40 results about "Pleuronectes pinnifasciatus" patented technology

The invention discloses a detection kit for Actinobacillus pleuropneumoniae and its application. The kit provided by the present invention includes three pairs of primers, namely an inner primer pair, an outer primer pair and a circular primer pair combined with the 3' end 1000bp gene in the Actinobacillus pleuropneumoniae Gen Bank Accession Number AF021919 sequence. The kit also includes a loop-mediated isothermal amplification reagent, a positive control, a negative control and a fluorescent chromogenic reagent, and the positive control is the DNA of Actinobacillus pleuropneumoniae. The invention also provides a method for detecting whether the animal carries the Actinobacillus pleuropneumoniae by using the kit of the invention. The kit of the present invention has high detection sensitivity, 6-10 copies can detect the target DNA, is simple and convenient to operate, and is especially suitable for the detection of pathogens carried out at the grassroots level and the detection of Actinobacillus pleuropneumoniae in possibly contaminated animal food .

The invention discloses a method for producing glutathione by using bifunctional glutathione synthetase contained in actinobacillus pleuropneumonia, actinobacillus succinogenes, bacillus cereus, streptococcus sanguis, streptococcus gordonii, streptococcus uberis and streptococcus thermophilus. The method comprises heterologous expression by using the seven microbes or enzymes of the microbes in other microbes. The glutathione synthesized by using the method has the structure of natural glutathione, and has high response rate and high output and yield of the glutathione.

The invention relates to a haemophilus parasuis detection kit and a detection method thereof, and belongs to the technical field of molecular biology. The detection kit comprises a primer pair, PCR Mix, a position control and dd H2O. The haemophilus parasuis detection kit disclosed by the invention has the primer pair designed according to an mviN gene sequence in a high conserved domain, is good in specificity, and can accurately distinguish the haemophilus parasuis strain LC from haemophilus paragallinarum, actinobacillus pleuropneumoniae, pasteurella muhocida, arcanobacterium pyogenes, staphylococcus aureus and streptococcus suis; the detection kit and the detection method provided by the invention are high in sensitivity, short in consumed time, accurate in detection, and important in significance of monitoring haemophilus parasuis reproduction, disease occurrence and prevalence as well as timely control of the disease.

The invention discloses a resistance marker-free attenuated live vaccine against porcine contagious pleuropneumonia (PCP) and application thereof and further discloses a resistance marker-free actinobacillus pleuropneumoniae (APP) serum 7 type three gene deletion strain used for preparing the vaccine, belonging to the field of bacterial gene engineering technology. The resistance marker-free APP serum 7 type three gene deletion strain APP-Delta clpP-Delta apxIIC-Delta fur is a strain obtained by inactivating coding genes of protease ClpP, an ApxII toxin activator ApxIIC and an iron absorption regulatory protein Fur of APP through oriented homologous recombination and destroys expression of ClpP, ApxIIC and the Fur protein. The three gene deletion strain obtained in the invention has smaller virulence, decreased by more than 100 times, compared with that of a parent strain, and is safe to animals; A pig immunized by using the strain is well protected from attacks by different serum type virulent strains of APP, and all the protection rates are greater than 80%; and the three gene deletion strain can be used as the attenuated live vaccine for immunoprophylaxis of PCP.

The invention relates to the field of veterinary drugs, in particulrl to a composition for treating swine mycoplasmal pneumonia and porcine contagious pleuropneumonia and a preparation method thereof. The composition is prepared from, by weight, 15-20 parts of rhizoma atractylodis, 15-20 parts of radix saposhnikoviae, 10-13 parts of liquorice, 10-15 parts of apricot kernels, 30-35 parts of radix astragali, 15-20 parts of radix asteris, 10-15 parts of flos farfarae, 10-15 parts of herba ephedrae, 10-15 parts of periostracum cicada, 10-15 parts of fructus forsythiae and 20-30 parts of lonicera japonica. The composition has the effects of clearing heat, discharging fire, diminishing inflammation, resisting bacteria and the like, all the traditional Chinese medicines are proportioned reasonably to complement and coordinate mutually, the good treatment effect on the swine mycoplasmal pneumonia and the porcine contagious pleuropneumonia is achieved, the treatment course is short, the effects are rapid to achieve, and the cure rate is high; meanwhile, the preparation method of the composition is simple, safe, reliable, free of pollution and residues and easy to popularize.

The invention relates to a vaccine composition against swine mycoplasma pneumonia and infectious pleuropneumonia and a preparation method thereof. The vaccine composition contains Mycoplasma hyopneumoniae antigen and Actinobacillus pleuropneumoniae antigen, has simple immunization procedures, can produce antibodies, and has protection against viruses at the same time, and can effectively prevent and treat Mycoplasma pneumoniae pneumonia and porcine infectious pleuropneumonia. The immune effect of the vaccine composition is at least equal to the effect of separate injection of single vaccine, less side effects, high serum antibody titer, long immunization period, less time-consuming, less labor, and less damage to pigs. The vaccine composition has simple production process, low immunization cost and strong practicability.

The invention discloses an actinobacillus pleuropneumoniae serotype 2 bacterial strain and its preparation method, which is actinobacillus pleuropneumoniae serotype 2XT, wherein the CCTCC NO. is M2011410. The preparation method comprises the following steps: 1) medium preparation, A) weighting yeast powder, glucose, monosodium glutamate, MgSO4, FeSO4.7H2O, dissolving into pure water, regulating pH value and disinfecting; B) weighting NaH2PO4 and K2HPO4 to prepare mother liquor and disinfecting; C) weighting NAD to prepare mother liquor, filtering by a filter membrane; D) mixing the solutions obtained in the step A, the step B and the step C according to amount to obtain the medium; 2) fermentation and culture, a) activating the freeze-drying seeds by NAD-contained TSA plates until single colony is grown out; b) selecting single colony and culturing by shaking a bottle; c) transferring cultured seed liquid to the fermentation medium; d) low stirring at initial fermentation period, and low ventilating; e) raising rotating speed at logarithmic phase and ventilating, and on-line controlling pH value; f) improving dissolved oxygen, and placing into a tank and collecting bacterium. The actinobacillus pleuropneumoniae serotype 2 bacterial strain suitable for preparing inactivated vaccine has the advantages of strong toxicity, good antigen effect, low price, fast mycelium growth, highdensity and easy control.

The invention relates to a preparation method of APP (Actinobacillus Pleuropneumoniae) OMVs (Outer Membrane Vesicles) and a vaccine of the APP OMVs, and belongs to the technical field of biology. The preparation method comprises the steps of culturing APP shope strains in vitro by using a culture medium which is in iron ion limit, obtaining acellular cultural supernatant after carrying out centrifugation and 0.22-[mu]m filtration treatment, and preparing the OMVs released by germs through ultracentrifugation, wherein the observation through a transmission electron microscope shows that the diameter of most OMVs is 50 to 100 nm, the OMVs are used as subunit vaccines to carry out secondary intranasal immunization on a mouse, the weight of the OMV immune mouse is increased for a long time, and the visual forms of lungs have no obvious difference from a PBS (Phosphate Buffer Saline) immune group. Meanwhile, an experiment shows that the OMVs are efficient immune stimulants, not only can high-level IgG (Immunoglobulin G) be stimulated to be generated by mouse sera, but also high-level IgA (Immunoglobulin A) can be generated in mouse lungs, mucosal immunity of the mouse lungs is effectively stimulated, and a better application prospect of using the OMVs as the subunit vaccines is expressed.