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Preparation method for newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine

A technology for bursal disease and chicken Newcastle disease, applied to medical preparations containing active ingredients, antiviral agents, pharmaceutical formulas, etc., can solve the problems of autoimmune system disorder, not widely promoted, poor immune effect, etc., and achieve improvement Humoral immunity, improve the ability to prevent diseases, use safe and effective effect

Active Publication Date: 2015-07-15
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the clinical use of live vaccines at home and abroad, although live vaccines can play a part of the protective effect, there is a shrinkage of the bursa of Fabricius itself, which leads to the disorder of the autoimmune system. In addition, genetic engineering vaccines, antigen-antibody complex vaccines There is also a low protection rate
Inactivated vaccines can produce higher antibodies after immunizing chickens, which can effectively protect the body. However, at present, domestic inactivated bursa vaccines have not been widely promoted due to their poor immune effect or high production costs. Most of the vaccines used are expensive imported vaccines

Method used

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  • Preparation method for newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine
  • Preparation method for newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine
  • Preparation method for newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Using DF-1 cell line to culture chicken infectious bursal disease virus to prepare chicken infectious bursal disease antigen solution

[0047] (1) Passage and culture of cells for seedling production: take the chicken embryo fibroblast cell line DF-1 cell line, digest and pass the trypsin cell dispersion solution, and continue to cultivate with the cell growth solution to form a good monolayer. Passage or virus inoculation;

[0048] (2) Propagation of cytotoxic seed: take the infectious bursal bursal virus HQ strain seed for production, inoculate 1% of the volume of the maintenance solution into the chicken embryo fibroblasts that have grown into a good monolayer, and place 37 Continue to culture in the maintenance solution at ~38℃, harvest the cell fluid when the cytopathic rate reaches 75% or more;

[0049] (3) Multiplication of the venom for seedling production: Take the above-mentioned cell line culture flask that has formed a good monolayer in step (1), discar...

Embodiment 2

[0051] Example 2 Preparation of Chicken Newcastle Disease Antigen Solution with Chicken Embryo Subculture Cell Line and Bioreactor Culture

[0052] (1) Selection of bioreactor: rapid flow reactor AP-20 with a volume of 7L.

[0053] (2) Cell selection: chicken embryo passage cell line DF-1, a passage cell line suitable for the growth of chicken Newcastle disease virus, non-carcinogenic, purchased from the American Culture Collection;

[0054] (3) Selection of virus strain: The La sota strain of chicken Newcastle disease virus is selected, which can be purchased from the China Veterinary Drug Administration.

[0055] (4) Cultivation of species of cells: Use square flasks to culture DF-1 cells, generally pass 1:3 to 1:5, and digest and count the cells after they have grown into a monolayer for large-scale cell culture.

[0056] (5) Propagation of cell-adapted virus seed: Take cell-adapted Newcastle disease virus seed, inoculate it in step (4), which has grown into a monolayer of chicken em...

Embodiment 3

[0061] Example 3 Preparation of Chinese medicine polysaccharides and Chinese medicine flavonoids

[0062] a. According to 20 parts of Achyranthes bidentata, 15 parts of Poria cocos, 25 parts of Andrographis paniculata, 30 parts of Astragalus, 20 parts of Radix Isatidis, 20 parts of Habitat, 20 parts of Angelica, 15 parts of Licorice, 20 parts of Rhubarb Weigh each Chinese medicine raw material, chop and wash After soaking in cold water overnight, add purified water 15 times the weight of the raw materials, and water bath to 90℃, and keep it at 90℃, boil for 2 hours, and stir once every 10 minutes during the boil;

[0063] b. Discard a traditional Chinese medicine residue, collect the medicinal solution, centrifuge at 10,000 rpm for 10 minutes after cooling at room temperature, discard the precipitate, and collect the supernatant;

[0064] c. The supernatant in b is subjected to alcohol precipitation, the precipitation is the crude Chinese medicine polysaccharide, and the supernatant ...

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Abstract

The invention discloses a preparation method for a newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine and belongs to the technical field of biological products for veterinary use. The preparation method comprises preparation of a newcastle disease antigen liquid, preparation of an infectious bursal disease antigen liquid and preparation of the newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine. The vaccine prepared by the invention has a dual characteristic of water in oil and oil in water and has the advantages of integration of a slow release function of the conventional vaccine and the jointed functions of a water-soluble immunopotentiator, Chinese herbal medicinal polysaccharide, an analgesic and the like. According to the preparation method, the immunity of an organism is greatly improved, and the humoral immunity is improved; the newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine can stimulate the organism to generate efficient neutralizing antibodies, and the disease prevention ability of the organism is improved. According to the newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine, adverse reactions of chickens caused by fractional immunization of multiple vaccines are avoided, the immunization procedure is simplified, and the investment cost is reduced. The vaccine preparation by the invention is suitable for chickens of all ages; one-day chicks are immunized, so that immunity failures of a live vaccine caused by maternal interference resistance or immunosuppression caused by high toxicity can be avoided; the newcastle disease and infectious bursal disease bigeminal composite inactivated vaccine is safely and effectively used.

Description

Technical field [0001] The invention belongs to the technical field of veterinary biological products, and specifically relates to a method for preparing a combined inactivated vaccine of Newcastle disease and bursal disease of chickens. Background technique [0002] Newcastle disease (ND) is a severe viral infectious disease caused by Newcastle disease virus (NDV). It is one of the most serious poultry infectious diseases in the world today, and it is also among all chicken diseases in my country. The most harmful one is classified as a Class A infectious disease by the International Veterinary Service (OIE). At present, Newcastle disease is still common throughout the country. It restricts the development of my country's poultry industry and the export of poultry products, and it also has potential harm to food safety and human health. For the control of the disease, in developed countries, the main method is to hunt and kill the virulent infection group, combined with vaccinat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61K39/17A61P31/14A61K39/12
Inventor 张秀文李阳闫艳丽刘雪王二先盛璐丝
Owner 浙江美保龙生物技术有限公司
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