45results about How to "Reduce immune stress" patented technology

The invention discloses freshwater fish feed and a preparation method thereof. The feed comprises the following materials in percentage by weight: 5%-17% of import fish powder, 8%-22% of flour, 3%-16% of potato starch, 3%-18% of beer yeast, 5%-15% of bean pulps, 5%-15% of peanut pulps, 5%-10% of rape seed cakes, 2%-8% of corn powder, 3%-9% of cooked egg protein, 5%-8% of dried meat floss powder, 1%-2% of liquid phytase, 1%-3% of non-starch polysaccharase, 0.5%-1.9% of glutamine, 1%-2% of microecological preparation and 1%-3% of immune polysaccharides. The preparation process comprises a crushing step, a mixing step, a pelletizing step and a spraying step. The freshwater fish feed has the beneficial effects of being capable of well satisfying the nutrition needs of freshwater fish, improving the immunity of the freshwater fish, prompting intestinal absorption and improving the production performance of the freshwater fish.

The invention belongs to the technical field of feed for laying hens and particularly relates to complete feed for laying hens at brood time and a preparation method thereof. The complete feed comprises, by weight, 37-44% of corn, 18-22% of extruded corn, 0.4-0.6% of soybean oil, 18-20% of soybean meal, 9-11% of extruded soybeans, 1.5-2.5% of fish meal, 3.5-4.5% of fermented soybean meal, 1.1-1.2% of mountain flour, 1.4-1.7% of calcium hydrophosphate, 0.14-0.16% of methionine, 0.09-0.13% of choline chloride, 0.1-0.2% of baking soda, 0.2-0.3% of salt, 0.13-0.16% of microecologics, 0.01-0.03% of liquid phytase, 0.03-0.05% of liquid xylanase, 0.1-0.2% of acidifier, 0.1-0.2% of glycine betaine, 0.004-0.006% of antioxidant, 0.015-0.035% of 4% enramycin, 0.03-0.04% of vitamin complex and 0.08-0.09% of composite microelement. The complete feed can meet nutrition requirements of the laying hens at the brood time, improves immunity of the laying hens at the brood time, has remarkable effects on increasing weight and shin length of the laying hens at the brood time, enables the laying hens to reach the standard in advance, and provides a good foundation for achieving high yield at an egg producing period.

The invention discloses a preparation method of a swine vaccine specific swine spleen transfer factor (TF). The preparation method comprises the following steps: slaughtering a swine with a positive swine vaccine antibody to harvest the swine spleen; preserving the swine spleen at low temperature; unfreezing the swine spleen; removing fasciae; mincing the swine spleen; homogenizing the pulp; filling the homogenized pulp into a bottle and storing the pulp; repeatedly freezing and thawing the pulp; centrifuging the pulp; carrying out microfiltration; carrying out ultrafiltration; carrying out inactivation; regulating the pH value; regulating the osmotic pressure; removing bacteria; and detecting the quality. The preparation method has the advantages that the pH value of water for injection is regulated to 4-6 in the pulp homogenizing step, thus being beneficial to increasing of the yields of ribose and polypeptide; a box type membrane coating is adopted for tangential flow filtration, so that the dead volumes of system residues are small and linear amplification production is easy to achieve; a 1-3KD box type membrane coating is adopted to carry out tangential flow nanofiltration on a TF crude product, thus preparing the high-concentration TF and improving the using effects; phenol red is used as an acid-base indicator, thus being convenient for clients to observe the pH value of the TF; the TF uses beta-propiolactone for inactivation instead of traditional formaldehyde and an osmotic pressure regulation process is added, thus being beneficial to combined immunization of the TF and vaccines.

The invention provides a preparation method of a bivalent vaccine by taking newcastle disease virus La Sota strain and infectious bronchitis virus N-S multi-epitope protein as antigen. To immunize 21-day-old chickens, the bivalent vaccine prepared by the method disclosed by the invention can be used for simultaneously preventing newcastle disease and nephrotropic infectious bronchitis virus infection, and the antibody level of the bivalent vaccine is higher than that of respectively immunizing by single vaccine, so that immunological stress is effectively relieved and immunity time is reduced.

