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A vaccine composition for preventing and treating porcine respiratory syndrome, its preparation method and application

A vaccine composition and respiratory technology, applied in the field of veterinary vaccines, can solve the problems that antibacterial drugs are difficult to work, and achieve the effects of reducing production costs and epidemic prevention costs, low cost, and cost reduction

Active Publication Date: 2016-09-07
PULIKE BIOLOGICAL ENG INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It shows that the sick pigs with these symptoms are caused by the synergistic action of these three pathogens, and once secondary infection with other pathogenic bacteria will cause more complicated diseases, various antibacterial drugs are difficult to be effective

Method used

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  • A vaccine composition for preventing and treating porcine respiratory syndrome, its preparation method and application
  • A vaccine composition for preventing and treating porcine respiratory syndrome, its preparation method and application
  • A vaccine composition for preventing and treating porcine respiratory syndrome, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Preparation of three antigens in the vaccine composition of embodiment 1, swine influenza virus, mycoplasma hyopneumoniae, Haemophilus parasuis

[0044] 1. Preparation of swine influenza virus (ZJS strain and WX strain) antigen

[0045] Spinner bottle cell culture method. Inoculate SIV H1N1 subtype ZJS strain and H3N2 subtype WX strain virus seeds into monolayer MDCK cells (purchased from ATCC, No. CRL-34) according to the inoculum amount of M.O.I=0.1, absorb at 37°C for 30 minutes, add DMEM cell maintenance solution containing 3% (v / v) calf serum, cultured at 37°C with rotation (10-12 revolutions / hour). Observe 1-2 times a day, the cells grow well, culture at 37°C for 2-5 days, harvest the cells and cell liquid, freeze and thaw 3 times, separate the two virus liquids (ZJS strain and WX strain) with hollow fibers (pore size 1.2 μm) Filter with a 0.45μm) filter column to remove cell debris, then add 0.2% to 0.3% formaldehyde solution to inactivate at 37°C for 18h, and ...

Embodiment 2

[0071] Embodiment 2, preparation of swine influenza virus antigen, Haemophilus parasuis antigen, Mycoplasma swine pneumonia antigen vaccine composition:

[0072] In order to compare the immune effects of vaccine compositions with different antigen content, two kinds of adjuvants were selected to prepare the vaccine composition, namely Montanide TM Gel (produced by SEPPIC, France), aluminum glue, white oil, squalene, and each adjuvant were used to prepare vaccine compositions for swine influenza virus antigen, Haemophilus parasuis antigen, and mycoplasma swine pneumonia antigen respectively. The specific configuration is as follows:

[0073] 1. Use Gel adjuvant vaccine

[0074] Preparation method: the formula is as shown in Table 2. Take the swine influenza antigen, Haemophilus parasuis antigen and Mycoplasma hyopneumoniae antigen prepared in Example 1. The concentrated five kinds of antigen solutions were prepared into mixed antigen solutions or directly prepared into antig...

Embodiment 3

[0100] Embodiment 3, the immune effect evaluation of vaccine composition to pig

[0101] 1 Test piglets immunized with vaccine

[0102] Vaccine composition (vaccine 1, vaccine 2, vaccine 3, vaccine 4, vaccine 5, vaccine composition (vaccine 1, vaccine 2, vaccine 3, vaccine 4, vaccine 5, Vaccine 6, Vaccine 7, and Vaccine 8) were used for testing after passing the sterility inspection.

[0103] A total of 45 groups of 225 piglets aged 14-21 days were selected, 5 pigs / group. There were 240 immune groups. The triple vaccine composition of the present invention with each adjuvant was used to immunize 10 groups of 50 piglets. There were 5 groups of 25 piglets in the control group. The specific groups of immunized piglets and the number of immunized piglets are shown in Table 6; according to the groups, each pig was injected with 2ml of the corresponding inactivated vaccine in the neck muscle, and 2ml was inoculated again 14 days after immunization; at the same time, 5 groups of ...

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Abstract

A provided vaccine composition for preventing and treating porcine respiratory syndrome comprises immunization amount of porcine influenza virus antigen, immunization amount of porcine pneumonia mycoplasma antigen, immunization amount of haemophilus parasuis antigen, and an adjuvant. The vaccine composition is capable of effectively preventing and controlling porcine respiratory syndrome caused by mixed infection of the three kinds of pathogeny, and also is capable of reducing vaccine inoculating frequency and avoiding unavailable full-access immunization caused by missed inoculation.

Description

technical field [0001] The invention relates to a vaccine composition for preventing and treating porcine respiratory syndrome, belonging to the field of veterinary vaccines. Background technique [0002] Swine influenza (SI) is an acute and highly contagious respiratory disease caused by swine influenza virus (SIV). Clinically, it is characterized by sudden onset, high fever, cough, dyspnea, exhaustion, high morbidity, and low mortality. Because SIV can damage pig respiratory tract epithelial cells, sick pigs are prone to secondary or concurrent other viral diseases and bacterial diseases. When there are other pathogens secondary or mixed infection, the pathological changes will be serious and complicated, and most of them will die due to concurrent pneumonia, bronchopneumonia, pleurisy, porcine pneumonia, streptococcus, etc., which will bring great harm to the pig industry. Currently, SI has become a disease that harms swine populations in many countries including China ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61P31/04A61P31/16
Inventor 张许科孙进忠白朝勇
Owner PULIKE BIOLOGICAL ENG INC
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