Preparation method of gosling plague oral refined yolk antibody

Abstract

The invention provides a preparation method of a gosling plague oral refined yolk antibody. The oral yolk antibody comprises the following effective components in parts by volume: 70-90 parts of a yolk acidification liquid containing a gosling plague yolk antibody, 0.05-0.15 part of n-octanoic acid, 0.05-0.15 part of formaldehyde, 0.1-0.2 part of sucrose and 0.01-0.02 part of skimmed milk. In a preparation process, a purification and impurity removal process of graded ultrafiltration concentration is adopted, and a hollow fiber ultrafiltration column with a molecular weight cut-off of 10kD isutilized to improve the purity of the antibody and reduce macromolecular impurities, and the incidence rate of adverse reactions in clinical application of the product is reduced. Meanwhile, the egg yolk antibody prepared by the method contains sucrose and skimmed milk, has good stability, high biological activity, slight sweet taste and good sweet taste, can reduce adverse effects on the antibodyduring oral administration, and can also improve the feed conversion rate.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a preparation method of oral refined egg yolk antibody against gosling plague. Background technique [0002] With the rapid development of the human catering industry and the continuous improvement of consumption levels, food derived from waterfowl animals is favored. The amount of waterfowl breeding is expanding day by day, but the waterfowl breeding methods are relatively scattered and the breeding mode is relatively open, resulting in an increasing probability of many poultry diseases occurring in waterfowl. Gosling plague is an acute septic infectious disease of goslings caused by gosling parvovirus. The sick goose has obvious neurological symptoms and high mortality, which has a great impact on the development of the goose industry. Gosling plague can infect not only geese, but also other poultry such as muscovy ducks to a certain extent. ...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a preparation method of gosling plague oral refined egg yolk antibody, which solves the inconvenient problems of conventional egg yolk antibody injection, such as large immune stress, adopts the purification and impurity removal process of graded ultrafiltration concentration, and utilizes The hollow fiber ultrafiltration column with a molecular weight cut-off of 10kD improves the purity of the antibody, reduces macromolecular impurities, and reduces the incidence of adverse reactions in clinical application of the product. The quality of the antibody is stable, easy to use, and non-biologically toxic, which is conducive to environmental hygiene and food safety.

[0006] Another object of the present invention is to solve the problem of poor activity and stability of the yolk antibody in the prior art. After adding sucrose and skim milk in the present invention, a protein film protection is formed, which has good stability, high biological activity, micro Sweet and palatable, it can reduce the adverse effect on antibodies when taken orally, and can also improve the feed conversion rate

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