The invention provides compound feed with grape seed meal for 0 to 3-yearling laying hens and a preparation method thereof. The compound feed comprises the following raw materials: 56 to 61 percent of corn, 4 to 6 percent of flour, 25 to 27 percent of soybean meal, 3 to 6 percent of grape seeds, 0.4 to 06 percent of soybean oil, 1.2 to 1.5 percent of stone dust, 1.2 to 1.8 percent of calcium biphosphate, 0.2 to 0.4 percent of salt, 0.02 to 0.08 percent of sodium butyrate, 0.2 to 0.4 percent of acidulant, 0.05 to 0.15 percent of emulsifier, 0.02 to 0.05 percent of microecological preparation and 0.03 to 0.07 percent of choline chloride. All the raw materials are micron pulverized, then 100% of the pulverized materials pass through a 30-mesh standard sieve to be granulated under the temperature of 80 DEG C, and the gelatinization degree reaches 90 percent. The compound feed can satisfy the nutrition demand of brooding of laying hens, so a foundation can be set for the high productivity of the laying hens in the laying period.

The invention discloses a feed for piglets with reduced serum lipopolysaccharide level and its preparation method and application, which solves the problem that the disease resistance and immunity of piglets cannot be improved in the prior art. The invention is made of the following components according to a certain weight ratio: corn, puffed corn, broken rice, puffed soybean, fermented soybean meal, fish meal, soybean meal, soybean oil, stone powder, calcium hydrogen phosphate, sodium chloride, choline chloride, betaine , lysine, methionine, threonine, tryptophan, benzoic acid, phytase, compound enzyme, trace element premix, vitamin premix, enramycin premix, arginine, glutamine Amide, proline, astragalus polysaccharide, eleuthero polysaccharide, curcumin, chitosan oligosaccharide, probiotics, yeast cell wall extract. The composition of the invention is scientific, the compatibility is reasonable, each component acts together, can provide balanced and easily digestible nutrients for the growth and development of piglets, and effectively reduce the level of serum lipopolysaccharide of piglets.

The invention relates to the technical field of immunology and antibody preparation, and in particular to an anti-human COVID-19 antibody and a preparation method thereof. The antibody is obtained byimmunizing healthy poultry animals with COVID-19 inactivated vaccine, collecting water-soluble components in serum or yolk when the titer of the anti-COVID-19 antibody in the serum or yolk of the immunized poultry animals meets a requirement, and purifying the water-soluble components. Corresponding antibody dry powder can be obtained by carrying out spraying, freeze-drying and other treatments onan obtained product. The antibody obtained according to the preparation method can be used for treating patients in early, middle and later stages of COVID-19, can also be used for preventing the COVID-19, and can also be matched with COVID-19 vaccine for use to enhance the prevention and control effect of the COVID-19.

The present invention discloses a compound feed for laying hens in an egg production period. The compound feed consists of the main compositions in parts by weight: 100-120 parts of corn, 3-5 parts of corn pollen, 40-50 parts of soybean meal, 5-10 parts of wheat bran, 5-10 parts of peanut shell materials, 3-5 parts of carrot powder, 3-5 parts of fish steak powder, 3-5 parts of pine needle powder, 3-5 parts of fructus xanthii cake meal, 0.3-0.5 part of a macleaya cordata extract, 0.1-0.3 part of bacillus subtilis, 20-30 parts of shell powder, 5-8 parts of medical stones, 3-5 parts of calcium hydrogen phosphates, 3-5 parts of sodium chloride, 1-4 parts of baking soda, 5.8-7.9 parts of linoleic acids, 0.5-1 part of compound trace element, 0.5-1 part of compound vitamin and 0.3-0.5 part of a solid compound enzyme preparation. After the laying hens are fed with the compound feed, the egg production amount of the laying hens is large, the egg shell quality is good, the egg broken rate is low, the egg shapes are good, and the single egg weight is high.

The invention relates to an application of lavender essential oil as a feeding antibiotic substitute in daily ration of broiler, which belongs to the technical field of broiler raising, in particular to an application of lavender essential oil which can replace the feeding antibiotic in daily ration of broiler. By the invention, the lavender essential oil is added to the daily ration of broiler in a certain proportion, which not only can completely replace various feeding antibiotics in the formula of the daily ration of broiler, but also have the functions of ensuring the health of broiler, promoting the growth of broiler, and improving the transfer efficiency of feedstuff. The application can be used in production of broiler and products thereof without adding feeding antibiotics.

