Resistance marker-free porcine actinobacillus pleuropneumoniae double-gene defective strain, construction method and application thereof

A technology for porcine pleuropneumoniae and Actinobacillus, which is applied in the field of bacterial genetic engineering, can solve the problems of inability to prevent pulmonary lesions and chronic infection, and inability to provide cross-protection for heterologous serotype infection, and achieves the effect of broad market application prospects.
CN102517232AInactive Publication Date: 2012-06-27HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Publication Date
2012-06-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a resistance marker-free porcine actinobacillus pleuropneumoniae (APP) serum type 7 double-gene defective strain, and belongs to the technical field of bacterial gene engineering. The recombinant strain APPdeltaclpPdeltaapx II C of APP is obtained by inactivating ClpP protease in the APP and a coded gene of a hemolysin activated factor Apx II C by adopting a directional homologous recombination technology, and expression of the ClpP protease and the Apx II C protein is destroyed. The obtained double-gene defective strain has lower toxicity compared with a parent strain, is safe to animals, provides an important basis for transformation of porcine contagious pleuropneumonia (PCP) vaccines and research of matched identification and diagnosis reagents, and has great significance for promoting elimination and purification of the global PCP.
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Description

technical field

[0001] The invention relates to a double gene deletion strain of Actinobacillus pleuropneumoniae ClpP and ApxIIC without resistance markers, a construction method and application thereof, and belongs to the technical field of bacterial genetic engineering. Background technique

[0002] Porcine contagious pleuropneumonia (PCP) is a highly contagious and lethal respiratory infectious disease caused by Actinobacillus pleuropneumoniae (APP). At present, the disease has been widely prevalent in countries all over the world, causing huge economic losses, and has become one of the important infectious diseases that is internationally recognized as an important threat to the modern pig industry. With the large-scale and intensive development of my country's modern breeding industry, the occurrence of this disease is showing an outbreak trend, and the positive rate of some pig farms has reached more than 70%, which has seriously hindered the healthy development of my ...

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