Preparation method and application of swine vaccine specific swine spleen transfer factor (TF)

A technology of transfer factor and specificity, which is applied in the field of preparation of porcine vaccine-specific porcine spleen transfer factor, can solve the problems of difficult large-scale production, TF is not suitable for pig vaccine diluent and immune adjuvant, time-consuming and other problems, and achieves reduction The effect of the number of freeze-drying operations, the reduction of freeze-drying volume, and the reduction of production costs

Active Publication Date: 2014-02-12
派生特(福州)生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Infection of suckling piglets within 7 days of age is the most common. In severe cases, it can lead to the disease of the whole group, resulting in the death of a large number of suckling piglets. The weight gain of nursery pigs and fattening pigs slows down, causing great losses to the pig industry.
The Lawrence method is routinely used, that is, the transfer factor is recovered by two dialysis methods. This method is simple to operate, but the production cycle is long, time-consuming, wasteful, ineffective and labor-intensive.
In addition, even if the tangential flow filtration method is adopted, hollow fiber membranes are mostly used for tangential flow filtration. The hollow fiber membranes are difficult to clean, and the entire component needs to be repl

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  • Preparation method and application of swine vaccine specific swine spleen transfer factor (TF)
  • Preparation method and application of swine vaccine specific swine spleen transfer factor (TF)
  • Preparation method and application of swine vaccine specific swine spleen transfer factor (TF)

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preparation example Construction

[0075] The preparation method of pig vaccine-specific pig spleen transfer factor, it comprises the following steps:

[0076] 1) Feed healthy pigs with white lentils at a dosage of 30-60g per pig per day for 15-30 days, and then immunize healthy pigs fed with white lentils with more than one pig vaccine, each pig vaccine immunized twice Above, the time interval between two adjacent immunizations is more than 7 days, and 14-28 days after the last pig vaccine immunization, detect pig vaccine antibodies, select pigs with positive pig vaccine antibodies for slaughter, and harvest pig spleens;

[0077] 2) Low-temperature preservation: the harvested pig spleen is immediately stored below -40°C, and the storage time does not exceed 20 days;

[0078] 3) Thawing pig spleen: thaw the low-temperature preserved pig spleen through running water. The thawing temperature is lower than 37°C. After thawing, wash it with purified water for 3-5 times, and discard the hardened and necrotic pig spl...

Embodiment 1

[0149] The preparation method of pig vaccine-specific pig spleen transfer factor, it comprises the following steps:

[0150] 1) Feed healthy pigs with white lentils at a dosage of 30g per pig per day for 18 days, then immunize the healthy pigs fed with white lentils with porcine reproductive and respiratory syndrome vaccine more than 3 times, and immunize pigs twice adjacent The time interval of PRRS vaccine is 7 days. 28 days after the last porcine reproductive and respiratory syndrome vaccine immunization, porcine reproductive and respiratory syndrome antibody is detected, and pigs with positive porcine reproductive and respiratory syndrome antibody are selected for slaughter and harvested. pig spleen;

[0151] 2) Low-temperature preservation: the harvested pig spleen is immediately stored at -40°C, and the storage time does not exceed 20 days;

[0152] 3) Thawing pig spleen: thaw the cryopreserved pig spleen through running water, the thawing temperature is lower than 37°C...

Embodiment 2

[0177] The preparation method of pig vaccine-specific pig spleen transfer factor, it comprises the following steps:

[0178] 1) Feed healthy pigs with white lentils at a dosage of 50g per pig per day for 21 days, and then immunize the healthy pigs fed with white lentils with swine fever vaccine and porcine pseudorabies vaccine in sequence, and each pig vaccine is immunized more than 3 times , the time interval between two adjacent immunizations is 10 days, and 20 days after the last pig vaccine immunization, detect pig vaccine antibodies, select pigs positive for swine fever or porcine pseudorabies antibodies to slaughter, and harvest pig spleens;

[0179] 2) Low-temperature preservation: the harvested pig spleen is immediately stored at -80°C, and the storage time does not exceed 20 days;

[0180] 3) Thawing pig spleen: thaw the cryopreserved pig spleen through running water, the thawing temperature is lower than 37°C, wash 5 times with purified water after thawing, and disca...

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Abstract

The invention discloses a preparation method of a swine vaccine specific swine spleen transfer factor (TF). The preparation method comprises the following steps: slaughtering a swine with a positive swine vaccine antibody to harvest the swine spleen; preserving the swine spleen at low temperature; unfreezing the swine spleen; removing fasciae; mincing the swine spleen; homogenizing the pulp; filling the homogenized pulp into a bottle and storing the pulp; repeatedly freezing and thawing the pulp; centrifuging the pulp; carrying out microfiltration; carrying out ultrafiltration; carrying out inactivation; regulating the pH value; regulating the osmotic pressure; removing bacteria; and detecting the quality. The preparation method has the advantages that the pH value of water for injection is regulated to 4-6 in the pulp homogenizing step, thus being beneficial to increasing of the yields of ribose and polypeptide; a box type membrane coating is adopted for tangential flow filtration, so that the dead volumes of system residues are small and linear amplification production is easy to achieve; a 1-3KD box type membrane coating is adopted to carry out tangential flow nanofiltration on a TF crude product, thus preparing the high-concentration TF and improving the using effects; phenol red is used as an acid-base indicator, thus being convenient for clients to observe the pH value of the TF; the TF uses beta-propiolactone for inactivation instead of traditional formaldehyde and an osmotic pressure regulation process is added, thus being beneficial to combined immunization of the TF and vaccines.

Description

technical field [0001] The invention belongs to transfer factor, and in particular relates to a preparation method and application of pig vaccine-specific pig spleen transfer factor. Background technique [0002] Transfer Factor (TF) is a substance released by T lymphocytes that can transfer sensitization information. It can specifically transfer the donor's cellular immune information to the recipient, thereby enhancing the immune function of the recipient. Since the discovery of TF in the 1950s, scholars at home and abroad have conducted a lot of research on it, and introduced it into veterinary immunity and clinical treatment in 1979. At present, TF has gradually become a widely used immune enhancer. [0003] TF has no species specificity, so TF has a wide range of sources. Human TF, pig TF, cattle TF, sheep TF, goose TF, monkey TF, etc. are common in human clinical practice, while bovine TF and pig TF are common in livestock and poultry breeding. Chicken TF. [0004] T...

Claims

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Application Information

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IPC IPC(8): A61K39/42A61K39/39A61P31/14A61P31/20
Inventor 徐磊刘友霖
Owner 派生特(福州)生物科技有限公司
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