The invention discloses a preparation method of a swine vaccine specific cattle spleen transfer factor (TF). The preparation method comprises the following steps: slaughtering a cattle with a positive swine vaccine antibody to harvest the cattle spleen; preserving the cattle spleen at low temperature; unfreezing the cattle spleen; removing fasciae; mincing the cattle spleen; homogenizing the pulp; filling the homogenized pulp into a bottle and storing the pulp; repeatedly freezing and thawing the pulp; centrifuging the pulp; carrying out microfiltration; carrying out ultrafiltration; carrying out inactivation; regulating the pH value; regulating the osmotic pressure; removing bacteria; and detecting the quality. The preparation method has the advantages that the pH value of water for injection is regulated to 4-6 in the pulp homogenizing step, thus being beneficial to increasing of the yields of ribose and polypeptide; a box type membrane coating is adopted for tangential flow filtration, so that the dead volumes of system residues are small and linear amplification production is easy to achieve; a 1-3KD box type membrane coating is adopted to carry out tangential flow nanofiltration on a TF crude product, thus preparing the high-concentration TF and improving the using effects; phenol red is used as an acid-base indicator, thus being convenient for clients to observe the pH value of the TF; the TF uses beta-propiolactone for inactivation instead of traditional formaldehyde and an osmotic pressure regulation process is added, thus being beneficial to combined immunization of the TF and vaccines.

The invention aims to provide a novel duck reovirus compound vaccine and a preparation method of an egg yolk antibody. The preparation method comprises the following steps: mixing recombinant PVAX1-sigma B plasmid containing a novel duck reovirus sigma B protein gene and a novel duck reovirus sigma C protein according to a certain ratio, and performing emulsifying with a white oil adjuvant to prepare the compound vaccine. The average antibody titer of eggs collected 7-150 days after the three times of immunization reaches 1:1024 or above, and the highest antibody titer can reach 1:4096. The egg yolk antibody product prepared by extracting and purifying hyper-immune eggs can provide complete protection for ducks infected with the novel duck reovirus. The novel duck reovirus egg yolk antibody prepared by the method disclosed by the invention is definite in effect and low in cost, and has remarkable economic and social benefits.

The present invention discloses a preparation method for a poultry vaccine-specific pig spleen transfer factor. The preparation method comprises: selecting poultry vaccine antibody-positive pigs, and slaughtering to obtain pig spleen; carrying out cold preservation; thawing the pig spleen; removing fascia; mincing; homogenizing; filling into a bottle, and storing; carrying out repeated freezing and thawing; carrying out centrifugation; carrying out microfiltration; carrying out ultra-filtration; inactivating; adjusting the pH value; adjusting an osmotic pressure; sterilizing; and testing quality. According to the present invention, in the homogenizing step, the pH value of water for injection is adjusted to 4-6 so as to easily increase yields of ribose and polypeptides; the box type member package tangential flow filtration is adopted, such that the residual dead volume of the system is small, and linear enlargement production is easily achieved; the transfer factor crude product is subjected to tangential flow nano-filtration through a 1-3 KD box type member package to prepare the high concentration transfer factor so as to increase a use effect; phenol red is adopted as a pH value indicator, such that customers easily observe the pH value of the transfer factor; and beta-propiolactone is adopted to replace the traditional formaldehyde to inactivate the transfer factor, and the osmotic pressure adjustment process is added so as to easily achieve transfer factor and vaccine combined immunization.

The invention discloses a Chinese herbal medicine feed additive. The feed additive comprises the following components by weight: 63-70 of solid psidium guajava raw material, 2-4 of radix gentianae, 7-13 of radix isatidis, 3-7 of honeysuckle and 13-17 of vine of multiflower knotweed, wherein the solid psidium guajava raw material, radix gentianae, radix isatidis, honeysuckle and vine of multiflower knotweed are powder with grain size being 50-100 meshes. The feed additive has the following beneficial effects that the product belongs to the Chinese herbal medicine premix, is all natural and residue-free, promotes production and increases the capacity and the economic benefits; the product can improve the daily gain of poultry, reduce the feed conversion ratio, replace the antibiotic in the feed and play a role in preventing diseases; and the utilization ratios of the active ingredients of psidium guajava are increased from the original 30% to 80%.

