40results about How to "Simplified immunization program" patented technology

The invention provides a vaccine composition for preventing and treating porcine circovirus type 2, haemophilus parasuis and mycoplasma hyopneumoniae infection. The vaccine composition comprises an inactivated porcine circovirus type 2 antigen, inactivated haemophilus parasuis, inactivated mycoplasma hyopneumoniae and a vaccine adjuvant. The vaccine composition disclosed by the invention can realize the aim of preventing three diseases including a porcine circovirus disease, mycoplasma pneumonia, a haemophilus parasuis disease by one injection of the vaccine; the content of antigen is 1/2 of the content of a common single-vaccine antigen when the vaccine composition disclosed by the invention is prepared by mixing the three antigens; and compared with the existing condition that three injections of single vaccine are injected to prevent three infectious diseases, the technical scheme disclosed by the invention is economical and practical, reduces the production cost, simplifies an immune procedure and reduces the epidemic prevention cost.

The invention provides a multivalent pneumococcal capsular polysaccharide composition as well as a preparation method and application thereof. The multivalent pneumococcal capsular polysaccharide composition contains a serotype 6A and at least one extra serotype selected from the group consisting of 1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F and 33F. The multivalent pneumococcal capsular polysaccharide composition provided by the invention can be used for inducing an organism to generate humoral immunity, can generate a relatively good protecting effect for infectious diseases caused by the 24 common serotype pneumococcuses and is wide in immunity coverage rate and better in effect as comparison with various existing pneumococcal polysaccharide vaccines and conjugate vaccines sold on the market.

The invention provides a Zika-virus-and-yellow-fever-virus combined inactivated vaccine, and belongs to the technical field of biological product preparing. 0.5 microgram/ml-10 microgram/ml of yellowfever viruses and 0.5 microgram/ml-10 microgram/ml of Zika viruses are contained in the combined inactivated vaccine. The invention also provides a preparing method for the Zika-virus-and-yellow-fever-virus combined inactivated vaccine; the preparing method includes the steps of Zika-virus and yellow-fever-virus inoculating, purifying and inactivating, wherein yellow-fever-virus inoculated MOI is0.01 PFU/ml to 1 PFU/ml, and Zika-virus inoculated MOI is 0.001 CCID<50>/ml to 0.1 CCID<50>/ml. By means of the Zika-virus-and-yellow-fever-virus combined inactivated vaccine, the Zika viruses and theyellow fever viruses can be immunized at the same time, infection and anaphylaxis which are caused by an attenuated vaccine are avoided, and the Zika-virus-and-yellow-fever-virus combined inactivatedvaccine has the good capacity for controlling diseases caused by the Zika viruses and the yellow fever viruses.

The present invention provides a trivalent inactivated vaccine of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, and porcine pseudorabies virus. Before inactivation, the contents of the three viruses are respectively greater than 10<8.5>TCID[50]/ml 10<6.0>TCID[50] /ml and 10<8.0>TCID[50]/ml; And after the inactivation, the volume ratio of the three antigens is 1:1:1. According to the present invention, via a large number of detailed tests, the contents and ratio of the three viral antigens are selected; and immune effects are measured in a large number of experimental animals and swine themselves, to ensure that the phenomenon of immune interference does not occur among the various immune components in the multivalent vaccine. Compared with the existing three individual vaccines of the same three virus, wherein three injections are need to prevent the three diseases caused by the three virus by using the three individual vaccines, the trivalent inactivated vaccine of the present invention is economical and practical, and simplifies the immunization procedure, and reduces the cost of epidemic prevention. The present invention realizes preparation and application of multivalent inactivated vaccine of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, and porcine pseudorabies virus, which has not been achieved for all the time in the field.

The present invention relates to recombinant chicken Marek's disease virus transfer vector and its application. The transfer vector pUS2-LacZ features that the recombinant plasmid pUS2 has US2 gene segment substituted with CMZ immediate early promoter, LacZ gene, transcription termination signal SV40polyA, ampicillin and neomycin marked gene and one polyclonal site gene. The transfer vector may be homogeneously recombined with MDV vaccine virus to obtain recombinant virus MDV of LacZ marked gene, and has polyclonal site capable of inserting foreign virus gene to obtain recombinant plasmid. Through further homogeneous recombination, purification and screening, recombinant virus is obtained, and the recombinant virus may be prepared into vaccine with effect of resisting several kinds of virus.

The invention relates to a porcine circovirus type 2, porcine reproductive and respiratory syndrome bivalent vaccine and a preparation method thereof. The bivalent vaccine is a lyophilized vaccine, and comprises a porcine circovirus type 2 SH strain inactivated virus and a respiratory syndrome virus R98 strain live virus, while an adjuvant is added into the vaccine diluent, so that the immunizing effect of the vaccine can be further improved. Immune interference does not exist among the bivalent lyophilized vaccine antigens, and the quality inspection of the vaccine finished product can achieve the quality standard of a single vaccine, and the immune protection effect of the vaccine finished product is better than that of a single vaccine; the bivalent vaccine can be used for simplifying the immune procedure, reducing immune cost and reducing the stress reaction of an immunized pig, and thus the bivalent vaccine has great market application values.

