseptica; and adjuvants. The vaccine composition has the advantages of high safety performance, good immunogenicity, long immunity period, low cost, and the like, can reduce cost, decrease immunization labor amount, and reduce animal stress.

Description

technical field [0001] The present invention relates to a vaccine composition for preventing and treating respiratory diseases secondary to atrophic rhinitis. Background technique [0002] Porcine infectious pleuropneumonia, porcine pasteurellosis and porcine atrophic rhinitis are common porcine respiratory diseases. Porcine pleuropneumonia is a highly lethal respiratory infectious disease in pigs caused by Actinobacillus pleuropneumoniae (APP), which occurs in countries all over the world. The disease mainly causes hemorrhagic and necrotizing pneumonia accompanied by pleurisy in pigs, mostly in the most acute or acute course and quickly kills. The disease can occur in pigs of any age, especially in intensive pig farms, and once it occurs, it can cause significant economic losses. [0003] Swine pasteurellosis is an infectious disease caused by Pasteurella multocida. The most acute form of the disease is characterized by septic changes, acute swelling of the throat, and h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/102A61K39/02A61K39/116A61P31/04A61P11/00
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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