40results about How to "Simplified immunization program" patented technology

The invention provides a multivalence pneumococcus capsular polysaccharide-protein conjugate composition and a preparation method thereof. The conjugate composition is prepared from capsular polysaccharides of pneumococcus of 24 different serotypes and a carrier protein in a covalence connection manner, wherein the 24 different serotypes are 1, 2, 3, 4, 5, 6A, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F and 33F. The conjugate composition is good in adsorption effect and stability, has multiple immunogenicity and protection properties aiming at invasion of pneumococcus of the 24 serotypes, and is superior to the low-valence pneumonia composition in the market, and the immune response is higher than that of uncombined compositions. By inoculating a multivalence pneumococcus capsular polysaccharide conjugate vaccine prepared from the conjugate composition, the inoculation injection times can be reduced, the immunization procedure can be simplified, and meanwhile human beings and animals can be effectively prevented from diseases resulted from the pneumococcus of the 24 serotypes, and the conjugate composition is wide in coverage range and good in immune effect.

The invention discloses a preparation method for a newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine and belongs to the technical field of biological products for veterinary use. The preparation method comprises preparation of a newcastle disease antigen liquid, preparation of an infectious bursal disease antigen liquid and preparation of the newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine. The vaccine prepared by the invention has a dual characteristic of water in oil and oil in water and has the advantages of integration of a slow release function of the conventional vaccine and the jointed functions of a water-soluble immunopotentiator, Chinese herbal medicinal polysaccharide, an analgesic and the like. According to the preparation method, the immunity of an organism is greatly improved, and the humoral immunity is improved; the newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine can stimulate the organism to generate efficient neutralizing antibodies, and the disease prevention ability of the organism is improved. According to the newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine, adverse reactions of chickens caused by fractional immunization of multiple vaccines are avoided, the immunization procedure is simplified, and the investment cost is reduced. The vaccine preparation by the invention is suitable for chickens of all ages; one-day chicks are immunized, so that immunity failures of a live vaccine caused by maternal interference resistance or immunosuppression caused by high toxicity can be avoided; the newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine is safely and effectively used.

The invention provides a Zika-virus-and-yellow-fever-virus combined inactivated vaccine, and belongs to the technical field of biological product preparing. 0.5 microgram / ml-10 microgram / ml of yellowfever viruses and 0.5 microgram / ml-10 microgram / ml of Zika viruses are contained in the combined inactivated vaccine. The invention also provides a preparing method for the Zika-virus-and-yellow-fever-virus combined inactivated vaccine; the preparing method includes the steps of Zika-virus and yellow-fever-virus inoculating, purifying and inactivating, wherein yellow-fever-virus inoculated MOI is0.01 PFU / ml to 1 PFU / ml, and Zika-virus inoculated MOI is 0.001 CCID<50> / ml to 0.1 CCID<50> / ml. By means of the Zika-virus-and-yellow-fever-virus combined inactivated vaccine, the Zika viruses and theyellow fever viruses can be immunized at the same time, infection and anaphylaxis which are caused by an attenuated vaccine are avoided, and the Zika-virus-and-yellow-fever-virus combined inactivatedvaccine has the good capacity for controlling diseases caused by the Zika viruses and the yellow fever viruses.

A new flow method adeno-quadruplet virus-like particle and its preparation method and application belong to the field of quadruple vaccine research and development. Rescue, preparation and purification of chimeric virus-like particles, and finally obtain chimeric virus-like particles NDV‑AIV‑IBDV‑FAdV4 cVLPs. The new flow method AAV-like particle prepared by the preparation method of the present invention has the following advantages: the new flow method AAV-like particle can repeatedly display foreign antigens with high density, and has strong immunogenicity; It does not contain viral nucleic acid, green and safe, which is conducive to the purification of new flow glands, and conforms to the modern green and safe breeding concept; the vaccine seed virus is matched with the popular virus strain; one shot for multiple prevention, simplifying the vaccine immunization procedure, avoiding a variety of Mutual interference of vaccines; suitable for large-scale suspension culture and mass production.

The present invention provides a double inactivated vaccine of bovine infectious rhinotracheitis virus and mycoplasma bovis, a preparation method thereof, and suspended MDBK cells used therein. The invention provides suspension MDBK cells, and on this basis, establishes a method for suspending IBRV antigen virus liquid. The IBRV antigen virus liquid obtained by the method has the characteristics of high antigen content, large antigen batch, stable batch, etc. The side reactions caused by the bovine serum added to the culture medium can save the economical cost while producing a safer and more efficient vaccine. At the same time, the IBRV and MB double inactivated vaccine of the present invention can prevent two diseases with one injection, simplify the immunization procedure, reduce the number of immunizations of animals, reduce the stress of animals, save labor, and further reduce the cost of animal breeding.

The invention provides a vaccine composition for preventing and treating porcine circovirus type 2, haemophilus parasuis and mycoplasma hyopneumoniae infection. The vaccine composition comprises an inactivated porcine circovirus type 2 antigen, inactivated haemophilus parasuis, inactivated mycoplasma hyopneumoniae and a vaccine adjuvant. The vaccine composition disclosed by the invention can realize the aim of preventing three diseases including a porcine circovirus disease, mycoplasma pneumonia, a haemophilus parasuis disease by one injection of the vaccine; the content of antigen is 1 / 2 of the content of a common single-vaccine antigen when the vaccine composition disclosed by the invention is prepared by mixing the three antigens; and compared with the existing condition that three injections of single vaccine are injected to prevent three infectious diseases, the technical scheme disclosed by the invention is economical and practical, reduces the production cost, simplifies an immune procedure and reduces the epidemic prevention cost.

A provided vaccine composition for preventing and treating porcine respiratory syndrome comprises immunization amount of porcine influenza virus antigen, immunization amount of porcine pneumonia mycoplasma antigen, immunization amount of haemophilus parasuis antigen, and an adjuvant. The vaccine composition is capable of effectively preventing and controlling porcine respiratory syndrome caused by mixed infection of the three kinds of pathogeny, and also is capable of reducing vaccine inoculating frequency and avoiding unavailable full-access immunization caused by missed inoculation.

The invention provides a triple inactivated vaccine for porcine epidemic diarrhea (PED), porcine transmissible gastroenteritis (TGE) and porcine delta coronavirus (PDCoV) and a preparation method of the triple inactivated vaccine, before inactivation, the contents of the three viruses are greater than or equal to 10<7.0>TCID50 / mL, and after inactivation, the volume ratio of the antigens is 1 to 1to 1. With the triple inactivated vaccine disclosed by the invention, the problem that an effective multivalent vaccine for preventing and treating three diseases including porcine epidemic diarrhea (PED), porcine transmissible gastroenteritis (TGE) and porcine delta coronavirus (PDCoV) is not available on the market is solved, especially, the problem that the vaccine for the porcine delta coronavirus (PDCoV) epidemic in recent years is not available is solved. The triple inactivated vaccine provided by the invention is economical and practical, the immunizing procedure is simplified, the epidemic prevention cost can be effectively reduced, and a novel method for simultaneously preventing the occurrence of the three diseases is provided for domestic breeding enterprises.

The invention relates to a pig pseudorabies live vaccine-pig mycoplasma pneumonia live vaccine composition and the application thereof. When the vaccine composition is applied to immune animals, two vaccines can be obtained to achieve different immune effects, continuous stress on inoculated animals caused by independent immunity can be avoided, immune procedures are simplified, and immunization workload is reduced for a pig farm.