4 results about "Antiprotozoal" patented technology

This invention discloses a method for preparing the multifunctional bioactive peptide Aj-UBL from sea cucumber, relating to the fields of biotechnology and biomedicine. Using the Aj-UBL coding sequence from sea cucumber as a template, the method involves PCR amplification, recombinant expression vector construction, engineered bacterial induction expression, bacterial cell disruption, affinity chromatography purification, and identification to obtain the target peptide possessing triple activities of antibacterial, antiprotozoal, and antitumor activity. This invention employs an optimized coupling process of genetic engineering and affinity chromatography to achieve high-purity, high-quality, and stable product preparation, while being environmentally friendly and free from drug resistance risks. It not only solves the problem of coordinated disease control in sea cucumber farming but also expands the high-value application of sea cucumber resources in the biomedical field, filling the technological gap in the large-scale preparation of multifunctional bioactive peptides from sea cucumber and the biological control of ciliates, possessing significant theoretical and practical application value.

The invention belongs to the field of veterinary drugs, and particularly relates to a Tibetan veterinary drug composition and application of the Tibetan veterinary drug composition in treatment of infectious diarrhea of yaks. The composition comprises nine types of Tibetan veterinary medicinal plants including Geranium tanguticum, Swertia speckled, delphinium, dracocephalum tanguticum, safflower, Sowthistle, trogopterus dung or charred Wuling, licorice, Forsythia suspensa leaf and Scutellaria baicalensis. The Tibetan veterinary drug composition contains antibacterial, anti-inflammatory, antiviral, antiprotozoal and other active components, and shows relatively strong corrosion resistance besides the pharmacological activity, so that the addition of excessive preservatives is avoided, the relatively long storage time is ensured, and the Tibetan veterinary drug composition has dual effects. The medicine components are administered in an oral liquid form to form a safe, efficient, durable and convenient Tibetan veterinary medicine composition, and the Tibetan veterinary medicine composition can be used for treating diarrhea diseases of yaks of different age groups caused by single infection and mixed infection of bacteria, viruses and parasites.

Provided is a broad-spectrum antiprotozoal polypeptide, an amino acid sequence of which is formed by sequentially connecting a transduction protein domain sequence, a linker protein sequence, and a mutant APLV4-VP protein sequence, wherein the transduction protein domain sequence is an HIV-TAT transduction protein domain sequence, the linker protein sequence is a flexible linker protein sequence, and the mutant APLV4-VP protein sequence comprises a mutant VP4 protein and original VP2, VP3 and VP1 protein sequences. Also provided are preparation of the polypeptide and an antiprotozoal use of the polypeptide in vivo and / or in vitro in animals. On the basis of a mutant APLV4-VP protein, the feasibility of transmembrane transduction of the mutant APLV4-VP protein is realized by means of a transduction protein domain, so that an artificial antiprotozoal polypeptide is designed; the polypeptide can be used for broad-spectrum antiprotozoal, has the effect not inferior to that of antibiotic drugs, and would not cause drug resistance.

The present invention relates to the fields of gene recombination technology and pharmaceutical preparations, and provides an anticoccidial fusion protein, the amino acid sequence of which is composed of a broad-spectrum antiprotozoal polypeptide APP amino acid sequence, an oxidative stress-sensitive linker amino acid sequence, a flexible linker amino acid sequence, and a lignin peroxidase mutant LiPM amino acid sequence connected in sequence; the amino acid sequences of the broad-spectrum antiprotozoal polypeptide APP, the oxidative stress-sensitive linker, the flexible linker, and the lignin peroxidase mutant LiPM are shown in SEQ ID NOs. 1 to 4, respectively. The present invention also provides a preparation method and application of the above-mentioned anticoccidial fusion protein. The present invention is designed based on APP and LiPM. When used as an insecticide, the outer layer of the oocyst wall can be enzymatically decomposed by LiPM, while APP can enter the inner layer of the eggs to effectively kill the eggs, thereby achieving a better anticoccidial effect.