Vaccine composition resisting porcine circovirus and porcine infectious pleuropneumonia infection and preparation

A technology of porcine circovirus and porcine pleuropneumonia, applied in antiviral agents, antibacterial drugs, antibody medical components, etc., can solve the problems of immune stress response and immune effect reduction

Active Publication Date: 2014-03-26
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] Therefore, the problem that exists at present is that it is necessary to research and develop a kind of immunization cost is low, adopts the adjuvant that livestock is easy to accept, can prevent porcine contagious pleuropneumonia, porcine circovirus type 2 infectious diseases at the same time, and can avoid multiple inoculations Vaccine causing immune stress response and immune effect reduction and preparation method thereof

Method used

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  • Vaccine composition resisting porcine circovirus and porcine infectious pleuropneumonia infection and preparation
  • Vaccine composition resisting porcine circovirus and porcine infectious pleuropneumonia infection and preparation
  • Vaccine composition resisting porcine circovirus and porcine infectious pleuropneumonia infection and preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1: Preparation and inspection of anti-porcine circovirus type 2 and porcine infectious pleuropneumonia infection vaccine composition

[0068] 1. Preparation of bacteria (virus) species for production

[0069] The preparation of porcine circovirus type 2 SH strain: virus seeds are used MEM medium ( Invitrogen Company) was diluted 10 times, inoculated in PK15 (ATCC, deposit number CCL-33) cell culture according to 5% volume, adsorbed at 37°C for 30min, added D-glucosamine hydrochloride containing 4% calf serum and 2mmol / L MEM cell maintenance medium, cultivated at 37°C for 4 days, freeze-thawed 2-3 times, harvested the virus, and the virus titer was 10 6.5 TCID 50 / ml.

[0070] Preparation of Actinobacillus pleuropneumoniae strains: Streak inoculation of Actinobacillus pleuropneumoniae type 1 LC strain, type 5 YC strain and type 7 YS strain in TSA / NAD plates were cultured at 37°C for 24 hours, and more than 5 typical colonies were selected each. After pas...

Embodiment 2

[0126] Example 2: Comparison of PCV2 inactivated whole virus antigen vaccine to PCV2 challenge immune effect and PCV2 inactivated virus antigen and porcine Actinobacillus pleuropneumoniae inactivated antigen vaccine composition comparison of PCV2 challenge immune effect

[0127] Antigens prepared in Example 1 (i.e. K001 batch of antigens, see Table 2) were used to prepare porcine circovirus type 2 and Actinobacillus pleuropneumoniae (serum type 1+serum type 4+serum type 5) vaccine compositions (V1) respectively and (V2), porcine circovirus vaccine (V3) and (V4), the specific formula is as follows:

[0128] 1. The total volume of porcine circovirus type 2 and Actinobacillus pleuropneumoniae (serotype 1 + serotype 4 + serotype 5) vaccine composition (V1) is about 100ml:

[0129]

[0130] 2. Porcine circovirus type 2, Actinobacillus pleuropneumoniae (serotype 1 + serotype 4 + serotype 5) vaccine composition (V2) has a total volume of about 100ml:

[0131]

[0132] 3. The t...

Embodiment 3

[0151] Example 3: Comparison of the protective effects of porcine infectious pleuropneumonia inactivated antigen vaccine composition, PCV2 inactivated virus antigen, and porcine infectious pleuropneumonia inactivated antigen vaccine composition against Actinobacillus pleuropneumoniae

[0152] Using the antigen K001 batch prepared in Example 1, the vaccine composition (V1), porcine circovirus type 2, Actinobacillus pleuropneumoniae (serum type 1 + serum type 5 + serum type 7) vaccine composition (V1), Actinobacillus pleuropneumoniae inactivated vaccine (serotype 1 + serotype 5 + serotype 7) (V2), the specific formula is as follows.

[0153] (1) Porcine circovirus type 2, Actinobacillus pleuropneumoniae (serotype 1 + serotype 5 + serotype 7) vaccine composition (V1):

[0154]

[0155] (2) Actinobacillus pleuropneumoniae inactivated vaccine (serotype 1 + serotype 5 + serotype 7) inactivated vaccine (V2):

[0156]

[0157] Select 50 weaned piglets aged 35 to 40 days and div...

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Abstract

The invention relates to a bivalent vaccine composition resisting type-2 porcine circovirus and porcine infectious pleuropneumonia infection and a preparation method. The bivalent vaccine composition comprises a type-2 porcine circovirus antigen and an actinobacillus pleuropneumoniae antigen; the immune procedure is simple; the type-2 porcine circovirus related diseases and porcine infectious pleuropneumonia can be effectively prevented at the same time. Compared with a single vaccine, the bivalent vaccine composition does not generate an immune interference phenomenon while the immune effect is not reduced; moreover, the actinobacillus pleuropneumoniae can enhance the immune effect of the type-2 porcine circovirus antigen. The preparation process of the bivalent vaccine composition is simple, the immune cost is low, and the practicability is high.

Description

technical field [0001] The invention belongs to the technical field of animal husbandry biopharmaceuticals, and relates to a dual vaccine composition against porcine circovirus type 2 and porcine infectious pleuropneumonia infection and a preparation method thereof. Background technique [0002] Porcine infectious pleuropneumonia is an important respiratory infectious disease caused by Actinobacillus pleuropneumoniae (APP). This disease mainly causes a kind of fibrinous, hemorrhagic, and necrotizing pneumonia accompanied by pleurisy in pigs. Most of them are the most acute or acute course and die quickly. The morbidity and mortality are more than 50%. It can occur at any age. Pigs, especially in intensive pig farms, will cause significant economic losses once it occurs. [0003] Clinically, it is generally divided into the most acute, acute, subacute and chronic. The most acute type mostly occurs in weaned piglets. One or several pigs suddenly become ill. The body temperat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61P31/04A61P31/20
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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