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Anti-porcine circovirus and porcine infectious pleuropneumonia vaccine composition and preparation

A technology for porcine circovirus and porcine pleuropneumonia, which is applied in the directions of antiviral agents, antibacterial drugs, and antibody medical ingredients, which can solve the problems of immune stress response and reduced immune effect.

Active Publication Date: 2015-08-19
PULIKE BIOLOGICAL ENG INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] Therefore, the problem that exists at present is that it is necessary to research and develop a kind of immunization cost is low, adopts the adjuvant that livestock is easy to accept, can prevent porcine contagious pleuropneumonia, porcine circovirus type 2 infectious diseases at the same time, and can avoid multiple inoculations Vaccine causing immune stress response and immune effect reduction and preparation method thereof

Method used

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  • Anti-porcine circovirus and porcine infectious pleuropneumonia vaccine composition and preparation
  • Anti-porcine circovirus and porcine infectious pleuropneumonia vaccine composition and preparation
  • Anti-porcine circovirus and porcine infectious pleuropneumonia vaccine composition and preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1: Preparation and inspection of vaccine composition against porcine circovirus type 2 and porcine pleuropneumonia infection

[0068] 1. Preparation of bacteria (virus) species for production

[0069] Preparation of porcine circovirus type 2 SH strain: use MEM medium ( Invitrogen) make a 10-fold dilution, inoculate PK15 (ATCC, accession number CCL-33) cell culture at 5% volume, adsorb at 37°C for 30 min, add 4% calf serum and 2mmol / L D-glucosamine hydrochloride MEM cell maintenance solution, cultured at 37°C for 4 days, freeze-thaw 2-3 times, harvest the virus, the virus titer is 10 6.5 TCID 50 / ml.

[0070] Preparation of Actinobacillus pleuropneumoniae strains: Actinobacillus pleuropneumoniae type 1 LC strain, type 5 YC strain and type 7 YS strain were streaked into TSA / with 5% (v / v) newborn calf serum NAD plates were cultured at 37°C for 24 hours, and 5 or more typical colonies were selected. After the pure test passed, they were used as first-level seeds. Pick ...

Embodiment 2

[0126] Example 2: Comparison of the immune effect of PCV2 inactivated whole virus antigen vaccine against PCV2 challenge and the vaccine composition of PCV2 inactivated virus antigen and Actinobacillus pleuropneumoniae inactivated antigen against PCV2 challenge

[0127] Example 1 was used to prepare antigens (ie K001 batch of antigens, see Table 2) respectively to prepare porcine circovirus type 2 and Actinobacillus pleuropneumoniae (serotype 1 + serotype 4 + serotype 5) vaccine composition (V1) And (V2), porcine circovirus vaccine (V3) and (V4), the specific formula is as follows:

[0128] 1. Porcine circovirus type 2 and Actinobacillus pleuropneumoniae (serotype 1 + serotype 4 + serotype 5) vaccine composition (V1) total volume is about 100ml:

[0129]

[0130] 2. Porcine circovirus type 2, Actinobacillus pleuropneumoniae (serotype 1 + serotype 4 + serotype 5) vaccine composition (V2) total volume is about 100ml:

[0131]

[0132] 3. The total volume of porcine circovirus type 2 ina...

Embodiment 3

[0151] Example 3: Comparison of porcine infectious pleuropneumonia inactivated antigen vaccine composition and PCV2 inactivated virus antigen and porcine infectious pleuropneumonia inactivated antigen vaccine composition on the protection effect of Actinobacillus pleuropneumoniae challenge

[0152] Using the antigen K001 batch prepared in Example 1, the vaccine composition (V1) of porcine circovirus type 2 and Actinobacillus pleuropneumoniae (serotype 1 + serotype 5 + serotype 7) vaccine composition (V1), Swine Actinobacillus pleuropneumoniae inactivated vaccine (serotype 1 + serotype 5 + serotype 7) (V2), the specific formula is as follows.

[0153] (1) Porcine circovirus type 2, Actinobacillus pleuropneumoniae (serotype 1 + serotype 5 + serotype 7) vaccine composition (V1):

[0154]

[0155] (2) Inactivated vaccine against Actinobacillus pleuropneumoniae (serotype 1 + serotype 5 + serotype 7) inactivated vaccine (V2):

[0156]

[0157] Choose 50 weaned piglets from 35 to 40 days old...

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Abstract

The invention relates to a bivalent vaccine composition resisting type-2 porcine circovirus and porcine infectious pleuropneumonia infection and a preparation method. The bivalent vaccine composition comprises a type-2 porcine circovirus antigen and an actinobacillus pleuropneumoniae antigen; the immune procedure is simple; the type-2 porcine circovirus related diseases and porcine infectious pleuropneumonia can be effectively prevented at the same time. Compared with a single vaccine, the bivalent vaccine composition does not generate an immune interference phenomenon while the immune effect is not reduced; moreover, the actinobacillus pleuropneumoniae can enhance the immune effect of the type-2 porcine circovirus antigen. The preparation process of the bivalent vaccine composition is simple, the immune cost is low, and the practicability is high.

Description

Technical field [0001] The invention belongs to the technical field of animal husbandry biopharmaceuticals, and relates to a dual vaccine composition against porcine circovirus type 2 and porcine infectious pleuropneumonia infection and a preparation method thereof. Background technique [0002] Porcine infectious pleuropneumonia is an important infectious disease of the respiratory system caused by Actinobacillus pleuropneumoniae (APP). This disease mainly causes fibrinous, hemorrhagic, and necrotizing pneumonia in pigs with pleurisy, most of which are in the most acute or acute course and die quickly. The morbidity and mortality are above 50%. It can occur at any age Of pigs, especially in intensive pig farms, once it happens, it will cause major economic losses. [0003] Clinically, it is generally divided into the most acute, acute, subacute and chronic. The most acute type mostly occurs in weaned piglets. One or a few pigs have a sudden illness. The sick pig has a body tempe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61P31/04A61P31/20
Inventor 廖永洪李向东张许科孙进忠白朝勇
Owner PULIKE BIOLOGICAL ENG INC
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