The invention relates to an anti-porcine pseudorabies (PRV) and swine flu (SIV) vaccine composition. Besides porcine pseudorabies and swine flu, the composition can additionally prevent clinical manifestations of porcine respiratory disease complex (PRDC) caused by infection of other pathogenic causes. Furthermore, the invention provides application of the vaccine composition for preparing a medicine in preventing and/or controlling immunized pig PRDC disease. The composition not only avoids interference between antigens in a process of using but also achieves a surprising preventive effect on the porcine respiratory disease complex. Therefore, the vaccine composition disclosed by the invention can simplify an immune procedure and achieve an effect of preventing two diseases by one dosage. The anti-porcine pseudorabies and swine flu vaccine composition, after immunizing pigs, can effectively prevent spread of the PRDC.

The invention provides a Mycoplasma hyorhinis strain LYH, and a vaccine composition prepared from the Mycoplasma hyorhinis strain LYH, in particular to a vaccine composition comprising the Mycoplasma hyorhinis and Mycoplasma hyopneumoniae. The vaccine composition can be effective in prevention and treatment of swine enzootic pneumonia caused by Mycoplasma hyorhinis, Mycoplasma hyopneumoniae single infection or mixed infection. Especially in the circumstance of mixed infection, immune effect of the vaccine composition significantly exceeds that of each single vaccine.

The invention relates to a vaccine composition for resisting pig mycoplasma pneumonia and infectious pleuropneumonia and a preparation method. The vaccine composition comprises pig mycoplasma hyopneumoniae antigens and pig actinobacillus pleuropneumoniae antigens, is simple in immunization procedure, not only is capable of generating antibodies, but also has the functions of toxicity attack protection and effectively controlling pig mycoplasma pneumonia and infectious pleuropneumonia. The vaccine composition has the immunization effect same to an independently injected vaccine, is small in side reaction, long in immunization period, less in consumed time, less in consumed labor, small in encroaching on a pig. The vaccine composition is simple in production technology, low in immunization cost and strong in practicality.

The invention relates to a pig pseudorabies live vaccine-pig mycoplasma pneumonia live vaccine composition and the application thereof. When the vaccine composition is applied to immune animals, two vaccines can be obtained to achieve different immune effects, continuous stress on inoculated animals caused by independent immunity can be avoided, immune procedures are simplified, and immunization workload is reduced for a pig farm.

The invention provides tilmicosin injection as well as a preparation method and an application thereof. The tilmicosin injection is prepared from the following components in parts by weight: 10-50 parts of tilmicosin, 1-5 parts of astragaloside, 0.5-2 parts of ATP, 5-20 parts of a cosolvent, 0.1-0.5 part of an antioxidant, and 15-30 parts of a stabilizer. The tilmicosin injection is stable in property, remarkable in effect, and small in irritation and safe to the injected part, and can be mixed with inactivated vaccines for injection, so that the clinic application of the tilmicosin injection is expanded.

The invention discloses a porcine circovirus, porcine pseudorabies virus and mycoplasma triple inactivated vaccine which comprises an antigen and a vaccine adjuvant, the antigen is composed of a porcine circovirus type 2 antigen, a porcine pseudorabies virus antigen and a mycoplasma antigen, the porcine circovirus type 2 antigen is a purified, concentrated and inactivated porcine circovirus type 2 protein antigen solution, and the content of Cap protein is more than or equal to 160 [mu]g/ml; the porcine pseudorabies virus antigen is a purified, concentrated and inactivated porcine pseudorabies virus protein antigen solution, and the content of the Cap protein is more than or equal to 160 [mu]g/ml; the mycoplasma antigen is an inactivated mycoplasma protein antigen solution, and the content of the Cap protein is more than or equal to 160 [mu]g/ml; and the vaccine adjuvant is composed of a water-based high-molecular polymer adjuvant and a composite polysaccharide immunopotentiator. Foreign protein is removed through clarification filtration and ultrafiltration concentration, and the side reaction probability of the vaccine is greatly reduced; and three-proofing can be achieved through one needle, so that the number of immunization times and stress are reduced. The method is economical and practical, the immunization procedure is simplified, and the epidemic prevention cost is reduced.

The invention discloses an immune combined preparation and a preparation method and an application thereof, wherein the immune combined preparation comprises a streptococcus pneumoniae capsular polysaccharide-CRM197 protein conjugate and a haemophilus influenzae B capsular polysaccharide-CRM197 protein conjugate, wherein the mass ratio of a haemophilus influenzae B capsular polysaccharide to a CRM1197 protein is (0.3-1):1. The mass content of the haemophilus influenzae type B capsular polysaccharide is 10-15 [mu]g/dose. The combined vaccine can effectively induce a body to produce high-titer and specific antibodies against the selected serotype streptococcus pneumoniae polysaccharide and haemophilus influenzae type B capsular polysaccharide; and no interference exists between antigen components, and higher immune effect is achieved by screening the combination way; after the vaccine is prepared, the number of vaccinations can be reduced, the immune procedure is simplified, and human and animal diseases caused by the bacteria are effectively prevented.

The invention provides a vaccine composition. The vaccine composition comprises an immunization amount of duck tembusu virus antigen, an immunization amount of duck plague virus antigen and one or several adjuvants acceptable in veterinary medicine. The vaccine composition not only can simplify the immunization procedure achieving the purpose of preventing two diseases by injection once, but also can generate protective immunity against duck tembusu virus and duck plague and the phenomena of laying rate decrease or even zero laying rate and mass mortality for a duck group which result from mixed infection of the two diseases can also be avoided.