The invention discloses a culture method for DHA-rich Metapenaeus ensis. The Metapenaeus ensis is fed with a feedstuff prepared from the following raw materials in parts by weight: 20-30 parts of peanut powder, 10-25 parts of bean pulp, 2-5 parts of earthworm powder, 0-10 parts of Schizochytrium limacinum powder, 10-25 parts of Schizochytrium limacinum waste residue, 1-5 parts of Bacillus natto, 0.2-0.5 part of yeast extract, 10-25 parts of maize germ oil and 1-5 parts of herba houttuyniae extracting solution. The culture method disclosed by the invention is low in cost, safe and reliable, and causes no pollution; the Metapenaeus ensis is fed with the feedstuff using the Schizochytrium limacinum and the waste residue thereof as the raw materials, thereby increasing the DHA and EPA contents in the prawn meat, enhancing the immunity of the Metapenaeus ensis, and promoting the growth and development of the Metapenaeus ensis. The culture method is applicable to large-scale culture of DHA-rich safe and functional Metapenaeus ensis products.

The invention discloses a fresh water fish forage. The fresh water fish forage is composed of, by weight, 8 to 16 parts of fish meal, 15 to 35 parts of soybean meal, 20 to 40 parts of peanut meal, 15to 25 parts of wheat, 15 to 30 parts of rapeseed cake, 5 to 10 parts of corn flour, 1 to 3 parts of liquid phytase, 1 to 2 parts of lactic acid bacteria, and 1 to 3 parts of a Chinese herbal medicinemixture. The fresh water fish forage is capable of satisfying fresh water fish nutrient requirements, promoting adsorption, preventing diseases, and improving fresh water fish immunity and productionperformance.

The invention discloses a broiler anti-vaccine immune stress agent and application thereof. The stress agent is prepared from, by mass, 2.5% to 5% of rhizoma curcumae longae, 2% to 4% of astaxanthin,2.5% to 5% of Isomaltooligosaccharide, 2.5% to 5% of threonine, 2% to 3% of methionine, 1% to 1.5% of compound multi vitamin and 76.5% to 87.5% of a carrier. The aim of the stress agent can also be achieved by preparing functional complete feed or concentrates and premix of broiler anti-vaccine immune stress. The body immune function can be enhanced by relieving neuroendocrine disorder, improvingthe multiplication capacity of splenic lymphocyte, reducing lymphocyte of the splenic lymphocyte and remarkably improving the antibody level of broilers subjected to vaccine immunity, the appetite ispromoted, and the growth performance is improved. The method is convenient for preparation use, and suitable for large-scale production.

The invention provides a preparation method of a gosling plague oral refined yolk antibody. The oral yolk antibody comprises the following effective components in parts by volume: 70-90 parts of a yolk acidification liquid containing a gosling plague yolk antibody, 0.05-0.15 part of n-octanoic acid, 0.05-0.15 part of formaldehyde, 0.1-0.2 part of sucrose and 0.01-0.02 part of skimmed milk. In a preparation process, a purification and impurity removal process of graded ultrafiltration concentration is adopted, and a hollow fiber ultrafiltration column with a molecular weight cut-off of 10kD isutilized to improve the purity of the antibody and reduce macromolecular impurities, and the incidence rate of adverse reactions in clinical application of the product is reduced. Meanwhile, the egg yolk antibody prepared by the method contains sucrose and skimmed milk, has good stability, high biological activity, slight sweet taste and good sweet taste, can reduce adverse effects on the antibodyduring oral administration, and can also improve the feed conversion rate.

Three porcine contagious Actinobacillus pleuropneumoniae comprising a serotype 1 LC strain, a serotype 5 YC strain and a serotype 7 YS strain are obtained in the invention; and a porcine contagious pleuropneumonia trivalent inactivated vaccine and its preparation method are obtained through the reasonable preparation of inactivated antigens of the above three strains to effectively prevent and control the propagation and the burst of porcine contagious pleuropneumonia in a culturing farm. The invention also provides a porcine contagious pleuropneumonia trivalent inactivated vaccine using an aqueous adjuvant. The porcine contagious pleuropneumonia trivalent inactivated vaccine furthest reduces the stress response during injection, and continuously improves the immune protection effect, so the porcine contagious pleuropneumonia trivalent inactivated vaccine is especially suitable for the large-scale application in Chinese culturing farms.