Porcine contagious pleuropneumonia trivalent inactivated vaccine and its preparation method

A technology of porcine pleuropneumonia and pleuropneumonia, applied in the field of veterinary drug vaccines, can solve the problems of fever and easy residue formation, and achieve the effects of small immune stress, good safety, and avoiding oil residue problems

Active Publication Date: 2013-07-24
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mineral oil cannot be metabolized, so it will produce a series of side effects, including ulcers, granulomas, fever, etc., and it is easy to form residues

Method used

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  • Porcine contagious pleuropneumonia trivalent inactivated vaccine and its preparation method
  • Porcine contagious pleuropneumonia trivalent inactivated vaccine and its preparation method
  • Porcine contagious pleuropneumonia trivalent inactivated vaccine and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The identification of the isolation of embodiment 1 serotype 1 LC strain, 5 type YC strain, 7 type YS strain

[0037] 1 Materials and methods

[0038] 1.1 The disease materials to be tested were collected from 10 pig farms in Henan, Hubei and other provinces and cities with suspected pleuropneumonia.

[0039] 1.2PCR primer sequence

[0040] PF-5'CCGACTTTTAAATCCGT3', PR-5'GAACAGTTGTTCGCTAA3'

[0041] 1.3 Mice were purchased from Animal Experiment Center of Henan Province.

[0042] 1.4 Isolation and purification of pathogenic bacteria and liquid culture Aseptically take lungs, throat tonsils and other tissues of dying pigs, inoculate them on TSA (containing 1% NAD) agar medium, place 10% CO2, culture at 37°C for 24-36 hours, and then select typical A single colony was cultured for purification. The purified single colonies were selected and inoculated in TSB (containing 1% NAD) liquid culture medium, and cultured overnight on a shaker (180 r / min) at 37°C.

[0043] 1....

Embodiment 2

[0060] Example 2: Preparation and efficacy test of porcine pleuropneumonia trivalent inactivated vaccine

[0061] 1 Materials and methods

[0062] 1.1 Culture medium: Actinobacillus pleuropneumoniae culture medium was cultured with Tryptic Soy Broth (TSB) and Tryptic Soy Agar Medium (TSA), purchased from BD Difco Company, batch number 0169092. The preparation process was carried out according to the instructions, and all the prepared TSA and TSB medium contained 1% NAD.

[0063] 1.2 Preparation of production seeds

[0064] Take out 1 bottle each of the basic seeds of Actinobacillus pleuropneumoniae serum type 1, 5, and 7, inoculate them on TSA medium by streaking, culture at 37°C for 24 hours, select 6-8 typical colonies and mix them in TSB broth, Inoculate the TSA slant, incubate at 37°C for 24 hours, and store in a refrigerator at 4°C, according to Appendix 15 of "The Veterinary Pharmacopoeia of the People's Republic of China" (2005 Edition). Those that pass the inspectio...

Embodiment 3

[0090] Example 3 Immune Effect and Comparative Test of Porcine Infectious Pleuropneumonia Trivalent Inactivated Vaccine

[0091] 1 Materials and methods

[0092] 1.1 Vaccines

[0093] Three batches of porcine infectious pleuropneumonia trivalent inactivated vaccines prepared according to Example 2, batch numbers: 200701, 200702, 200703, self-made oil emulsion inactivated vaccines as control vaccines, containing 1, 5, 7 strains LC, YC, YS strain.

[0094] Preparation of oil emulsion inactivated vaccine: prepare type 1, 5, and 7 strain antigens according to the antigen preparation method in Example 2, and mix 3 kinds of antigens after passing the inspection. After mixing, the content of each antigen is 5 × 10 9 CFU / ml, stored at 4°C for future use. Add 4% Tween-80 to the water phase (antigen), mix the water phase and the oil phase at a ratio of 2:3, and emulsify with a homogenizer. , Dispense the emulsified vaccine into sterilized vaccine bottles as the control vaccine. The...

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Abstract

Three porcine contagious Actinobacillus pleuropneumoniae comprising a serotype 1 LC strain, a serotype 5 YC strain and a serotype 7 YS strain are obtained in the invention; and a porcine contagious pleuropneumonia trivalent inactivated vaccine and its preparation method are obtained through the reasonable preparation of inactivated antigens of the above three strains to effectively prevent and control the propagation and the burst of porcine contagious pleuropneumonia in a culturing farm. The invention also provides a porcine contagious pleuropneumonia trivalent inactivated vaccine using an aqueous adjuvant. The porcine contagious pleuropneumonia trivalent inactivated vaccine furthest reduces the stress response during injection, and continuously improves the immune protection effect, so the porcine contagious pleuropneumonia trivalent inactivated vaccine is especially suitable for the large-scale application in Chinese culturing farms.

Description

technical field [0001] The invention relates to an inactivated porcine infectious pleuropneumonia vaccine, in particular to a polyvalent inactivated porcine infectious pleuropneumonia vaccine, which belongs to the field of veterinary drug vaccines. Background technique [0002] Porcine contagious pleuropneumonia (PCP) is a porcine contagious respiratory disease caused by Actinobacillus pleuropneumoniae (APP). The disease occurs in pigs of all ages, and the morbidity and mortality are above 20%. It is characterized by lung injury, hemorrhagic necrosis, and fibrosis. Clinical features can be seen in acute and chronic infections. Chronic infection can lead to decreased daily gain, slow growth, and low feed utilization. The disease was first reported by Pattiosn et al. in 1957, and has gradually spread since then, and has now spread all over the world. Serious economic losses have been caused to the pig industry in the world. [0003] The comprehensive prevention and control ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/116A61K39/02A61K39/39A61P31/04C12N1/20C12R1/04
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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