The present invention discloses a preparation method for a poultry vaccine-specific pig
spleen transfer factor. The preparation method comprises: selecting poultry vaccine
antibody-positive pigs, and slaughtering to obtain pig
spleen; carrying out
cold preservation; thawing the pig
spleen; removing
fascia;
mincing; homogenizing; filling into a
bottle, and storing; carrying out repeated freezing and thawing; carrying out
centrifugation; carrying out
microfiltration; carrying out ultra-
filtration; inactivating; adjusting the pH value; adjusting an
osmotic pressure; sterilizing; and testing quality. According to the present invention, in the homogenizing step, the pH value of water for injection is adjusted to 4-6 so as to easily increase yields of
ribose and polypeptides; the box type member
package tangential flow
filtration is adopted, such that the residual
dead volume of the
system is small, and linear enlargement production is easily achieved; the
transfer factor crude product is subjected to tangential flow nano-
filtration through a 1-3 KD box type member
package to prepare the
high concentration transfer factor so as to increase a use effect;
phenol red is adopted as a pH value indicator, such that customers easily observe the pH value of the transfer factor; and beta-
propiolactone is adopted to replace the traditional
formaldehyde to inactivate the transfer factor, and the
osmotic pressure adjustment process is added so as to easily achieve transfer factor and vaccine combined immunization.