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Preparation method of triple inactivated vaccine for pigs

An inactivated vaccine and inactivated technology, which is applied in the field of preparation of triple inactivated vaccines for pigs, can solve the problems of lack of vaccine preparation methods for Actinobacillus, reduce the risk of adverse product reactions, reduce immune side effects, and ensure immunity original effect

Inactive Publication Date: 2015-10-28
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the technical defects of the prior art, and provides a preparation method of a triple inactivated vaccine for pigs, so as to solve the lack of a simultaneous immunity to Haemophilus parasuis, Streptococcus suis and porcine infectious pleuropneumonia in the prior art. Technical Problems of Actinobacillus Vaccine Preparation Method

Method used

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  • Preparation method of triple inactivated vaccine for pigs
  • Preparation method of triple inactivated vaccine for pigs
  • Preparation method of triple inactivated vaccine for pigs

Examples

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Effect test

Embodiment 1

[0033] 1. Vaccine preparation

[0034] A preparation method of Haemophilus parasuis, Streptococcus suis, and porcine infectious pleuropneumonia triple inactivated vaccine, the steps are as follows:

[0035] 1 Production strain preparation

[0036] 1.1 First-class seed propagation

[0037] Haemophilus parasuis type 4, Haemophilus parasuis type 5, Streptococcus suis type 2, Actinobacillus pleuropneumoniae type 3, Actinobacillus pleuropneumoniae type 7, Porcine infectious pleuropneumoniae Freeze-dried strains of Actinobacillus type 10 were streaked and inoculated on TSA / NAD plates, cultured at 37°C for 18-24 hours, picked a single colony and inoculated in TSB / NAD liquid medium, and cultured at 37°C for 12-16 hours , is the primary seed.

[0038] 1.2 Secondary seed propagation

[0039] The prepared Haemophilus parasuis type 4, Haemophilus parasuis type 5, Streptococcus suis type 2, Actinobacillus pleuropneumoniae type 3, Actinobacillus pleuropneumoniae type 7, porcine infectio...

Embodiment 2

[0089] A preparation method for pig triple inactivated vaccine, comprising the following steps:

[0090] 1) Take the culture solution of Actinobacillus pleuropneumoniae type 3, the culture solution of Actinobacillus pleuropneumoniae type 7, and the culture solution of Actinobacillus pleuropneumoniae type 10 respectively, and separate the solid and liquid to collect the supernatant ;

[0091] 2) Concentrate the supernatants described in step 1) through ultrafiltration tubes, respectively add saturated ammonium sulfate to obtain precipitates, inactivate them respectively, concentrate them respectively, and adjust the toxin concentration to 200ug / mL respectively, thereby obtaining Apx Ⅰ antigen solutions respectively , Apx Ⅱ antigen solution and Apx Ⅲ antigen solution;

[0092] 3) Take Haemophilus parasuis type 4 culture solution, Haemophilus parasuis type 5 culture solution, and Streptococcus suis type 2 culture solution respectively, inactivate and concentrate respectively, an...

Embodiment 3

[0104] A preparation method for pig triple inactivated vaccine, comprising the following steps:

[0105] 1) Take the culture solution of Actinobacillus pleuropneumoniae type 3, the culture solution of Actinobacillus pleuropneumoniae type 7, and the culture solution of Actinobacillus pleuropneumoniae type 10 respectively, and separate the solid and liquid to collect the supernatant ;

[0106] 2) Concentrate the supernatants described in step 1) through ultrafiltration tubes, respectively add saturated ammonium sulfate to obtain precipitates, inactivate and concentrate respectively, and then adjust the toxin concentration to 400ug / mL, thereby obtaining Apx Ⅰ antigen solutions respectively , Apx Ⅱ antigen solution and Apx Ⅲ antigen solution;

[0107] 3) Take Haemophilus parasuis type 4 culture solution, Haemophilus parasuis type 5 culture solution, and Streptococcus suis type 2 culture solution respectively, inactivate and concentrate respectively, and then adjust the cell conce...

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Abstract

The invention provides a preparation method of a triple inactivated vaccine for pigs. The method determines an antigen composition with excellent immunization effects by selection of the antigen. The prepared polyvalent vaccine has outstanding immunization effects. The prepared vaccine contains a PCP immunization protective antigen exotoxin (Aps), has cross immunization protection effects better than those of a whole cell inactivated vaccine, greatly reduces side reaction, and can simultaneously prevent haemophilus parasuis, swine streptococcosis and actinobacillus pleuropneumonia by combined immunization with inactivated haemophilus parasuis and streptococcus suis. Compared with haemophilus parasuis and streptococcus suis inactivated vaccines sold on the market, the triple inactivated vaccine has the same corresponding pathogen immune protection force. Compared with the actinobacillus pleuropneumonia inactivated vaccine sold on the market, the triple inactivated vaccine has a cross immunization protecting force on diseased pigs with different serotypes and realizes multiple protection purposes.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to a method for preparing a triple inactivated vaccine for pigs. Background technique [0002] Haemophilus parasuis, Streptococcus suis and Actinobacillus pleuropneumoniae are important bacterial pathogens that endanger the pig industry today, often associated with porcine circovirus, porcine reproductive and respiratory syndrome virus, swine fever virus or The mixed infection of swine influenza virus seriously endangers the development of pig industry. [0003] Haemophilus parasuis (Haemophilus parasuis, HPs) is the pathogen of porcine Glasser's disease, which belongs to the genus Haemophilus of Pasteurellaceae, and is a commensal bacterium in the upper respiratory tract of pigs. A systemic disease characterized by inflammation, arthritis, and meningitis. HPs only infects pigs and has strong host specificity. Adult pigs do not show obvious symptoms. It most...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/116A61K39/102A61K39/09A61K39/02A61P31/04
Inventor 厚华艳吕茂杰杨保收付旭彬梁武
Owner TIANJIN RINGPU BIO TECH
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