Preparation method and application of pig small intestine compound antibacterial peptide

Abstract

The invention discloses a preparation method of a recombinant antimicrobial peptide extracted from a small intestine of a pig and an application thereof. The preparation method comprises the following steps: mashing, homogenizing and repeatedly freezing and thawing the small intestine tissue of the pig with a meat grinder and a colloid mill, and preliminarily crushing cells; then performing the water boiling heat treatment, and extracting with acetic acid solution to obtain a large amount of antimicrobial peptides; centrifuging to enable the quantity of other proteins to be reduced greatly, filtering the supernate obtained through centrifuging by a tangential flow micro-filtration membrane and a tangential flow ultrafiltration membrane sequentially, adjusting the pH value, filtering the supernate by a tangential flow nanofiltration membrane, concentrating and dialyzing to desalt, and disinfecting after adjusting the osmotic pressure to obtain the recombinant antimicrobial peptide extracted from the small intestine of the pig. According to the invention, the application of the recombinant antimicrobial peptide adopts effects of the antimicrobial peptide on aspects of mucosal immunity, immunopotentiators, novel antibiotics and the like, and the mucosal immunity character of the antimicrobial peptide can play an important role in the prevention and control of transmissible gastroenteristis, epidemic diarrhea and mycoplasma gallisepticum; the antimicrobial peptide replaces traditional antibiotics, and is remarkable in effect.

Description

technical field [0001] The invention belongs to the fields of animal husbandry and veterinary medicine, biological products, and biopharmaceuticals, and specifically relates to a preparation method and application of a pig small intestine compound antibacterial peptide. Background technique [0002] Antimicrobial peptides are short peptides produced by biological organisms during defense against pathogenic microorganisms and some malignant cells. Some international literatures use "antimicrobial peptides" (AMPs) or "peptide antibiotics". Antimicrobial peptides have antibacterial effects, and some also have antiprotozoal, antifungal, antiviral, and inhibitory or killing effects on tumor cells and parasites. It has the characteristics of small molecular weight (3-10 KD), good water solubility, heat resistance, and no immunogenicity. It can become a new antibacterial, antiviral, and anticancer drug with high efficiency, low toxicity, and no residual hazards. [0003] At prese...

AI Technical Summary

Problems solved by technology

Microorganisms in a hypertonic solution are prone to plasmolysis, shrinkage and death; on the contrary, if they are in a hypotonic solution, they are prone to absorb water, swell and die

[0011] The patent application number 201210305972.2 discloses a preparation method of porcine small intestine antibacterial peptide PR39, which uses ultrasonic cracking to release proteins in the intestinal mucosa, and uses separation column chromatography to remove most of the foreign proteins to obtain antibacterial peptides. A single PR39 antimicrobial peptide was extracted from this method, the process is complicated, the yield is not high, and it is not suitable for large-scale production, and the concentration of the obtained porcine small intestine antimicrobial peptide PR39 is not high

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